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Objective: To study the antitumor constituents from Chloranthus fortunei. Methods: Various chromatographic techniques and spectroscopic methods were applied to investigate the chemical constituents from C. fortunei, and some of the compounds were screened for their antitumor activities by MTT method. Results: Sixteen compounds were obtained from the whole plants of C. fortunei and identified as rosmarinic acid (1), 2’-hydroxy-4,3’,4’,6’-tetramethoxychalcone (2), flavokawain A (3), cycloshizukaol A (4), atractylenolide III (5), 4β-hydroxy-8,12-epoxyeudesma-7,11-diene-1,6-dione (6), (8α)-6,8-dihydroxycadina-7 (11),10 (15)-dien-12-oic acid γ-lactone (7), curcolonol (8), 11-hydroxyldrim-8,12-en-14-oic acid (9), friedelin (10), isovanillic acid (11), 6β-hydroxystigmast-4-en-3-one (12), 3,4-dihydroxybenzoic acid (13), shikimic acid (14), scopolin (15) and N-acetyltyramine 1-O-β-D-glucoside (16). Compounds 4 and 5 showed weak cytotoxicity with IC50 ranged from 46 to 85 μmol/L. Conclusion: Compounds 2, 10, 11, and 13-15 are obtained from the genus Chloranthus for the first time and compounds 1-3 and 6-16 are isolated from C. fortunei for the first time. Some sesquiterpenoids from C. fortunei exhibited weak antitumor activities.
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En el presente artículo nos proponemos revisar, a la luz de los resultados arrojados por la conclusión de nuestro proyecto de investigación "Presencia y eficacia causal de lo traumático en la cura psicoanalítica de las neurosis: Investigación sobre la complicidad del ser hablante con el azar (tique). Estudio de casos en el Servicio de Clínica de Adultos de la Universidad de Buenos Aires", la hipótesis principal de dicha investigación, planteada en los siguientes términos:"El método psicoanalítico permite despejar la causalidad por elección que interviene en la estructuración, la elaboración y la resolución de la neurosis; esa causalidad se realiza y se manifiesta a través de las incidencias tíquicas de la repetición en sus diversas formas, entre las cuales están lo traumático en su relato historizado, el ocasionamiento de los síntomas, y sus actualizaciones transferenciales". Además, consignaremos el modo en que hemos podido aislar, formalizar y observar algunas variables intervinientes en el corpus de casos seleccionado como muestra, proveniente del Servicio de Clínica de Adultos de la Facultad de Psicología, que funciona en la ciudad de Avellaneda.
In this article we propose to check, in the light of the results produced by the conclusion of our project of investigation "Presence and causal eficacy of the trauma in psychoanalytic treatment of neurosis: Investigation on the complicity of the speaking being with the fortune (tyche). Study of cases in the Service of Adults' Clinic of the University of Buenos Aires", the principal hypothesis of the above mentioned investigation, raised in the following terms: "The psychoanalytic method allows to clear the causality by choice that intervenes in the structure, production and resolution of the neurosis; this causality is realized and demonstrated across the tychique incidents of the repetition in its diverse forms, between which are the traumatic tale and its transferencial updates ". In addition, we will record the way in which we could have isolated, formalize and to observe some intervener variables in the corpus of cases selected as sample, from the Service of Adults' Clinic of the Faculty of Psychology, in Avellaneda's city.
Sujets)
Humains , Théorie psychanalytique , Troubles névrotiques , CausalitéRésumé
Objective: To study the genetic diversity and genetic relationship of Istais indigotica from different habitas by ISSR-PCR marker technique, so as to provide the reference for its germplasm identification and breeding. Methods: Twenty-three samples of I. indigotica and its variation types from the different habitas of China were studied by ISSR-PCR markers. The Jaccard coefficient was worked out by NTSYS-pc software, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA). Results: Ten ISSR primers generated 109 loci of which 88 loci were polymorphic. The average percentage of polymorphic bands (PPB) was 80.7%. The germplasm of I. indigotica had the plentiful genetic diversity. In the cluster dendrogram, the studied samples were clustered into three groups, the first one included majority samples, the second was the variation type with hair, and the third one was the farthermost, which was separated at the coefficient of 0.62. Conclusion: ISSR method is suitable for the germplasm identification and genetic diversity analysis of I. indigotica, thus providing a biological theoretical foundation for its cultivation and breeding.
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O estudo do desenvolvimento foliar é de grande importância para a avaliação do crescimento e desenvolvimento das plantas. O objetivo deste trabalho foi quantificar as consequências dos diferentes níveis do manejo de água de irrigação sobre o crescimento foliar e o número de folhas do Kalanchoe. O delineamento experimental utilizado foi inteiramente casualizado, com oito tratamentos de irrigação (15; 13; 11; 9; 8; 6; 4 e 2mm) e dez repetições. A área foliar das plantas foi calculada por meio do produto do comprimento e da largura de cada folha, multiplicada pelo coeficiente de 1,1134. Os resultados mostraram que as lâminas de irrigação entre 6 e 9mm apresentaram maior crescimento foliar e maior número de folhas por planta. A máxima eficiência técnica foi encontrada com lâminas de 6,68 e 8,59mm, para área foliar e número de folhas por planta, respectivamente. Em condições de déficit hídrico, o Kalanchoe reduz expressivamente sua elongação foliar transversal em relação à longitudinal.
The research about the leaf development is very important to evaluate the plants growth and development. The objective of this research was to verify the different level consequences about the irrigation water manner in the leaf growth and the number of the Kalanchoe leaves. The experimental search used was completely randomized, with eight treatments, they consisted in 15; 13; 11; 9; 8; 6; 4 e 2mm irrigation in ten repetitions. The plants leaf area was calculated by the area of each leaf, multiplied by the coefficient of 1.1134. The results showed that the irrigation length between 6 and 9mm showed a bigger leaf growth and more leaves in each plant; the biggest technical efficiency was found with 6.68 and 8.59mm length, to each leaf area and the leaves number in leaf/plant, respectively. In a water condition deficit the Kalanchoe reduces a lot the leaf transversal growth related to the longitudinal.
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Objective To study the effect and mechanism of serum carrying Fortune's drynaria rhizome chomocor on proliferation, differentiation, and mineralization of osteoblast of new-born SD rats. Methods (1) Preparation of serum carrying Fortune's drynaria rhizome chromocor: Fortune's drynaria rhizome chromocor was administered once everyday for continuous 12 days to 12 months old female SD rats. Blood was obtained from aorta. (2) Creation of the experiment model; Isolated the osteoblast from the neonatal SD rats' calvaria by using enzyme digestion twice, subcultured. The third passage of cultured osteoblast was chosen as the experiment model. (3) Index; ①The proliferation capacity of osteoblast: detected by method of MTT. ②The differentiation activity of osteoblast: detected by the alkaline phosphatase assay (PNPP) and the amount of ostecalcin (radioimmunoassay). ③The mineralization activity of osteoblast: detected by counting mineral deposites under 40× microscope after chinalizarin staining. Results Serum carrying Fortune's drynaria rhizome chromocor stimulated proliferation of osteoblast of new-bom SD rats in vitro, promoted the excretion of alkaline phosphatase and osteocalsin, and increased the formation of the mineral deposites. Conclusions Fortune's drynaria rhizome chromocor can stimulate proliferation, differentiation, and mineralization of osteoblasts and promote the bone formation.
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Objective:To discuss the effect of shuanghuangbu delivery device on the regeneration of the experimental periodontium.Methods:The second incisor and the canine of four beagle dogs were randomly divided into the experimental group and the control group.Shuanghuangbu was applied to periodontal defect in the experimental group and no disposition to periodontal defect in the control group.The specimens were respectively obtained in one month and three months after operation and then observed.Furthermore,the height of new bone and cementum formation and the length of junctional epithelium were measured in the two groups.Results:The heights of new bone and cementum regeneration in the experimental group were extremely bigger than those in the control group on one month and three months postoperatively ( P 0.05).Conclusion:Shuanghuangbu can obviously promote the new bone and cementum formation,restrain the migration of junctional epithelium and enhance the regeneration of periodontal tissue.