Résumé
Objective To explore the effect of expression of FHL1 on biological character of lung adenocarcinoma cell line A549.Methods Lung adenocarcinoma cell line A549 was transfected by FHL1 mRNA and shRNA lentivirus vector.The stable overexpression of FHL1 in lung adenocarcinoma cell line A549 model and low expression of cell line model were tested by Western blot and RT-PCR method.CCK-8 test,Transwell invasion,flow cytometry and Colony-forming Assay were used to test the proliferation,invasion,apoptosis and anchorage independence growth of the cell line.Results The difference between the stable overexpression of FHL1 group and its low expression group was obvious tested by Western blot and RT-PCR.CCK-8 test showed higher cell proliferation of the low expression group than that of the overexpression group and the blank group (P =0.002 2).Flow cytometry showed that the apoptosis rate of the overexpression group was 50.48 ％,significantly higher than that of the two low expression groups (16.41％,20.12％) and blank group (25.90％,P＜0.001).The invasiveness of the overexpression group was obviously lower than the low expression group and blank group in the Transwell invasion (P＜0.001).Colony-forming Assay showed that the colony number of the overexpression group was 28.7 ± 6.0,significantly less than the two low expression groups (157.0 ± 6.4,150.7 ± 9.5,P＜0.000 1).Conclusions The stable overexpression and low expression of FHL1 in lung cancer cell line A549 models were successfully constructed.The overexpression of FHL1 inhibited the proliferation,invasion and metastasis,and accelerated apoptosis in lung adenocarcinoma cell line A549.
Résumé
Objective To explore the expression of transcriptional regulation factor FHL 1 in pancreatic fibrosis of chronic pancreatitis (CP) and clinical significance.Methods Serum samples from 20 CP patients treated in the Department of Gastroenterology , Nanjing General Hospital and 20 healthy controls in the corresponding period were collected .The level of FHL1 in the serum was detected by ELISA .Twenty male Wistar rats were randomly divided into control group and CP group according to the body weight .The CP rat model was established by injecting dibutyltin dichloride ( DBTC) through tail vein .All the rats were killed at 28 days after injection and the pancreas was collected , which was observed using hematoxylin and eosin ( HE) staining to investigate pathological changes . The expression of FHL1 in pancreas was measured by immunohistochemistry (IHC) and Western blotting (WB).Results The average of serum FHL1 level in two groups were (0.86 ±0.22)g/L and (1.18 ±0.22)g/L, respectively.The level of FHL1 in serum of CP patients was significantly lower than that of healthy cases , and the difference was statistically significant (t=10.54, P=0.001).Damaged pancreatic lobular structure , acinar atrophy and the obvious fibrous hyperplasia were observed in CP rats .The pathological score of abnormal structure , acinar atrophy, fibrosis, and inflammatory cell infiltration in the pancreas of the CP group was (2.2 ±0.4),(2.0 ±0.7),(2.4 ±0.3) and (2.8 ±0.6), respectively, but was all 0 in control group.The difference between two group was statistically significant ( t value =17.24, 9.01, 25.22 and 14.75, respectively, all P<0.001).The expression of FHL1 in the pancreatic tissue of CP group were less than that of normal group , and the difference was statistically significant (IHC:15 798 ±3 247 vs 37 570 ±9 254, t=2.438,P=0.016;WB:0.44 ±0.11 vs 0.89 ±0.03, t=4.289, P=0.002).Conclusions The protein of FHL1 was in low expression in the pancreas of CPs , indicating that FHL1 may participate in the formation of pancreatic fibrosis .