Clinical outcomes of epithelial ingrowth following laser in situ keratomileusis with mechanical debridement and compressed heating air flow: a case series / Resultado visual do tratamento do crescimento epitelial após laser in situ ceratomileuse através da remoção mecânica e aplicação de ar comprimido aquecido: série de casos

ABSTRACT Purpose: To describe the clinical outcomes of manual scraping of epithelial ingrowth followed by compressed heating air flow after laser in situ keratomileusis (LASIK). Methods: We underwent a retrospective, noncomparative, and interventional case series. Twenty eyes of 17 patients were included in this study. Each patient with a history of LASIK underwent epithelial removal with mechanical debridement followed by compressed heating air flow. Our primary outcome was the recurrence of epithelial ingrowth after 3 months of follow-up, while our secondary outcomes were uncorrected distance visual acuity, corrected distance visual acuity, and complications after surgery. Results: Ten patients (58.8%) were male, and eight eyes of seven (41.2%) patients underwent primary LASIK surgery, while12 eyes of 10 patients had flap-lift retreatment LASIK; sixteen eyes (80.0%) underwent mechanical microkeratome LASIK and four (20.0%) underwent femtosecond laser-assisted LASIK. Mean age at surgical removal of epithelial ingrowth was 37.0 years ± 9.3 years (range 24 to 55 years). There was recurrence of ingrowth in two eyes (10%) after 3 months of follow-up. The mean corrected distance visual acuity of patients before surgery was 0.07 ± 0.09 logMAR, and after the last follow-up was 0.02 ± 0.04 logMAR (p=0.06). The odds ratio of presenting with epithelial ingrowth after LASIK enhancement compared to primary LASIK was 29.41. Conclusion: Manual scraping followed by compressed heating air flow is a safe and effective treatment of clinically significant epithelial ingrowth after LASIK. At the last follow-up, no eye lost any line in corrected distance visual acuity.

RESUMO Objetivo: Descrever os resultados clínicos do tratamento do crescimento epitelial através da técnica de remoção manual seguido da utilização de um compressor de ar comprimido aquecido após a cirurgia de laser in situ keratomileusis (LASIK). Métodos: Vinte olhos de 17 pacientes foram incluídos no estudo. Cada paciente havia sido submetido a cirurgia de LASIK com presença de crescimento epitelial e foi submetido a tratamento cirúrgico para sua retirada. O objetivo primário foi identificar a presença de crescimento epitelial recorrente ao final de 3 meses de seguimento. Os objetivos secundários foram as medidas de acuidade visual sem correção, acuidade visual com correção, e complicações pós-operatórias. Resultados: Dez pacientes (58,8%) eram homens e 7 mulheres. Oito olhos de sete (41,2%) pacientes apresentavam cirurgia de LASIK primária e 12 olhos de 10 pacientes tinham cirurgia de LASIK com retratamento; dezesseis olhos (80%) utilizaram microcerátomo manual e quatro (20%) laser de femtosegundo. A média de idade no momento da cirurgia de remoção do epitélio era de 37,0 anos ± 9,3 (DP) (variando de 24 a 55 anos). Ocorreu recidiva do crescimento epithelial em dois olhos (10%) após 3 meses de seguimento. A acuidade visual sem correção antes da cirurgia era de 0,07 ± 0,09 logMAR, e após a cirurgia passou para 0,02 ± 0,04 logMAR (p=0,06). A chance (odds ration) de aparecimento do crescimento epithelial após uma reoperação de LASIK é 29,41 vezes maior do que no LASIK primário. Conclusão: A técnica de remoção epitelial manual seguida da utilização de ar comprimido aquecido é segura e efetiva no tratamento do crescimento epitelial após LASIK. Ao final do último acompanhamento, nenhum olho apresentou perda de linhas de visão.

Influencia de la malnutrición fetal en el crecimiento y desarrollo craneofacial

Introducción: La malnutrición fetal incide de forma negativa en el crecimiento y la maduración, afectando las estructuras craneofaciales en el feto. Objetivo: Determinar los efectos de la malnutrición fetal por defecto en el crecimiento y desarrollo craneofacial en niños. Métodos: Se realizó un estudio analítico de casos y controles, en el área de salud José Martí del municipio Camagüey, desde enero de 2018 hasta diciembre de 2020. El universo estuvo constituido por niños de 6-11 años de edad, pertenecientes al área salud y la muestra quedó conformada por 40 niños, 20 en el grupo de estudio y 20 controles. El grupo de estudio se conformó por niños que presentaron bajo peso al nacer por defecto y tuvieron alteraciones en el crecimiento del complejo craneofacial y el grupo control por niños que no presentaron alteraciones en el crecimiento del complejo craneofacial y que al nacimiento se encontraban normopeso. A todos los niños se les efectuaron mediciones antropométricas craneofaciales. Resultados: La medida de la circunferencia cefálica al nacer en los controles masculinos superó en 0,78 cm al sexo femenino y entre los malnutridos por defecto, los masculinos lograron 1,57 cm más que los femeninos. En el diámetro bicigomático hubo una fuerte asociación estadística y el riesgo relativo indicó que la incidencia del bajo peso al nacer fue mayor que en los controles (p <0,05). Conclusiones: Los niños con bajo peso al nacer presentan menor crecimiento y desarrollo craneofacial según las variables antropométricas.

Introduction: Fetal malnutrition impacts in a negative way the growth and development, thus affecting the craniofacial structures of the fetus. Objective: To determine the effects of fetal malnutrition by defect on craniofacial growth and development in children. Methods: An analytical case and control study was carried out in Jose Martí health area of Camagüey, from January 2018 to December 2020. A sample of 40 was selected from the totality of the children between 6 and 11 years old who belong to the referred health area, to be divided into 20 cases and the same number in the control group. The study group was composed of those low birth weight who present craniofacial abnormalities while control group included the children without craniofacial abnormalities and normal weight at birth. Anthropometric craniofacial measurements were performed on all the sample. Results: The head circumference at birth in male children of control group was 0.78 cm higher than that on female sex. Among those low birth weight, male children exhibited 1.57 cm higher than females. Regarding the bizygomatic diameter there was a strong statistic association and the relative risk indicated a higher incidence on the study group compared to the control group (p <0.05). Conclusions: Low birth weight children show lower craniofacial growth and development, with higher impact on females according to anthropometric variables.

Indicadores de normalidad del patrón de maduración de la deglución, su evaluación en la práctica médica / Evaluating normal pattern of the mature swallowing in medical practice

RESUMEN Introducción: En Villa Clara se establecieron indicadores de normalidad del patrón de maduración de la deglución, pero aún no han sido evaluados. Objetivo: Evaluar los indicadores de normalidad del patrón de maduración de la deglución. Métodos: Se realizó un estudio descriptivo, transversal, en 107 niños en Santa Clara, desde septiembre del 2016 hasta noviembre del 2017. Se utilizó una guía de observación de la deglución para evaluar los indicadores. Resultados: El comportamiento de estos fue adecuado en 30, de los 36 niños. En el grupo de edad de 2 años y 4 meses hasta 2 años y 10 meses se observaron maduras en más de un 70 por ciento las variables: ruidos al tragar, escape de los alimentos y posición de la lengua; a los 2 años y 11 meses hasta los 3 años y 5 meses: movimientos del cuello, contracción de los maseteros y residuos de los alimentos (ante la ingesta de sólidos); y a los 3 años y 6 meses: contracción del mentón y contracción de los labios. Conclusiones: Los indicadores de normalidad del patrón de maduración de la deglución fueron evaluados como efectivos.

ABSTRACT Introduction: indicators of normal pattern of the mature swallowing were established in Villa Clara, but they have not yet been evaluated. Objective: to evaluate the indicators of normal pattern of the mature swallowing. Methods: a descriptive cross-sectional study was carried out in 107 children from Santa Clara, between September 2016 and November 2017. An observation guide of swallowing was used to evaluate the indicators. Results: the manifestation of these indicators was evaluated as adequate in 30 of the 36 children. Swallowing sounds, escape of food and tongue position were observed to be mature, in more than 70 percent, in the age group between 2 years and 4 months and 2 years and 10 months; neck movements, contraction of masseter muscles and food residues (before eating solids) at 2 years and 11 months up to 3 years and 5 months; as well as contraction of chin and lips at 3 years and 6 months. Conclusions: the indicators of normal pattern of the mature swallowing were evaluated as effective.

Testicular volume and Tanner stage: determinant factors for testicular torsion

ABSTRACT Objective To assess testicular volumes and sexual maturation in patients with testicular torsion. Methods A retrospective analysis of consecutively treated patients with testicular torsion between 2016 and 2018. Age, pubic hair staging (Tanner), and by ultrasonography, volume of the unaffected testis (in cubic centimeters) were evaluated either immediately before surgery or at the first postoperative visit. Patients with previous testicular disease, such as cryptorchidism, or with no records of testicular volume were excluded. The analysis included descriptive statistics and Bayesian regression. Results We treated 149 patients during the study period, and 141 (94.6%, median age 17.3 years) met the inclusion criteria. Median testicular volume was 13.0cm3 (interquartile range of 10.5-15.2), with similar right and left volumes (12.9cm3versus 13.3cm3; p=0.94). Sixty-five (46.1%) patients were Tanner stage IV, 17 (12.1%) stage III, and 59 (41.8%) stage V. Conclusion In this study, we were able to estimate volumes of testicular torsion, which aggregated around late puberty values (13.0cm3 for the whole dataset, 12.2cm3 for patients <25 years), suggesting that testicular hypermobility, due to congenital anatomical abnormalities, remains quiescent until the organ reaches a critical volume, after which torsion becomes possible. These findings provide a tentative explanation for the disease's age distribution.

Crecimiento transversal del maxilar con uso de placa ortopédica prequirúrgica en pacientes con fisura labiopalatina / Transverse growth of the maxilla with the use of a presurgical orthopaedic plate in patients with cleft lip and palate

RESUMEN Introducción: la ortopedia prequirúrgica en el tratamiento de la fisura labiopalatina constituye una pauta terapéutica controversial. Los investigadores que están a favor plantean que favorece el cierre de la fisura lo cual facilita la cirugía, y otros apoyan el criterio de limitar el crecimiento maxilar y provocar su colapso. Objetivo: determinar los efectos morfológicos en el crecimiento transversal del maxilar durante el período de uso de la placa ortopédica prequirúrgica en niños con fisura labiopalatina atendidos en el Hospital Provincial Universitario «José Luis Miranda» de Villa Clara, en el período de marzo de 2015 a junio de 2019. Métodos: se realizó un estudio de intervención longitudinal. La muestra estuvo conformada por 34 niños previo consentimiento de sus padres o tutores. La información se obtuvo a través de: la entrevista, el examen clínico y mediciones; se aplicaron pruebas estadísticas como el test de Wilcoxon y el estadístico exacto de Fisher. Resultados: en la muestra estudiada predominaron los pacientes del sexo masculino y la fisura transforamen. Con el uso de la placa ortopédica prequirúrgica el maxilar mostró un crecimiento uniforme con un incremento de 5,6 mm de la distancia intercanina y de 5,2 mm de la distancia postgingival desde el nacimiento hasta la cirugía del paladar. La media de crecimiento entre el nacimiento y la cirugía del labio fue de 2,7 y 2,6 mm para la distancia intercanina y postgingival, respectivamente. Conclusiones: la ortopedia prequirúrgica favorece el crecimiento transversal del maxilar con un incremento uniforme de su ancho anterior y posterior.

ABSTRACT Introduction: presurgical orthopaedics in the treatment of cleft lip and palate constitutes a controversial therapeutic guideline. Researchers who are in favour state that it favours fissure closure which facilitates surgery, and others support the criterion of limiting maxillary growth and causing its collapse. Objective: to determine morphological effects on the transverse growth of the maxilla during the period of use of the presurgical orthopaedic plate in children with cleft lip and palate treated at "José Luis Miranda" Provincial University Hospital in Villa Clara from March 2015 to June 2019. Methods: a longitudinal intervention study was carried out. The sample consisted of 34 children with the prior consent of their parents or guardians. The information was obtained through interview, clinical examination and measurements; Wilcoxon's test and Fisher's exact test were applied as statistical tests. Results: male patients and transforamen fissure predominated in the studied sample. The maxilla showed, with the use of the presurgical orthopaedic plate, a uniform growth with an increase of 5.6 mm in the intercanine distance and a 5.2 mm increase in the post-gingival distance from birth to palate surgery. The mean growth between birth and lip surgery was 2.7 and 2.6 mm for intercanine and post-gingival distance, respectively. Conclusions: presurgical orthopaedics favours transverse growth of the maxilla with a uniform increase in its anterior and posterior width.

Morfología cefalométrica de la sínfisis del mentón en individuos jóvenes de la ciudad de Quito-Ecuador / Cephalometric morphology of chin symphysis in young individuals from the city of Quito-Ecuador

Resumen Introducción: La sínfisis del mentón aporta equilibrio, simetría y armonía al rostro. Por ser una estructura anatómica mandibular muy importante y altamente modificable en cirugía estética, se considera indispensable conocer su morfología y así obtener una correcta interacción en el diagnóstico, pronóstico y terapéutica médico-odontológica. Objetivo: Determinar la morfología sínfisis del mentón en jóvenes adultos de la ciudad de Quito- Ecuador, año 2019, mediante la identificación de las longitudes sagitales y coronales. Metodología: Este estudio tuvo un enfoque cuantitativo, tipo descriptivo-observacional, con temporalidad retrospectiva. Se calibraron, analizaron y trazaron 384 radiografías digitales laterales de adultos jóvenes de la ciudad de Quito-Ecuador, año 2019 en el software AutoCAD-2019. Resultados: Existió una elevada frecuencia de la forma geométrica cuadrada (60%) con respecto a la rectangular (40%), adicionalmente se identificó que la figura cuadrada es más frecuente en individuos de sexo femenino y sujetos clase I (56%) y II (70%), además de todos los patrones de crecimiento vertical hipodivergentes (58%), normodivergentes (64%) e hiperdivergentes (57%). Conclusiones: La forma cuadrada de la sínfisis del mentón es mayor que la forma rectangular. Por la originalidad de los resultados, se sugiere realizar nuevos estudios que correlacionen la morfología mentoniana con las diferentes estructuras cráneo-maxilofaciales.

Abstract Introduction: The chin symphysis contributes to the balance, symmetry and harmony of the face. Since it is an important anatomical jaw structure and highly modifiable in aesthetic surgery, it is considered crucial to know its morphology and, thus, obtain a correct interaction in the diagnosis, prognosis and medical-dental therapy. Objective: To determine the symphysis morphology of the chin in young adults in the city of Quito, Ecuador, in the year 2019, through the identification of sagittal and coronal lengths. Methodology: This study had a quantitative approach, descriptive-observational type, with retrospective temporality. We calibrated, analyzed and plotted 384 lateral digital radiographs of young adults from the city of Quito-Ecuador, year 2019 in the software AutoCAD-2019. Results: There was a high frequency of the square geometric shape (60%) in comparison to the rectangular one (40%). Additionally, it was identified that the square figure is more frequent in female individuals and class I (56%) and II (70%) subjects, in addition to all the hypodivergent (58%), normodivergent (64%) and hyperdivergent (57%) vertical growth patterns. Conclusions: The square shape of the chin symphysis is larger than the rectangular shape. Due to the originality of the results, it is suggested to carry out new studies that correlate chin morphology with the different cranio-maxillofacial structures.

Testes respiratórios não invasivos para o diagnóstico de SBID e intolerância à lactose em pacientes com câncer colorretal e gástrico em tratamento quimioterápico / Noninvasive breath tests for diagnosis of sibo and lactose intolerance in patients on chemotherapy treatment for colorectal and gastric câncer

ABSTRACT BACKGROUND: Worldwide, colorectal cancer (CRC) and gastric cancer (GC) are the third and the fifth most prevalent, respectively. Diarrhea is a common symptom in patients on chemotherapy or radiotherapy treatment and can reduce treatment tolerance. Surgical resections and chemotherapy change the intestinal microbiota that can lead to lactose intolerance, small intestinal bacterial overgrowth (SIBO). OBJECTIVE: The aim of the study was to evaluate the frequency of diarrhea in patients with CRC and GC on chemotherapy with SIBO or intolerance of lactose. METHODS: This is a descriptive and observational study with patients of both sexes, over 18 years old, in treatment in the Gastro-Oncology outpatient clinic of the Federal University of São Paulo. Patients with a confirmed diagnosis of CRC or GC during chemotherapy treatment were included. To detect bacterial overgrowth and lactose intolerance, breath hydrogen test with lactulose and lactose was done. Number and aspects of the evacuations and toxicity degree were collected. For the nutritional assessment, weight and height were performed to calculate the BMI. and the Patient Generated Subjective Global Assessment (PG-SGA). RESULTS: A total of 33 patients were included, 29 with CRC and 3 with GC. Most of them were male (57.57%), mean age of 60.03±10.01 years and in chemotherapy with fluoropyrimidine and oxaliplatin (54.5%). Diarrhea was present in 57.6% and 30.3% had toxicity grade 2. According to the BMI, 78.9% were eutrophics, obese or overweight, but according to PG-SGA, 84.9% had moderate or severe nutritional risk grade. Between patients, 45% had lactose intolerance and 9% SIBO. Diarrhea grade 2-3 was observed in 66.6% of patients with SIBO and 66.7% of that with lactose intolerance. No statistical difference was observed between patients with SIBO or lactose intolerance and grade of diarrhea. CONCLUSION: Diarrhea was a frequent symptom in chemotherapy patients with gastric or colorectal cancer independent of the presence of SIBO or lactose intolerance. Surgery and chemotherapy treatment impacted in the intestinal habit of patients. Diagnosis of other causes of diarrhea may contribute to a better tolerance to treatment and quality of life.

RESUMO CONTEXTO: Mundialmente, o câncer colorretal (CCR) e gástrico (CG) são a terceira e a quinta causa de câncer mais prevalente, respectivamente. A diarreia é um sintoma comum entre os pacientes em quimioterapia ou radioterapia e pode reduzir a tolerância ao tratamento. Quimioterapia e ressecções cirúrgicas causam alterações da microbiota intestinal que podem levar a intolerância à lactose e ao supercrescimento bacteriano do intestino delgado (SBID). OBJETIVO: Avaliar a presença de diarreia nos pacientes com câncer colorretal e gástrico em quimioterapia e a presença de SBID ou intolerância à lactose. MÉTODOS: Foi realizado um estudo descritivo, observacional com pacientes ambulatoriais de ambos os sexos, maiores de 18 anos, em tratamento no ambulatório de gastro-oncologia da Universidade Federal de São Paulo. Foram incluídos pacientes com diagnóstico confirmado de CCR ou CG durante tratamento quimioterápico. Para detectar supercrescimento bacteriano e intolerância à lactose, foram realizados testes respiratórios com lactulose e lactose respectivamente. Número, aspecto das evacuações e grau de toxicidade foram coletados. Para a avaliação nutricional foram aferidos peso e altura para cálculo do IMC e para avaliação do risco nutricional foi realizada a avaliação subjetiva global produzida pelo próprio paciente (ASG-PPP). RESULTADOS: Foram incluídos 33 pacientes, 29 com CCR e 3 com CG. A maioria era do sexo masculino (57,5%) com média de idade 60,03±10,01 anos e em tratamento quimioterápico com fluoropirimidina e oxaliplatina (54,5%). Diarreia foi relatada por 57,6% dos pacientes sendo em 30% grau 2. Pelo IMC, 78,9% apresentavam eutrofia, sobrepeso ou obesidade grau 1, mas pela ASG-PPP 84,9 apresentavam risco nutricional moderado ou severo. Entre os pacientes 9% apresentavam SBID e 45% intolerância à lactose. Diarreia grau 2-3 foi observada em 66,6% daqueles pacientes com SBID e 66,7% dos com intolerância à lactose. Não encontramos diferenças estatísticas entre os pacientes com SBID ou intolerância à lactose e intensidade de diarreia. CONCLUSÃO: Diarreia foi um sintoma frequente entre os pacientes com câncer gástrico ou colorretal em quimioterapia independente da presença de SBID ou intolerância à lactose. Cirurgia e quimioterapia impactaram no hábito intestinal dos pacientes. O diagnóstico de outras causas de diarreia pode contribuir para a melhor tolerância do tratamento e qualidade de vida.

Potential pregnancy days lost: an innovative measure of gestational age / Dias potenciais de gravidez perdidos: uma medida inovadora da idade gestacional

ABSTRACT In Brazil, the excess of interventions that anticipate childbirth, such as cesarean sections and labor inductions, has resulted in the shortening of pregnancy, with negative consequences on maternal-infant outcomes. This commentary presents a novel way to measure gestational age: the continuous variable "Potential pregnancy days lost." Using data from the Live Birth Information System (SINASC), we counted the missing days between the period until childbirth and the average duration of pregnancy (280 days), or the lost weeks. This measure can be used as an outcome variable (socioeconomic-demographic characteristics of the mother, type of childbirth, financing, etc.) or exposure variable (for neonatal, infant, and maternal outcomes). The indicator can be used in municipal and national cohorts and intervention studies to analyze hospitals and regions. We discuss the limits and scope of gestational age measures and, given their inaccuracies, the importance of studying their trends.

RESUMO No Brasil, o excesso de intervenções que antecipam o parto, como cesarianas e induções, tem resultado no encurtamento da gestação, com consequências negativas nos desfechos materno-infantis. Neste comentário apresentamos uma medida inovadora da idade gestacional que estamos desenvolvendo: a variável contínua "dias potenciais de gravidez perdidos". Utilizando dados do Sistema de Informações de Nascidos Vivos, contamos, quando possível, os dias faltantes entre o período até o nascimento e a duração média da gestação (280 dias), ou então as semanas perdidas. Essa medida pode ser usada como variável desfecho (de características socioeconômico-demográficos da mãe, do tipo de parto, de financiamento etc.) ou de exposição (para desfechos neonatais, infantis e maternos). O indicador pode ser usado em coortes municipais e nacionais e em estudos de intervenção, para analisar hospitais e territórios. Discutimos os limites e alcances das medidas de idade gestacional e, diante de suas imprecisões, a importância de estudar suas tendências.

Influence of strategic points in the dispersion of Aedes aegypti in infested areas / Influência de pontos estratégicos na dispersão de Aedes aegypti em áreas infestadas

ABSTRACT OBJECTIVE: To evaluate whether sites with large amount of potential breeding sites for immature forms of Aedes aegypti, called strategic points, influence in the active vector's dispersion into properties in their surroundings. METHODS: We selected four areas in the municipality of Campinas, three of them with strategic points classified as high, moderate, and low risk according to infestation and a control area, without strategic points. Between October 2015 and September 2016, we monthly installed oviposition traps and evaluated the infestation by Ae. aegypti in all properties of each selected area. To verify if there was vector dispersion from each strategic point, based on its location, we investigated the formation of clusters with excess of eggs or larvae or pupae containers, using the Gi spatial statistics. RESULTS: The amount of eggs collected in the ovitraps and the number of positive containers for Ae. aegypti did not show clusters of high values concerning its distance from the strategic point. Both presented random distribution not spatially associated with the positioning of strategic points in the area. CONCLUSIONS: Strategic points are not confirmed as responsible for the vector's dispersion for properties in their surroundings. We highlight the importance of reviewing the current strategy of the vector control program in Brazil, seeking a balance from the technical, operational, and economic point of view, without disregarding the role of strategic points as major producers of mosquitoes and their importance in the dissemination of arboviruses in periods of transmission.

RESUMO OBJETIVO: Avaliar se locais com grande quantidade de potenciais criadouros de formas imaturas de Aedes aegypti, denominados pontos estratégicos, influenciam a dispersão ativa do vetor aos imóveis no seu entorno. MÉTODOS: Foram selecionadas quatro áreas no município de Campinas, três delas com pontos estratégicos classificados como alto, médio e baixo risco segundo a infestação e uma área controle, sem ponto estratégico. Entre outubro de 2015 e setembro de 2016, instalaram-se mensalmente armadilhas de oviposição e avaliou-se a infestação por Ae. aegypti em todos os imóveis de cada área selecionada. Para verificar se houve dispersão do vetor a partir de cada ponto estratégico, com base em sua localização, investigou-se a formação de aglomerados com excesso de ovos ou de recipientes com larvas ou pupas, utilizando a estatística espacial Gi. RESULTADOS: o número de ovos coletados nas ovitrampas e o número de recipientes positivos para Ae. aegypti não apresentaram aglomerados de altos valores relativos à sua distância do ponto estratégico. Ambos apresentaram distribuição aleatória não associada espacialmente com o posicionamento dos pontos estratégicos na área. CONCLUSÕES: Pontos estratégicos não se confirmaram como responsáveis pela dispersão do vetor para os imóveis no seu entorno. Destaca-se a importância de rever a estratégia atual do programa de controle de vetores do Brasil, buscando um equilíbrio do ponto de vista técnico, operacional e econômico, sem desconsiderar o papel dos pontos estratégicos como grandes produtores de mosquitos e sua importância na disseminação de arboviroses em momentos de transmissão.

Caracterização de variáveis clínicas e do desenvolvimento motor de recém-nascidos prematuros / Characterization of clinical variables and neuromotor development of preterm infants

A Organização Mundial de Saúde considera prematuro aquele nascido com idade gestacional entre 20 a 37 semanas. Com os avanços tecnológicos existentes nas Unidades de Terapia Intensiva Neonatal, tem-se maior sobrevida destes, que apresentam desenvolvimento neuromotor inferior aos recém-nascidos a termo. Este estudo objetivou caracterizar o desenvolvimento motor e variáveis clínicas de prematuros nascidos em um hospital público. Trata- se de um estudo misto. A pontuação na Escala Motora Infantil de Alberta, peso ao nascimento, idade gestacional, tempo de internação na Unidade de Terapia Intensiva Neonatal e Apgar de 1o e 5o minuto de prematuros com idade corrigida entre 0 e 18 meses foram coletadas dos prontuários, entre maio de 2013 a abril de 2014. Para análise estatística, utilizou-se o teste do Qui-Quadrado (nível de significância p<0,05). Foram incluídos 267 prematuros, com mediana de idade gestacional de 32(30 - 33) semanas, de tempo de internação de 35 (25 - 58,5) dias e de peso médio de 1430 (11-75 - 1690) gramas. Foi encontrado em 10,49% das crianças o Apgar no 5o minuto inferior a 7. Verificou-se que 36,89% dos prematuros entre 0 e 5 meses, 39,6% entre 06 e 12 meses e 23,5% entre 13 e 18 meses estavam com riscos ou atrasos motores evidentes. Não foi observada correlação entre o Apgar do 5o minuto e o desenvolvimento motor. A maioria das crianças entre 06 e 12 meses apresentou risco para atrasos motores, justificando a importância do seguimento de prematuros em serviços de referência, mesmo após o primeiro ano de vida.

The World Health Organization considers preterm those born with a gestational age between 20 and 37 weeks. With the technological advances in Neonatal Intensive Care Units, we have seen a higher survival rate among preterms, who present lower neuromotor development than full term newborns. The aim of this study was to characterize the motor development and clinical variables of preterm infants born in a public hospital. It is a mixed study. The score on the Alberta Infant Motor Scale, birth weight, gestational age, time of stay in the Neonatal Intensive Care Unit, and the 1st and 5th minute Apgar of preterm infants with ages between 0 and 18 months corrected age, were collected from their files between May 2013 and April 2014. For statistical analysis, the Chisquare test (significance level of p < 0.05) was used. A total of 267 premature babies were included, with median gestational age of 32 (30-33) weeks, duration of stay of 35 (25-58.5) days, and mean weight of 1,430 (1,175-1,690) grams. A 5th minute Apgar score below 7 was found in 10.49% of the children. It was found that 36.89% of the infants between 0 and 5 months, 39.6% between 6 and 12 months, and 23.5% between 13 and 18 months of age were at risk of or had evident motor delay. No correlation was observed between the 5th minute Apgar and motor development. Most of the infants between 6 and 12 months were at risk for motor delay, justifying the importance of the follow-up for premature infants in specialized services even after the first year of life.

Correção óptica e evolução da hipermetropia / Optical correction and evolution of hipermetropy

Resumo Objetivo: Comparar as alterações da refração e da biometria ocular na população infantil hipermetrópica com e sem correção óptica total. Métodos: Realizou-se estudo prospectivo longitudinal não randomizado em 41 pacientes com hipermetropia, entre 3 e 6 dioptrias ou/e com esotropia acomodativa pura nos ambulatórios do Hospital Geral Universitário e Oftalmocenter Santa Rosa, com idade inicial entre 4 e 6 anos. Os pacientes foram divididos em dois grupos, em que o Grupo 1 compôs-se pelos pacientes hipermétropes que não necessitavam usar sua correção óptica ou poderiam usá-la parcialmente, e o Grupo 2 por pacientes com esotropia acomodativa pura e pelos hipermétropes que necessitavam usar toda sua correção óptica. Os pacientes submeteram-se a exame oftalmológico completo, incluindo refração objetiva em autorrefrator com cicloplegia, biometria óptica e topografia corneana em uma medida inicial e outra 3 anos mais tarde. Comparou-se a refração e parâmetros biométricos com teste T student. Resultados: A média da idade inicial foi de 5,23 ± 0,81 e 5,36 ± 0,74 anos, a refração inicial foi +3,99 ± 0,92 e +4,27 ± 0,85 D, o diâmetro anteroposterior do globo ocular foi de 21,42 ± 0,84 e 21,22 ± 0,86 mm, e a ceratometria foi de 42,55 ± 1,24 e 42,39 ± 1,22 D, para os Grupos 1 e 2, respectivamente. Em relação à refração, houve redução significativa do poder esférico no Grupo 1, em 3 anos; e não houve no Grupo 2 (p<0,05). Com relação ao diâmetro anteroposterior do globo ocular, ocorreu aumento significativo no Grupo 1 e não houve no Grupo 2 (p<0,05 ). Não se verificou diferença significativa na comparação das ceratometrias em 3 anos nos Grupos 1 e 2. Conclusão: Estes dados permitiram concluir que a correção total da hipermetropia pode prejudicar a emetropização natural em crianças.

Abstract Objective: To compare changes in refraction and ocular biometric parameters in hyperopic children with and without full optical correction. Methods: Non-randomized prospecting study with 41 subjects (21 males and 20 females) aged 4 to 6 years with accommodative esotropia and or hyperopia between 3 to 6 diopters, select in Hospital Geral Universitário and Oftalmocenter Santa Rosa. The patients were divided in two groups: group 1 for hyperopic patients that did not need to use optical correction or could use partial correction, and group 2 for patients with accommodative esotropia or hyperopia who needed to use full optical correction all the time. The patients were examined to a complete ophthalmological examination, including objective cycloplegic refraction with auto refractometer, optical biometry and corneal topography, in baseline measurements and 3 years after that. Refraction and ocular biometric parameters were compared using T student test. Results: The mean initial age was 5.23 ± 0.81 and 5.36 ± 0.74 years; the initial refractive error in average was +3.99 ± 0.92 e +4.27 ± 0.85 D, the initial axial length was 21.42 ± 0.84 and 21.22 ± 0.86 mm, and initial keratometry was 42.55 ± 1.24 e 42.39 ± 1.22 D for group 1 and 2, respectively. In relation to refractive error, there was a significant decrease in group 1 and there was not in group 2 (p < 0.05). In relation to axial length, there was significant increase in group1 and there was not in group 2 (p<0.05). The 3-year comparison showed no statistically significant differences in keratometry for both groups. Conclusion: This study suggests that full optical correction of hyperopia may inhibit natural emmetropization during early and late childhood.

THE INFLUENCE OF PASSIVE TOBACCO EXPOSURE AND PHYSICAL EXERCISE ON BONE TISSUE OF YOUNG RATS / INFLUÊNCIA DO TABAGISMO PASSIVO E DO EXERCÍCIO FÍSICO NO TECIDO ÓSSEO DE RATOS JOVENS

ABSTRACT Objective: The objective of this study is to investigate the effect of passive smoking during pregnancy and associated with swimming on bone area growth, bone mineral density (BMD), and bone mineral content (BMC). Methods: The offspring was grouped by control matrices (G1) and passive smokers (G2). The offspring was regrouped in eight subgroups, with exposure to smoking (2x/day) and physical exercise (1 session/day), respecting the group of matrices in: sedentary control (G1CS and G2CS), swimming control (G1CN and G2CN), sedentary passive smoker (G1FS and G2FS), and passive smoker swimmer (G1FN and G2FN). The area, BMD and BMC were measured by the tibia and femur and analyzed by densitometer. The results were analyzed by One-Way ANOVA test with Tukey post-test, with a significance level of 5%. Results: In the tibia BMC study, a better rate was observed in G2CN group when compared to G1CS, G1CN and G1FN (p ≤ 0.023). When assessing BMD in the femur, a higher density ratio was observed in G1FS group when compared to G2CS, G2CN, G2FS and G2FN (p<0.008). In the tibia study, the animals of the G1FS group had higher rates when compared to G2CS and G2FN groups (p≤0.007). Conclusions: The model of male offspring exposed to passive smoking during fetal development showed a strong decrease in the analyzed parameters. Level of Evidence I, Randomized High Quality Clinical Trial With or Without Statistically Significant Difference, But Narrow Confidence Intervals.

RESUMO Objetivo: O objetivo desse estudo é investigar o efeito do tabagismo passivo durante a prenhez e associado à natação no crescimento de área óssea, densidade mineral óssea (DMO) e conteúdo mineral ósseo (CMO). Métodos: Os filhotes foram gerados por matrizes controle (G1) e tabagistas (G2). Os filhotes foram reagrupados em oito subgrupos, com exposição ao tabagismo (2x/dia) e realização de exercício físico (1 sessão/dia), respeitando o grupo de matrizes em: controle sedentário (G1CS e G2CS), controle natação (G1CN e G2CN), tabagista passivo sedentário (G1FS e G2FS) e tabagista passivo natação (G1FN e G2FN). A área, a DMO e o CMO foram aferidos pela tíbia e pelo fêmur e analisados pelo densitômetro. Os resultados foram analisados pelo teste de ANOVA One-Way, com pós-teste de Tukey, com nível de significância de 5%. Resultados: No estudo do CMO da tíbia, foi observada taxa melhor no grupo G2CN quando comparada aos filhotes dos grupos G1CS, G1CN e G1FN (p ≤ 0,023). Na averiguação da DMO no fêmur, foi observada maior taxa de densidade no grupo G1FS quando confrontado aos grupos G2CS, G2CN, G2FS e G2FN (p ≤ 0,008). Já no estudo da tíbia, os animais do grupo G1FS apresentaram maiores taxas quando comparados aos grupos G2CS e G2FN (p≤0,007). Conclusões: O modelo de filhotes machos expostos ao tabagismo passivo durante o desenvolvimento fetal apresentou uma forte diminuição dos parâmetros analisados. Nível de Evidência I, Estudo Clínico Randomizado de Alta Qualidade Com ou Sem Diferença Estatisticamente Significante, Mas Com Intervalos de Confiança Estreitos.

Characteristics of iris vessels exposure and its relevant factors in normal full-term neonates / 中华眼底病杂志

Objective To observe the rate of iris vessels exposure and analyze its relevant factors in normal full-term neonates.Methods A retrospective study.1855 normal full term neonates,including 947 boys and 908 girls,were enrolled.The mean gestational age (GA) was (38.84±1.10) weeks and mean birth weight (BW) was (3 396.52±402.08) g.There were 1235 neonates from normal term vaginal delivery,402 cases of cesarean delivery and 218 cases of forceps delivery.All neonates were examined with hand-held portable slit lamp biomicroscopy within 1 to 3 days after birth by two trained ophthalmologist respectively.Iris vessels exposure was defined as radial red blood vessels along iris fibers.Infants were divided into iris vessels exposure group and iris vessels unexposed group according to the findings of slit lamp biomicroscopy.78 infants with iris vessels exposure were followed up for 42 days after birth till the iris vessels can't be seen under microscope.The differences between the two groups were compared for gender,mode of delivery (MOD),GA,BW and body length (BL).Multiple logistic regressions were used to determine the factors related to iris vessels exposure.Results There were 298 neonates with iris vessels exposure among 1855 neonates and the rate was 16.1％.1557 neonates (83.9％) had unexposed iris vessels.There were no different in gender (x2=0.551) and MOD (x2=3.036) between iris vessels exposure group and unexposed group (P＞0.05),while the differences in GA (x2=47.216),BW (t=4.603) and BL (t=3.936) between the two groups were statistically significant (P=0.000).Multiple logistic regression analysis revealed that only GA (β=-0.291,odds ratio=0.747,95％ confidence interval:0.656-0.85 1,P=0.000) was correlated to iris vessels exposure significantly.The iris vessels couldn't be seen in 77 of 78 infants with iris vessels exposure when followed up to 42 days.Conclusions The iris vessels exposure in normal full-term neonates is frequently observed.There is a significant inverse correlation between GA and iris vessels exposure.

Aedes aegypti breeding site in an underground rainwater reservoir: a warning / Criadouro de Aedes aegypti em reservatório subterrâneo de água da chuva: um alerta

ABSTRACT We describe the discovery of Aedes aegypti underground breeding site in the Pinheiros neighborhood of São Paulo, SP, during an entomological survey program performed in 2016. Even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. A detailed investigation allowed for the detection of Ae. aegypti in an underground reservoir used for rainwater storage. After the implementation of protection screens in the accesses, the presence of the vector was no longer detected. In this study, we discuss the frequent use of this type of reservoir structure and its risk for mosquito production.

RESUMO Descreve-se o encontro de criadouro subterrâneo de Aedes aegypti no bairro de Pinheiros, São Paulo, SP, ocorrido durante um programa de levantamento entomológico, realizado em 2016. Mesmo com intensa vigilância e controle vetorial, grande quantidade de mosquitos estava presente nessa área. Investigação minuciosa permitiu a detecção de Ae. aegypti em reservatório subterrâneo para armazenamento de água pluvial. Após a implantação de telas de proteção nos acessos, não foi mais detectada a presença do vetor. O uso frequente desse tipo de estrutura e o seu risco para a produção de mosquitos é discutido.

Morfología cefalométrica de la sínfisis del mentón en individuos jóvenes de la ciudad de Quito-Ecuador

Introducción: La sínfisis del mentón aporta equilibrio, simetría y armonía al rostro. Por ser una estructura anatómica mandibular muy importante y altamente modificable en cirugía estética, se considera indispensable conocer su morfología y así obtener una correcta interacción en el diagnóstico, pronóstico y terapéutica médico-odontológica. Objetivo: Determinar la morfología sínfisis del mentón en jóvenes adultos de la ciudad de Quito- Ecuador, año 2019, mediante la identificación de las longitudes sagitales y coronales. Metodología: Este estudio tuvo un enfoque cuantitativo, tipo descriptivo-observacional, con temporalidad retrospectiva. Se calibraron, analizaron y trazaron 384 radiografías digitales laterales de adultos jóvenes de la ciudad de Quito-Ecuador, año 2019 en el software AutoCAD-2019. Resultados: Existió una elevada frecuencia de la forma geométrica cuadrada (60%) con respecto a la rectangular (40%), adicionalmente se identificó que la figura cuadrada es más frecuente en individuos de sexo femenino y sujetos clase I (56%) y II (70%), además de todos los patrones de crecimiento vertical hipodivergentes (58%), normodivergentes (64%) e hiperdivergentes (57%). Conclusiones: La forma cuadrada de la sínfisis del mentón es mayor que la forma rectangular. Por la originalidad de los resultados, se sugiere realizar nuevos estudios que correlacionen la morfología mentoniana con las diferentes estructuras cráneo-maxilofaciales.

Introduction: The chin symphysis contributes to the balance, symmetry and harmony of the face. Since it is an important anatomical jaw structure and highly modifiable in aesthetic surgery, it is considered crucial to know its morphology and, thus, obtain a correct interaction in the diagnosis, prognosis and medical-dental therapy. Objective: To determine the symphysis morphology of the chin in young adults in the city of Quito, Ecuador, in the year 2019, through the identification of sagittal and coronal lengths. Methodology: This study had a quantitative approach, descriptive-observational type, with retrospective temporality. We calibrated, analyzed and plotted 384 lateral digital radiographs of young adults from the city of Quito-Ecuador, year 2019 in the software AutoCAD-2019. Results: There was a high frequency of the square geometric shape (60%) in comparison to the rectangular one (40%). Additionally, it was identified that the square figure is more frequent in female individuals and class I (56%) and II (70%) subjects, in addition to all the hypodivergent (58%), normodivergent (64%) and hyperdivergent (57%) vertical growth patterns. Conclusions: The square shape of the chin symphysis is larger than the rectangular shape. Due to the originality of the results, it is suggested to carry out new studies that correlate chin morphology with the different cranio-maxillofacial structures.

Response properties of neurons and synaptic plasticity during pre-critical period of visual development in mouse visual cortex / 中华实验眼科杂志

Background The visual development is completed during the critical period in human and mammals.However,the critical period is not the initial of receiving visual experience.It is known that before the onset of critical period in mammals,such as mouse,there is an earlier stage for visual development,the pre-critical period.The research of response characteristics of the visual cortical neurons and the synaptic plasticity in the pre-critical period is still in the exploratory stage.Objective The study aimed to preliminarily investigate the response properties of neurons and synaptic plasticity in mouse visual cortex during the pre-critical period.Methods Fortyeight postnatal day 13-17 C57BL/6J mice were used for in vivo whole-cell recordings and in vitro brain slice wholecell recordings.In vivo whole-cell recordings were done in anesthetized mice.Moving bars in different directions were produced and controlled by a Matlab program.Cell recordings were obtained at the depth of layer Ⅳ of visual cortex.Step current stimuli under current clamp were given to measure the membrane response properties of neurons.Optimal visual stimuli were given to measure the in vivo largest responses of membrane potentials.In vitro experiments were performed after in vivo experiments.All cells were given current step stimuli to measure the membrane response properties of neurons.Different intensities of white-matter-to-layer-Ⅳpathway stimulation were given to measure the evoked response properties.All cells from 48 mice were randomized into 4 groups according to different stimulus training modes,including low frequency stimulation (LFS),high frequency theta-burst stimulation (TBS),pre-post synaptic timing stimulation (pre-post TS) and post-pre synaptic timing stimulation (post-pre TS).Under the voltage clamp of-70 mV,excitatory postsynaptic currents (EPSCs) before and after training were recorded to measure the plastic changes of excitatory synaptic connections.pClamp 10 was used for the pre-analysis of data and Matlab 2008a was used for statistical analysis.The use and care of the animals followed the Statement for the Use of Animals in Ophthalmic and Vision Research.Results Thirty-nine cells and 48 cells were successfully recorded in the in vivo and in vitro experiments,respectively.The steady-state average number of action potentials (APs) were (1.01 ± 0.03)/sweep and (1.01 ±0.05)/sweep,the AP thresholds were (-40.2 ± 3.2) mV and (-39.6 ±2.0) mV,and the threshold step current levels were (126.7 ± 17.4) pA and (129.6 ± 17.5) pA in the in vivo and in vitro recordings,respectively,with no significant differences between them (APs:t =0.512,P =0.610;AP thresholds:t =-1.074,P =0.286;current levels:t =-0.776,P =0.440).Under the optimal visual or pathway stimulation,the average peak response of membrane potentials was (7.3 ±4.3)mV and (6.4±2.8)mV with rarely evoked APs in the in vivo and in vitro experiments,respectively,with no significant difference between them (t =1.234,P =0.221).Under the in vitro recording,the EPSCs before LFS were [(138.1 ±51.9)pA],which was significantly higher than that after LFS [(76.1 ± 34.8)pA] (t=4.437,P=0.001),but no significant differences were seen in EPSCs before and after TBS (t=-0.756,P=0.466).The EPSCs before and after pre-post TS were (122.4±62.2)pA and (78.5±46.7)pA,and those before and after post-pre TS were (131.9 ±48.0) pA and (74.3 ± 30.7) pA,showing significant differences between them (pre-post TS:t =3.558,P =0.004;post-pre TS:t =4.283,P =0.001).Conclusions The construction of fundamental neural circuits in layer Ⅳ of mouse visual cortex is completed during pre-critical period.However,the membrane responsive capability of neurons and the synaptic connections are in an immature state,and the evoked responses to visual pathway inputs are basically subthreshold.The strength of synaptic connections is depressed with low frequency stimulation or pre-post/post-pre synaptic timing stimulation,and kept unchanged with high frequency stimulation.The development of visual neural system of PSP in mouse presents different characteristics from CP.

Hair Zinc Level Analysis and Correlative Micronutrients in Children Presenting with Malnutrition and Poor Growth / 대한소아소화기영양학회지

PURPOSE: Zinc deficiency can induce serious clinical problems in the gastrointestinal (GI) system and immune system and can affect growth and development. It is more severe in younger patients. Chronic zinc deficiency is reflected more precisely in hair than in serum. We studied hair zinc levels and other hair and serum micronutrients in chronic malnourished children to identify which micronutrients are affected or correlated with the other ones. METHODS: Hair mineral analyses were performed in 56 children (age, 1-15 years) presenting with malnutrition, poor growth, poor appetite, anorexia, with/without other GI symptoms (diarrhea, abdominal pain, constipation) from August 2012 to March 2015. Biochemical studies for macronutrients and major micronutrients were also conducted. RESULTS: Hair zinc deficiency was diagnosed in 88%, and serum zinc deficiency was diagnosed in 55% of the children. There was no statistical correlation between serum and tissue zinc level. Hair zinc levels were highly correlated with serum vitamin D (r=−0.479, p=0.001), which also showed correlation with hair levels of magnesium and calcium. (r=0.564, 0.339, p=0.001, 0.011). Hair calcium level was correlated with serum pre-albumin (r=0.423, p=0.001). These correlations may explain the phenomenon that the major clinical manifestation of zinc deficiency is poor body growth. Clinical symptoms were resolved in most children after zinc supplementation. CONCLUSION: Hair zinc and mineral analyses are useful as a therapeutic guide in the clinical investigation of children with malnutrition and poor growth.

Development of the Developmental Support Competency Scale for Nurses Caring for Preterm Infants

PURPOSE: Developmental care has been recognized as a very important component for the development and health promotion of preterm infants. However, research on how to assess developmental nursing competency has not been studied as expected. This study was done to develop and evaluate a new scale to measure nursing competency for developmental support of preterm infants. METHODS: Concept analysis was done with using the Hybrid model of Schwartz-Barcott and Kim (2000), from which a preliminary new scale (30 items) was developed. To test the validity and reliability of the new scale being developed, data were collected from 122 NICU nurses at 4 hospitals in 3 cities in the Republic of Korea, from December, 2014 to March, 2015. RESULTS: The final version of the Developmental Support Competency Scale for Nurses (DSCS-N) caring for premature infants was a 4-point Likert type scale, consisting of 19 items, and categorized as 6 factors, explaining 62.5% of the total variance. Each of the factors were named as follows; ‘environmental support’ (4 items), ‘parental support’ (3 items), ‘interaction’ (3 items), ‘critical thinking’ (3 items), ‘professional development’ (3 items), and ‘partnership’ (3 items). The Cronbach's α coefficient for the scale was .83 and the reliability of the subscales ranged from .60~.76. CONCLUSION: The psychometric evaluation of the new scale demonstrated an acceptable validity and reliability. Findings indicate that the DSCS-N can be used as the tool to test the effect of educational programs for nurses and contribute to advance developmental care for preterm infants.

Bloqueio do receptor AT1 da Angiotensina II reduz o número de folículos antrais em ratas com obesidade induzida por dieta de cafeteria / Blockade of AT1 receptor of Angiotensin II reduces the number of antral follicles in female rats with obesity induced by cafeteria diet

OBJETIVO: Avaliar o desenvolvimento folicular em ratas Wistar com obesidade induzida por dieta de cafeteria (DCAF) submetidas à administração de losartan (LOS), um antagonista do receptor AT1 da Angiotensina II. MÉTODOS: Aos 21 dias de vida, as ratas foram separadas aleatoriamente em dois grupos: controle (CTL), que recebeu ração padrão, e cafeteria (CAF), que recebeu a DCAF, altamente palatável e calórica. Aos 70 dias de vida, início da idade reprodutiva, animais do grupo CAF foram subdivididos em dois grupos (n=15/grupo): CAF, que recebeu água, e CAF+LOS, que recebeu 30 mg/kg de peso corporal (PC) de LOS por gavagem durante 30 dias. O grupo CTL também recebeu água por gavagem. Aos 100 dias de vida foi realizada a eutanásia dos animais e o PC e das gorduras retroperitoneal, perigonadal e subcutânea foi avaliado. Os ovários direitos foram retirados para contagem do número dos diferentes tipos de folículos ovarianos. As concentrações plasmáticas dos hormônios folículo-estimulantes (FSH), luteinizante (LH), prolactina (PRL) e progesterona foram avaliadas. Os resultados foram expressos como média±erro padrão da média. Para análise estatística, foi utilizado one-way ANOVA, seguido pelo pós-teste de Newman-Keuls (p<0,05). RESULTADOS: O PC e das gorduras, assim como o número de folículos antrais, foi elevado no grupo CAF em relação ao CTL. Todavia, as concentrações de FSH e LH foram mais baixas entre os animais CAF. A administração de LOS reduziu o PC e das gorduras retroperitoneal e subcutânea, bem como o número de folículos antrais. O tratamento com LOS atenuou a redução das concentrações de FSH e de LH. As concentrações de progesterona e PRL foram semelhantes entre os grupos estudados. CONCLUSÃO: O uso de LOS pode favorecer o desenvolvimento folicular em fêmeas obesas e pode possibilitar sua utilização como fármaco coadjuvante no tratamento da infertilidade associada à obesidade. .

PURPOSE: To evaluate the follicular development of female Wistar rats with obesity induced by the cafeteria diet, submitted to the administration of losartan (LOS), an antagonist of the AT1 receptor of Angiotensin II. METHODS: At weaning (21 days of age), female Wistar rats were randomly divided, into two groups: control (CTL) that received standard chow and cafeteria (CAF) that received a cafeteria diet, a highly palatable and highly caloric diet. At 70 days of age, at the beginning of the reproductive age, animals of the CAF group were subdivided into two groups (n=15/group): CAF, that received water, and CAF+LOS, that received LOS for 30 days. The CTL group also received water by gavage. At 100 days of age, the animals were euthanized and body weight (BW) as well as the retroperitoneal, perigonadal and subcutaneous fat weights were analyzed. The right ovaries were isolated for counting the number of primary, secondary, antral and mature follicles. Plasma levels of FSH, LH, prolactin and progesterone hormones were analyzed. The results were expressed as mean±standard error of the mean. Data were analyzed statistically by one-way ANOVA followed by the Newman-Keuls post-test (p<0.05). RESULTS: BW and fat weight, as well as the number of antral follicles, were higher in the CAF group compared to the CTL group. However, FSH and LH levels were lower in CAF animals compared to CTL animals. LOS administration attenuated the reduction of FSH and LH levels. Progesterone and PRL levels were similar among groups. CONCLUSION: LOS could improve follicular development in obese females and could be used as an adjunctive drug in the treatment of infertility associated with obesity. .