Résumé
Objective To perform qualitative and quantitative analysis on the volatile components in roots, rhizomes, leaves, and flowers of Asari Radix et Rhizoma derived from Asarum insigne. Methods The volatile components were analyzed by HS-GC-MS, and the relative percentage content of each component was calculated with peak area normalization method. Results There were 58 components separated from four parts of A. insigne, including 27 common components in different parts. The principal constituents was trans-β-farnesene, safrole, and asaricin. Their contents were different in four parts. Especially the contents of safrole in rhizomes, leaves, and flowers were up to 34%, 22%, and 21%; The safrole in rhizomes was over twice higher than that in roots (12%). Because safrole was extremely poisonous, the rhizomes, leaves, and flowers should be used carefully. Conclusion The volatile components in A. insigne can be detected by HS-GC-MS simply and quickly. The research can be helpful for development and quality evaluation of A. insigne.
Résumé
Objective: To establish a rapid analytical method for volatile components in Tibetan medicine Heracleum millefolium and to determine the volatile components from its roots, stems, leaves, and flower parts, respectively. Methods: Headspace sampling incorporation with gas-chromatography-mass spectrum (HS-GC-MS) determination was introduced to analyze the powder directly. Static headspace equilibration was performed at 100℃ for 40 min, and 1 mL of the headspace gas was injected in split mode of 10:1. The split inlet temperature was 260℃. The carrier gas was He at a constant flow rate of 1.8 mL/min. The column oven temperature was initially set at 50℃ for 2 min, then increased to 100℃ at 2℃/min, held for 6 min, then increased to 300℃ at 10℃/min and held for 2 min. The GC/MS interface temperature was maintained at 280℃. The solvent delay time was 3 min (to bypass the solvent peak). The volatile components were confirmed by NIST11.L database, and volatile organic compounds from roots, stems, leaves, and flower parts were compared. Results: The types of compounds in the roots, stems, leaves, and flowers of H. millefolium are mainly aldehyde, benzene, alcohols, and alkene. Octanal, hexanal, and γ-terpinene are the main components in the roots and stems. While o-isopropyltoluene and terpinolene are the main components in the leaves and flowers. Conclusion: HS-GC-MS method is easy, simple, and feasible, and can be widely used in other Chinese materia medica samples for analysis of volatile components.