Résumé
Objective To observe HA14-1 sensitizes Lewis lung carcinoma in mice to cyclophosphamide (CTX) and to explore its possible mechanism. Methods Forty Lewis lung carcinoma model mice were randomly divided into 4 groups:normal saline group,CTX group, HA14-1 group,CTX+HA14-1 group. After the treatment of 7 days,all of the mice were killed on the 22nd day of tumor inoculation. The tumor volume growth curve of each group was described;tumor inhibition rate was caculatued;Bcl-2,Bax,Caspase-9 protein expression levels before and after the treatment were determined by immunohistnehemistry. Results Compared to the normal saline group,HA14-1 group had no significant effect on inhibiting tumor volume,and the tumor volume in HA14-1 group increased less slowly than that of CTX group, HA14-1 group and CTX+HA14-1 group. Compared to the normal saline group, the tumor inhibition rate of HA14-1 group had no significant increase (P> 0.05),while that of CTX group and CTX + HA14-1 group increased significantly (P< 0.05);compared to the CTX group,the tumor inhibition rate of CTX + HA14-1 group increased significantly (P < 0.05). Bcl-2 protein expression levels in CTX group,HA14-1 group and CTX+HA14-1 group were lower than that in the normal saline groups compared to CTX group,Bcl-2 protein expression of CTX + HA14-1 group reduced significantly. Compared to the normal saline group,the expression levels of Bax and Caspase-9 protein in CTX group, HA14-1 group and CTX+HA14-1 group increased significantly (P< 0.05);compared to CTX group,CTX + HA14-1 group increased more significantly (P < 0.05). Conclusion HA14-1 might enhance the efficiency of CTX chemotherapy via inhibiting the expression of Bcl-2, increasing the expression of Bax and caspase-9 and promoting tumor cell apoptosis.
Résumé
AIM: To observe the effects of valproic acid(VPA) and HA14-1 on Bcl-2 overexpressed leukemia cells in vitro and in vivo.METHODS:(1) Cells were divided into control group,HA14-1 group,VPA group and HA14-1+VPA group.The apoptotic rate,the mean fluorescent index(MFI) of Bcl-2,the levels of caspase 3,8 and 9 were detected with FCM.(2) 24 h after transplantation with BALL-1,the NOD/SCID mice were divided into 4 groups as(1),then the survival time was compared.The expression of CD19 in the peripheral blood cells,bone marrows,livers,spleens and lungs of each group was detected.RESULTS:(1) The apoptotic rate in HA14-1+VPA group was(76.5?6.9)%,this was significantly elevated compared to the data in other groups(P