Résumé
AIM To establish HPLC fingerprints of Geranium strictipes R.Kunthand to perform pattern recognition.METHODS The analysis of 70％ methanol extract of G.strictipes was performed on a 25℃ thermostatic ZORBAX SB-C18 column(250 mm× 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water in a gradient elution manner,and the detection wavelength was set at 254 nm.The results were analyzed by cluster analysis and principal component analysis.RESULTS There were thirteen common peaks in the HPLC fingerprints of twenty-one batches of samples with the similarities of more than 0.894.Twenty-one batches of samples were divided into two categories.The cumulative contribution rate of the first principal component was 31.067％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.strictipes.
Résumé
Objective To investigate the inhibitory activity of neuraminidase (NA) and the chemical constituents of the active fraction of the extract from Geranium strictipes, and further investigate the anti-NA activity of the components by molecular docking technique and NA inhibition test. Methods G. strictipes was extracted by petroleum ether, ethanol, ethyl acetate and water, and four extracts were obtained. The inhibition activity of NA was determined by fluorescence method and the inhibition rate was calculated. UPLC-Q-Exactive was used to rapidly identify and identify the chemical constituents in the most active sites. Using MOE 2014, seven main components were inserted to NA. And the NA inhibitory activity of these seven major components was determined by fluorescence method, and the inhibition rate was calculated. Results The results showed that the ethyl acetate was the most active with the IC50 value of 137.31 μg/mL. Moreover, a total of 20 chemical constituents were identified by UPLC-Q-Exactive, including 3 phenolic acids, 2 flavonoids, and 16 tannins. Among them, seven chemical constituents were identified accurately with the standard and 13 compounds were reported for the first time in the plant. The results of molecular docking showed that pentagalloylglucose, ellagic acid and protocatechuic acid had better antagonistic effect. Further NA inhibition test showed that all the seven compounds had inhibitory activity, especially for the ellagic acid and pentagalloylglucose which had the best effect and IC50 were 426.67 and 440.28 μmol/L, respectively. Conclusion The extract from G. strictipes have the inhibitory activity of NA, and the ellagic acid and pentagalloylglucose are main active compenents.
Résumé
Objeetive:To screen the different extracts from Geranium strictipes with anticaries activity,and to study the effect of the active extract on the production of acid and polysaccharides of oral cariogenic bacteria.Methods:Chlorhexidine and Galla Chinensis were used as the positive controls.Tube double dilution method was used to study the extracts of Geranium strictipes on the growth of Streptococcus mutans (S.mutans) and Actinomyce viscosus (A.viscosus).First,the activity of 5 extracts fom Geranium strictipes against S.mutans,A.viscosus was detected.Their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained.The effects of 4 lower concentrations(＜ MIC) of the active extract on the production of oral bacterial acid polysaccharides were examined.Results:The effect of n-butanol extraction part of Geranium strictipes was the strongest among all the extracts against the two kinds of bacteria in the inhibition of bacteria growth,bacterial acid production and polysaccharides production,but weaker than that of Chlorhexidine and Galla Chinensis.Conclusion:N-butanol extraction part of Geranium strictipes may be a natural and effective anti-caries agent.