Résumé
Objective: To investigate the chemical constituents from the fruits of Rhododendron molle. Methods: Compounds were isolated and purified by a series of methods including silica gel, ODS column, Sephadex LH-20, semi-preparative HPLC, recrystallization, and so on. Their structures were identified by analysis of physico-chemical propertiesand spectroscopic technique. Results: Fifteen known compounds were isolated and their structures were elucidated as myricetin (1), dihydromyricetin (2), gallocatechin (3), quercetin-3′-O-glycoside (4), catechin (5), epicatechin (6), taxifolin 3′-O-glucopyranoside (7), proanthocyanidin A-2 (8), dehydroicatechin A (9), quercetin-3-O-α-arabinoside (10), phlorizin (11), lyoniresinol 3-O-rhamnopyranoside (12), 2,6- dimethoxy-4-hydroxyphenol-1-O-glucopyranoside (13), 2,4,6-trihydroxy acetophenone-2-O-glucopyranoside (14), and 5′-β- glucopyranosyloxy-jasmonic acid (15). Conclusion: Compounds 1—4, 7—9, and 13—15 are obtained from R. molle for the first time. Compounds 5, 6, and 10—12 are obtained from the fruits of this plant for the first time, of which compounds 1—11 are flavonoids.
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Objective To establish an HPLC fingerprint method of Sanlejiang Oral Liquid (SOL) and determine the contents of its main components, combining with clustering analysis for quality consistency evaluation of different batches, so as to provide a reference for the quality control. Methods Welchrom C18 (250 mm × 4.6 mm, 5 μm) column was adopted, the mobile phase consisted of 0.1% phosphoric acid water-methanol with gradient elution at the flow rate of 1.0 mL/min, and the detection wavelength was 270 nm, the column temperature was 25 ℃. HPLC fingerprint of SOL was established and determination method of gallic acid, gallocatechin, epicatechin, corilagin, and ellagic acid were studied methodologically. Results The fingerprint chromatography included 22 mutual peaks. At the same time, the 10 batches of SOL fingerprints were analyzed by similarity software, the similarity among the batches was more than 0.95. Based on the tetention time of master compounds, five components [gallic acid (peak 3), gallocatechin (peak 8), epicatechin (peak 15), corilagin (peak 16), and ellagic acid (peak 21)] were identified and quantified. The contents of gallic acid, gallocatechin, epicatechin, corilagin, and ellagic acid in 10 batches of Sanlejiang Oral Liquid were 5.743 2- 7.538 0, 0.492 9-0.847 1, 0.529 7-0.804 8, 0.937 5-1.756 5, and 0.352 7-0.554 5 mg/mL, respectively. Conclusion The established method is simple in good separation and reproducibility, achieving the qualitative and quantitative research for SOL, and thus can provide a reference for the standard and evaluation of quality of SOL.
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AIM To establish a quantitative analysis of multi-components by single marker(QAMS) method for the content determination of six catechins in Xinnaojian Capsules (Tablets) (tea extract).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Shimadzu Wonda Cract ODS-2 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of 0.5％ acetic acid (A)-acetonitrile (B) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.With epigallocatechin gallate as an internal standard,the relative correction factors of epigallocatechin,catechin,epicatechin,gallocatechin gallate and epicatechin gallate were calculated,from which the content determination was made.RESULTS Six constituents showed good linear relationships within their own ranges (r ≥ 0.999 8),whose average recoveries were 96.00％-98.47％ with the RSDs of 2.09％-2.91％.The results obtained by QAMS method approximated those obtained by external standard method.CONCLUSION This simple and reliable method can be used for the quality control of Xinnaojian Capsules (Tablets).
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Objective: To explore the mechanism of the compatibility of Mongolian medicine Sendeng-4. Methods: Sendeng-4 was composed of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction with Gardeniae Fructus, Toosendan Fructus, and Chebulae Fructus with a mass ratio of 5:3:1:1. BEH C18 column (50 mm×2.1 mm, 1.7 μm) was used as the chromatographic column, water (0.1% formic acid)-methanol were used as mobile phase for the gradient elution, and the ultra performance liquid chromatography coupled with mass spectrometry (UPLC-MS), principle component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the chemical constituent changes of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and its co-decoction. Results: The significant differences between the group of Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium and Sendeng-4 were observed in PCA and OPLS-DA model. There were significant differences among eight chemical compounds after compatibility (P<0.05). The relative contents of catechin, (-)-gallocatechin, dihydromyricetin, quercetin decreased, xanthocerasic acid, 3β-hydroxytirucalla-7,24-dien-21-oic acid, 3-oxotirucalla-7,24-dien-21-oic acid, and rutin were increased. Conclusion: Maybe the anti-inflammatory and anti-rheumatic activity of Sendeng-4 have the relationship with the content changes of some index component in Xanthoceratis Sorbifoliae Lignum seu Ramulus et Folium after compatibility.
Résumé
The pharmacokinetic profile of gallocatechin-7-gallate (J10688) was studied in rats after intravenous administration. Male and female Sprague-Dawley (SD) rats received 1, 3, and 10 mg/kg (i.v.) of J10688 and plasma drug concentrations were determined by a high performance liquid chromatography-mass spectrometry (LC-MS) method. The pharmacokinetic software Data Analysis System (Version 3.0) was used to calculate the pharmacokinetic parameters. For different i.v. doses of J10688, the mean peak plasma concentration (C 0) values ranged from 11.26 to 50.82 mg/L, and mean area under the concentration-time curve (AUC0-t ) values ranged from 1.75 to 11.80 (mg·h/L). J10688 lacked dose-dependent pharmacokinetic properties within doses between 1 and 10 mg/kg, based on the power model. The method developed in this study was sensitive, precise, and stable. The pharmacokinetic properties of J10688 in SD rats were shown to have rapid distribution and clearance values. These pharmacokinetic results may contribute to an improved understanding of the pharmacological actions of J10688.