Effect of lymph node metastasis on the prognosis of patients with G2 phase stage pancreatic neuroendocrine tumors / 国际外科学杂志

Objective:To investigate the effect of lymph node metastasis on the prognosis of patients with G2 phase stage pancreatic neuroendocrine neoplasm(pNEN).Methods:A retrospective case control study was conducted to analyze the case data of 368 patients with pancreatic neuroendocrine tumors in G2 phase stage from January 1, 2010 to December 31, 2016 in SEER database, including 174 males and 194 females. According to whether lymph nodes were metastatic, they are divided into lymph node non metastatic (N0) group ( n=272) and lymph node metastatic (N1) group ( n=96). The Kaplan-Meier method and Log-rank test were used to compare the overall survival rate (OS) of patients in the N0 and N1 groups. The COX proportional risk model was used to evaluate whether N stage was an independent risk factor affecting prognosis. Count data were expressed as cases and percentage(%), and Chi-square test was used for comparison between the groups. Results:Among all patients, the OS of patients in the N0 group was better than that of patients in the N1 group. The OS of N0 patients at 1, 3, and 5 years was 96.3%, 92.7%, and 85.6%, respectively, while the OS of N1 patients at 1, 3, and 5 years was 92.6%, 82.1%, and 82.1%, respectively ( P=0.014). Multivariate analysis showed that age ( HR=2.245, 95% CI: 1.126-4.475, P=0.022) and N stage ( HR=0.457, 95% CI: 0.237-0.883, P=0.020) were independent prognostic factors for G2 phase pNEN patients. Conclusion:Lymph node metastasis is one of the independent prognostic factors in patients with G2 phase stage pNEN.

Case analysis of multiple endocrine adverse reactions associated with immune checkpoint inhibitors / 中华内分泌代谢杂志

Objective:To investigate the clinical features of multiple endocrine gland dysfunction in patients with tumors after using immune checkpoint inhibitors(ICIs).Methods:Cases with two or more abnormalities of endocrine gland function after immunotherapy were collected from the Department of Endocrinology, Zhongshan Hospital, Fudan University between January 2019 and January 2022. Clinical manifestations, laboratory tests, imaging, treatment and prognosis were analyzed.Results:A total of 12 patients were included, 6 males and 6 females, aged(61.2±10.0) years old. All patients received programmed cell death protein-1(PD-1) monoclonal antibody therapy, and the time to endocrine abnormality ranged from 9 to 94 weeks after administration. All patients developed primary hypothyroidism, 11 of them had isolated adrenocorticotropic hormone deficiency, and 1 had primary adrenal insufficiency.Conclusion:ICIs can involve multiple endocrine glands simultaneously or successively, mainly manifested as primary hypothyroidism and isolated adrenocorticotropic hormone deficiency. It is essential to assess the function of the pituitary and target glands in patients treated with ICIs to improve the safety of immunotherapy.

Tacrolimus action pathways in an ointment base for hypertrophic scar prevention in a rabbit ear model

Abstract Background: Tacrolimus is used to prevent unaesthetic scars due to its action on fibroblast activity and collagen production modulation. Objectives: To evaluate the action pathways, from the histopathological point of view and in cytokine control, of tacrolimus ointment in the prevention of hypertrophic scars. Methods: Twenty-two rabbits were submitted to the excision of two 1-cm fragments in each ear, including the perichondrium. The right ear received 0.1% and 0.03% tacrolimus in ointment base twice a day in the upper wound and in the lower wound respectively. The left ear, used as the control, was treated with petrolatum. After 30 days, collagen fibers were evaluated using special staining, and immunohistochemistry analyses for smooth muscle actin, TGF-β and VEGF were performed. Results: The wounds treated with 0.1% tacrolimus showed weak labeling and a lower percentage of labeling for smooth muscle actin, a higher proportion of mucin absence, weak staining, fine and organized fibers for Gomori's Trichrome, strong staining and organized fibers for Verhoeff when compared to controls. The wounds treated with 0.03% tacrolimus showed weak labeling for smooth muscle actin, a higher proportion of mucin absence, strong staining for Verhoeff when compared to the controls. There was absence of TGF-β and low VEGF expression. Study limitations: The analysis was performed by a single pathologist. Second-harmonic imaging microscopy was performed in 2 sample areas of the scar. Conclusions: Both drug concentrations were effective in suppressing TGF-β and smooth muscle actin, reducing mucin, improving the quality of collagen fibers, and the density of elastic fibers, but only the higher concentration influenced elastic fiber organization.

Determination of Relationship between Thyroid Gland Volume and Anthropometric Indices

Objective: To sonographically determine the thyroid gland volume in normal adults in Jos University Teaching Hospital, as well as how it relates to anthropometric factors. Background:The thyroid gland is one of the largest endocrine glandsin the body weighing about 10-25g. It regulates the rate of metabolismand controls the growth and rate of function of many other systems in the body. These it does, by producing thyroid hormones, principally thyroxine(T4) and triiodothyronine(T3). The accurate estimation of the size of the thyroid is very important for the evaluation and management of thyroid disorders. Thus, knowing the normal size in a geographic location would form a baseline for detecting abnormalities. Ultrasonography is a cheap, readily available, easy to perform and non-invasive method to image the thyroid gland, hence its use in this resource-limited setting.Methods: This is a cross-sectional study of sonographic measurement of thyroid gland volume on 400 normal (healthy) adults in Jos, Plateau State, Northern part of Nigeria. All examinations were performed using LOGIC 5, a real-time ultrasound machine using a 10MHZ linear transducer and ultrasound transmission gel to act as a coupling gel. Measurements of each lobe and isthmus were obtained in longitudinal (length), transverse (width) and depth(breath) in centimetres (cm). Blood samples were taken for thyroid function tests. The weight and heights were obtained. The data obtained were statistically analyzed using SPSS software version 17. The results were presented in forms of tables, graphs and chart.Results:The mean thyroid volume for males 6.03cm3±2.22 was higher than that of females 5.62cm3±2.14. The mean right lobe volume (RLV) was 3.09cm3±1.47 and that of males and females were 3.16cm3 ±1.34 and 3.04cm3 ±1.55 respectively. The mean left lobe volume (LLV) was 2.69cm3 ± 1.37 and that of males and females were2.89cm3±1.32 and 2.57cm3±1.39 respectively. The right lobe volume was significantly greater than the left lobe(p=0.000).The total mean isthmus volume was 0.27cm3±0.31. The mean isthmus volume in males 0.3±0.28 is significantly higher than that of females 0.24±0.23(p=0.025). The BMI increases in females with increasing age. BMI and BSA are higher in males.Conclusion:The volume obtained in this study was slightly lower than those reported by previous studies in Nigerian adults. The right lobe volume was higher than that of the left and the volume was higher in males compared to females. Anthropometric parameters were noted to affect the thyroid volume

Endocrine gland-derived vascular endothelial growth factor and placenta-mediated pregnancy complication / 中华围产医学杂志

Placenta-mediated pregnancy complication (PMPC), including preeclampsia, fetal growth restriction and recurrent pregnancy loss, is caused by inadequate trophoblast invasion and abnormal remodeling of maternal spiral arteries in early pregnancy, resulting in adverse perinatal outcomes and affecting the long-term maternal and child health. However, the molecular mechanisms of PMPC remain unclear. Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) is highly expressed in human placenta and plays an important role in the development of a normal placenta through promoting placental angiogenesis and inhibiting trophoblast migration and invasion. EG-VEGF dysregulation is closely related to the pathogenesis of PMPC. This review described recent advances in EG-VEGF for better understanding of the underlying mechanism of PMPC and providing a potential biomarker for early diagnosis of PMPC.

Endocrine gland-derived vascular endothelial growth factor and placenta-mediated pregnancy complication / 中华围产医学杂志

Placenta-mediated pregnancy complication (PMPC), including preeclampsia, fetal growth restriction and recurrent pregnancy loss, is caused by inadequate trophoblast invasion and abnormal remodeling of maternal spiral arteries in early pregnancy, resulting in adverse perinatal outcomes and affecting the long-term maternal and child health. However, the molecular mechanisms of PMPC remain unclear. Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) is highly expressed in human placenta and plays an important role in the development of a normal placenta through promoting placental angiogenesis and inhibiting trophoblast migration and invasion. EG-VEGF dysregulation is closely related to the pathogenesis of PMPC. This review described recent advances in EG-VEGF for better understanding of the underlying mechanism of PMPC and providing a potential biomarker for early diagnosis of PMPC.

Promotion effect of endocrine gland-derived vascular endothelial growth factor on proliferation and migration of colon cancer LoVo cells / 吉林大学学报(医学版)

Objective: To investigate the effect of endocrine gland-derived vascular endothelial growth factor (EG-VEGF) on the malignant behavior of colon cancer LoVo cells. Methods: The LoVo cells treated with different concentrations (50, 100, and 200 μg · L-1) of EG-VEGF were used as EG-VEGF groups, and the colon cancer LoVo cells cultured with solution without EG-VEGF were regarded as control group. The proliferation activity of LoVo cells was determined by MTT method; flow cytometry (FCM) was used to detect the percentages of LoVo cells at different cell cycles in various groups; Wound scratch assay test and Transwell cell migration assay were used to detect the migration rates of LoVo cells and the number of migration cells. Results: The MTT results showed that compared with control group, the proliferation activities of LoVo cells in 50, 100, and 200 μg · L-1 EG-VEGF groups were increased significantly (P<0. 05); with the increasing of concentration of EG-VEGF, the proliferation activity was increased apparently. The FCM results showed that compared with control group, the percentage of colon cancer LoVo cells at G0/Gi phase in 100 μg · L-1 EG-VEGF group was decreased (P<0. 05) and the percentage of colon cancer LoVo cells at S phase was increased (P<0. 05), but the percentage of colon cancer LoVo cells at G2 + M phase did not change significantly. The wound scratch assay results showed that compared with control group, the migration rate of LoVo cells in 100 μg · L-1EG-VEGF group was increased (P< 0. 05). The Transwell assay results showed that compared with control group, the number of migration cells in 100 μg · L-1 EG-VEGF group was increased significantly (P<0. 05). Conclusion: EG-VEGF can obviously promote the proliferation and migration of colon cancer LoVo cells, and EG-VEGF may be associated with the malignant development of colon cancer.

How relevant are vascular endothelial growth factor and intercellular adhesion molecule in the systemic capillary leak syndrome of psoriasis?

Abstract: Psoriasis is a chronic disease, characterized by erythematous scaly lesions, presented in eight different forms: plaques, guttate, pustular, erythrodermic, inverse, nail and scalp psoriasis, and psoriatic arthritis. Its development depends on genetic factors, external stimulus and immune response alteration.1 Proinflammatory cytokines such as TNF-alpha, IL-12 and 23 may also be involved. In the worst cases, systemic complications linked to endothelial alterations may occur. A literature review was conducted for a better understanding of what roles VEGF (vascular endothelial growth factor) and ICAM-1 (intercellular adhesion molecule) have, among other cytokines, in systemic capillary leak syndrome, involved in erythrodermic and pustular psoriasis, the most unstable forms of the disease.

CT features of neuroendocrine tumor of the pancreas: report of 28 cases / 中华胰腺病杂志

Objective To observe computed tomography features of neuroendocrine tumor of the pancreas.Methods Computed tomography scans for 28 patients with pathologically proven neuroendocrine tumor of the pancreas were retrospectively analyzed.The data of tumor locations,diameters of the tumor and internal composition,pattern of enhancement,changes of biliary and pancreatic duct,and lymphatic metastasis,remote metastasis were recorded.Results A total of 32 lesions were detected,24 lesions were single lesions,while 4 lesions were multiple lesions (2 lesions within pancreas).The shapes of these lesions were nodule-like or mass-like.Eighteen lesions were located in pancreatic tail,10 in pancreatic head,2 in pancreatic body,and 2 between pancreatic tail and body.Among the lesions located in pancreatic head,pancreatic duct dilation were detected in 5 cases,bile duct dilation in 1 case,both biliary and pancreatic duct dilation in 2 cases,and no dilation in the remaining 2 cases.The diameters of the tumor ranged from 1.0 to 20.0cm (mean5.1 cm),and the size was ＜2 cmin 1 case,2 ～5 cm in 23 cases; ＞5 cm in 8 cases.After enhancement,the lesions were enhanced to different degrees,and the peak value occurred in the pancreatic phase.Twenty-three lesions invaded adjacent vessels or organs,and lymphatic metastasis was observed in 5 cases,remote metastasis were recorded in 6 cases.Conclusions Neuroendocrine tumor of the pancreas has certain features on computed tomography.It is highly likely to make the pre-operative diagnosis when clinical data is also taken into consideration.

Suprimento arterial para as glândulas adrenais em ovinos natimortos da raça Santa Inês / Arterial supply to the adrenal glands in Santa Ines stillborn sheep

As glândulas adrenais são órgãos situados no pólo cranial dos rins, constituídos internamente por córtex e medula. O objetivo deste estudo foi de estabelecer quais os vasos arteriais que respondem pelo suprimento arterial para as glândulas adrenais em ovinos da raça Santa Inês. Para tanto, foram utilizados trinta ovinos da raça Santa Inês, natimortos, de ambos os sexos, coletados em núcleos criatórios da região de Brasília ­ DF. Os animais tiveram o sistema arterial preenchido com solução aquosa a 50% de Neoprene Látex "450", corado com pigmento específico e submetidos à fixação em solução aquosa de formaldeído a 10%. Em seguida foi promovida a dissecação das artérias responsáveis pelo suprimento arterial sanguíneo das glândulas adrenais. Diante dos achados, verificou-se que a glândula adrenal direita foi irrigada pelas artérias renal, lombar I e lombar II correspondentes, e a glândula adrenal esquerda recebeu ramos das artérias celíaca, mesentérica cranial, aorta abdominal, renal esquerda, lombar I esquerda e lombar II esquerda.

The adrenal glands are situated in the cranial pole of the kidneys, made internally by the presence of a cortex and a medulla. The aim of this study was to establish that the arterial vessels to account for the arterial supply to the adrenal gland in sheep Santa Ines. To do so, we used thirty sheep Santa Ines, stillbirths, of both sexes collected in farms from the core region of Brasilia - DF. The animals had marked its arterial system by padding with an aqueous 50% Neoprene Latex "450", stained with specific pigment and still subjected to fixation with aqueous 10% formaldehyde. Then was promoted to dissection of the arteries that supplied blood pressure of the adrenal glands. Considering the findings demonstrate that the arteries renal, lumbar spine I and II were responsible for irrigation of the right adrenal gland. The abdominal aorta and the celiac, renal, mesenteric artery, lumbar spine I and II were responsible for the arterial supply of the left adrenal gland.

The incidence and clinical characteristics of gastroenteropancreatic neuroendocrine neoplasm in Chengdu City in 2010 / 中华消化杂志

Objective To investigate the incidence of gastroenteropancreatic neuroendocrine neoplasm (GEP-NEN) in Chengdu City in 2010 and summarize clinical characteristics of GEP-NEN.Methods The incidence of GEP-NEN was estimated with the data in 2010 from the databases of West China Hospital and Chengdu Health Information Centre.Results A total of 77 cases of GEP-NEN were diagnosed in West China Hospital in 2010.Ten cases from other hospitals or non-Chengdu citizenship were excluded,so a total of 67 cases were included in this study.In 2010,the incidence of GEP-NEN was 1.86/105 in Chengdu City.Among 67 GEP-NEN cases,most lesions were located in pancreas and rectum (38 cases,56.7％),followed by stomach (10 cases),esophagus (seven cases) and duodenum (four cases).Among 57 GEP-NEN cases which had pathotogial grading,26 cases (45.6 ％) had neuroendocrine carcinomas or mixed adenoneuroendocrine carcinomas when diagnosed.Conclusions In 2010,the incidence of GEP-NEN in Chengdu City is similar to the reports from other countries.Pancreas,rectum and upper gastrointestinal tract are predilection sites of GEP-NEN.The diagnosis rate of early GEP NEN needs to be raised.

Expressions and significance of two kinds of vascular endothelial growth factors of different origin in adrenocortical lesions of primary aldosteronism / 中华内分泌代谢杂志

Objective To investigate the expression and significance of the endocrine gland-derived vascular endothelial growth factor (EG-VEGF) and vascular endothelial growth factor(VEGF) in adrenocortical lesions of primary aldosteronism. Methods The expressions of EG-VEGF, and VEGF were detected by immunohistochemistry and real-time fluorescence quantitative PCR in samples of 18 cases of adrenocortical adenoma, 6 adrenocortical hyperplasia, and 8 normal adrenal cortex. The correlation between the expressions of EG-VEGF, VEGF, and clinicopathological parameters was analyzed. Results The expression of EG-VEGF or VEGF in adrenocortical adenomas was higher than that in adrenocortical hyperplasia or normal adrenal cortex ( all P＜0. 05 ), and the expression of EG-VEGF or VEGF between adrenocortical hyperplasia samples and normal adrenal cortex samples was indistinctive. There was no statistically significant correlation between EG-VEGF or VEGF expression and sex, age, blood pressure, serum potassium, plasma renin activity, except in case of serum aldosterone( P＜0.05 ). A positive correlation between EG-VEGF and VEGF ( P＜0. 01 ) was found. Conclusions EG-VEGF and VEGF may play a significant role in the formation and development of adrenocortical tumors in primary aldosteronism.

Ultrastructural analysis of the dorsal body gland of the terrestrial snail Megalobulimus abbreviatus (Becquaert, 1948) / Análise ultraestrutural da glândula corpo dorsal do caracol terrestre Megalobulimus abbreviatus (Becquaert, 1948)

The ultrastructure of the reproductive gland, dorsal body (DB), of Megalobulimus abbreviatus was analysed. Electron microscope immunohistochemistry was used to detect FMRFamide-like peptides in the nerve endings within this gland. Nerve backfilling was used in an attempt to identify the neurons involved in this innervation. In M. abbreviatus, the DB has a uniform appearance throughout their supraesophageal and subesophageal portions. Dorsal body cells have several features in common with steroid-secreting gland cells, such as the presence of many lipid droplets, numerous mitochondria with tubular cristae and a developed smooth endoplasmic reticulum cisternae. Throughout the DB in M. abbreviatus numerous axonal endings were seen to be in contact with the DB cells exhibiting a synaptic-like structure. The axon terminals contained numerous electron-dense and scanty electron-lucid vesicles. In addition, the DB nerve endings exhibited FMRFamide immunoreactive vesicles. Injection of neural tracer into the DB yielded retrograde labelling of neurons in the metacerebrum lobe of the cerebral ganglia and in the parietal ganglia of the subesophageal ganglia complex. The possibility that some of these retrograde-labelled neurons might be FMRFamide-like neurons that may represent a neural control to the DB in M. abbreviatus is discussed.

Foi analisada a ultraestrutura da glândula reprodutiva corpo dorsal (CD) de Megalobulimus abbreviatus. Imunoistoquímica para microscopia eletrônica foi utilizada para detectar peptídeos relacionados ao tetrapeptídeo FMRFamida nas terminações axonais existentes nessa glândula. Foi utilizada marcação neuronal retrógada com o intuito de localizar os neurônios envolvidos nesta inervação. O CD de M. abbreviatus possui um aspecto uniforme em toda sua extensão, tanto na porção supraesofágica como subesofágica. As células do CD possuem várias características de glândulas esteroidogênicas, tais como a presença de inúmeras gotículas lipídicas, numerosas mitocôndrias com cristas tubulares e cisternas bem desenvolvidas de retículo endoplasmático liso. Por toda a extensão do CD de M. abbreviatus foram encontradas numerosas terminações axonais fazendo contatos estruturalmente semelhantes a sinapses com as células do CD. As terminações axonais continham grande número de vesículas eletrodensas e esparsas vesículas eletrolúcidas. As terminações axonais no CD apresentavam vesículas com conteúdo imunorreativo à FMRFamida. A injeção de traçador neural no CD resultou em marcação retrógrada de neurônios no metacérebro dos gânglios cerebrais e nos gânglios parietais do complexo ganglionar subesofágico de M. abbreviatus. É discutida a possibilidade de que estes neurônios identificados por marcação retrógrada possam representar a via de controle neural do CD de M. abbreviatus, cujo mediador químico seria um neuropeptídeo relacionado à FMRFamida.

Expression of ghrelin and its receptor GHS-R 1A in pancreatic endocrine tumors and the plasma levels of ghrelin and leptin / 中华胰腺病杂志

Objective To study the plasma and intratumoral levels of ghrelin, leptin and their relationship and clinical significance in patients with pancreatic endocrine tumor.Methods Preoperative plasma levels of ghrelin and leptin were detected by ELISA in 11 patients with pancreatic endocrine tumors and 28 normal controls.Expressions of ghrelin and its receptor GHS-R 1A were tested in 11 tumors and 27 paired control tissues by immunohistochemistry staining, and they were correlated with the clinicopathological characteristics.Results The plasma levels of ghrelin was ( 16.0 ± 5.0) pg/ml, which was significantly lower than that in normal controls [ (21.0 ± 2.0) pg/ml, P = 0.047 ].The plasma levels of leptin was (0.34 ±0.03 ) ng/ml, which was not significantly different with that in normal controls [ 0.38 ± 0.04) ng/ml ].There was positive association between plasma levels of leptin and ghrelin (P =0.015 ), but was not associated with clinicopathological parameters.The plasma levels of leptin in control group was positively associated with BMI (P = 0.002), but they were not associated in patients with tumor.The expression rate of ghrelin in tumor tissue was significantly lower than that in control group (64% vs 100%, P = 0.004 ).But the expression rate of GHS-R I A was not significantly different between the two groups.The expression of ghrelin and GHS-R1A in tumor was not significantly associated with clinicopathological parameters.Conclusions The ghrelin and its receptor GHS-R 1A were extensively expressed in pancreatic endocrine tumors, and the serum levels of ghrelin and leptin was changed.

Vasoactive intestinal peptide-secreting-tumor,report of 4 cases / 中华普通外科杂志

Objective To evaluate the diagnosis and treatment of vipoma based on our experience on 4 cases.Method Clinical manifestations,laboratory examination,imaging features,surgical findings,and pathology of 4 patients with vipoma admitted in our hospital from 1991 were discussed.Results Watery diarrhea and hypokalemia were the main clinical manifestations.Hepatic metastasis OCCurred in two patients.Tumor located in the head of the pancreas in one case.Two tumors were shown in the pancreatic body and one tumor was in the pancreatic tail.Resection of tumor and hepatic metastatic lesions with repeat resection of metastases Was performed in 1 patient.Resection of the pancreatic body and tail was done in one patient.Pancreatoduodenectomy Was performed in one patient.Laparotomy only was done in one patient because of invasion of the superior mesenteric vein and duodenum.Conclusion Typical symptoms play an important role in the diagnosis of vipoma.Hepatic metastasis is common.Surgery is the most effective means for treatment.

Mixed Ductal-Endocrine Carcinoma of the Pancreas: A Case Report

Mixed ductal-endocrine carcinoma of the pancreas is composed of ductal and endocrine carcinoma components and each component makes up a significant proportion in the primary tumor as well as in the tumor of metastatic sites. Mixed ductal-endocrine pancreatic carcinoma is exceptionally rare and, to our knowledge, only five cases have been reported in the literature. Recently we experienced a case of mixed ductal-endocrine pancreatic carcinoma with regional lymph node and hepatic metastases in a 63-year-old woman. Here, we report a case of mixed ductal-endocrine pancreatic carcinoma with a review of the literature.

The diagnosis, treatment and clinicopathologic analysis on gastrointestinal neuroendocrine carcinomas / 中国普通外科杂志

Objective To investigate the clinicopathologic features of gastrointestinal neuroendocrine carcinomas. MethodsThe diagnosis and treatment results of 45 cases were studied, and clinicopathologic features and immunohistochemical expressions of NSE, Syn and CgA were detected.ResultsMicroscopically carcinomas were divided into three types: type Ⅰ(25 cases), type Ⅱ(10 cases) and type Ⅲ(10 cases). The histologic categories were correlated with lymph node metastasis significantly( P0.05). The 5-year survival rate for type Ⅰ, type Ⅱ and type Ⅲ was 70%, 65% and 52%, respectively.ConclusionsThe combination of NSE, Syn and CgA immunohistochemical stainnig is necessary for the diagnosis of gastrointestinal neuroendocrine carcinomas. The histologic classification is coincident with the requirement of clinical treatment and prognosis.

Role of LFMPS in the preoperative localization of pancreatic endocrine tumors / 中华消化内镜杂志

Objective To assess the clinical value of low-frequency mini - probe sonography ( LFMPS) in preoperative localization of pancreatic endocrine tumors comparing with other imaging methods. Methods Twenty one cases with suspected pancreatic endocrine tumors were enrolled from June 2000 to June 2002, we compared the diagnostic results of LFMPS, transcutaneous ultrasonography ( US) , helico-computed tomography ( HCT) and magnetic resonance imaging (MRI) with surgical localization and histopathological results by using Fujinon 7. 5 MHz miniature probe and SP-701 ultrasonic system. Results Sixteen pancreatic insulinomas and 1 extra pancreatic VIPoma (vesoactive intestinal polypeptide tumor) were confirmed by surgery and histopathological examination in 17 of the 21 patients, and the rest 4 patients didn't receive surgical procedure because of the negative results in all imaging studies. Among pancreatic lesions, they located on head, body and tail in 9, 3 and 4 cases respectively; the average diameter of all 17 lesions was 2. 02cm. LFMPS correctly localized the tumor in 14 of 17 patients (82. 4% ) while CT in 15 of 17 patients (88. 2% ) , MRI in 12 of 17 patients (70. 6% ) and US in 9 of 17 patients (52. 9% ). Besides, the diagnostic accuracy of LFMPS in detection of small size (

Pancreatic stem cell-like cells in histogenesis of pancreatic endocrine tumors / 中华内分泌代谢杂志

Specimens from 12 cases of pancreatic endocrine tumors were investigated by light and electron microscopy and immunohistochemical staining. Pancreatic stem cell-like cells with special characteristics of pancreatic stem cell were found in the proliferating small ductules and dispersed at the margins of pancreatic endocrine tumors in all the cases studied.