Cytotoxic function of gamma delta (ϒ/δ) T cells against pamidronate- treated cervical cancer cells.

The cytotoxic function of polyclonal expanded ϒ/δ T cells against pamidronate-treated cervical cancer cells in vitro and in vivo were determined. The ϒ/δ T cells were isolated and purified from PBMCs by using miniMACS and were later treated with 10 μM pamidronate. The expansion of ϒ/δ T cells was 15 times more than the non-stimulated cells. Among the expanded ϒ/δ T cells, 47% were Vϒ9/Vδ2 T cells with a purity of 87%. Analyzing the cytotoxic function of ϒ/δ T cells against 3 cervical cancer cells in vitro by LDH cytotoxicity test revealed that the killing efficacy increased if the cervical cancer cells (HeLa, SiHa and CaSki) were pretreated with pamidronate. The presence of CD107 on ϒ/δ T cells indicated the degranulation of perforin and granzyme pathway is one of the mechanisms used by the ϒ/δ T cells to kill cancer cells. The killing ability of ϒ/δ T cells against cancer cells in vivo was preliminary assessed by using mouse baring HeLa cells. The results demonstrated that ϒ/δ T cells induce apoptosis in tumor cells. Our study supports the usefulness of ϒ/δ T cells in future development of immunotherapy for cervical cancer.

Cytotoxic effects of human γδT cells on hepatocellular carcinoma cells in vitro / 中国基层医药

Objective To investigate the cytotoxic effects of human γδT cells on hepatocellular carcinoma cells in vitro.Methods Normal human PBMC were activated by IPP and rhIL-2.The expansion of γδT cells were analyzed by flow cytometry.The concentration of interferon gamma in the supernatants was detected by ELISA.The cytotoxicity of activated lymphocytes against hepatocellular carcinoma cells were measured by the lactate dehydrogenase release assay in vitro.Results After PBMC activated by IPP and rhIL-2 for 10 days,the proportion of γδT cells were expanded from (4.34±1.79)% to (55.65±6.88 )%.The secretion of interferon gamma expanded significantly after PBMC stimulated by IPP,and reached its peak value at the seventh day.The secretion of interferon gamma is lower if it has no IPP.The activated lymphocytes stimulated by IPP were detected to have cytotoxicity in vitro against human hepatocellular carcinoma ceils.Conclusion The high proportion of γδT cells can be gained in human PBMC activated by IPP,PBMC stimulated by IPP can secret interferon gamma effectively and the activated γδT cells have high cytotoxlcity in vitro against human hepatocellular carcinoma cells.