Résumé
Objective: To investigate the clinical significance and predictive value of human papillomavirus (HPV) 16/18 E6 protein in the different cervical intraepithelial neoplasia. Methods: The expression of HPV16/18 E6 in 10 normal cervical tissues, 33 cervical intraepithelial neoplasia I (CIN I ), 31 CIN II-III, 30 cervical cancers was detected by immunohistochemistry, explored the expression difference and the relationship with the clinicopathological characteristics of cervical cancer and the prognosis of different CIN. Results: The positive expression rates of HPV16/18 E6 in normal cervical tissues, CIN I, CIN II-III and cervical cancer group were up-regulated (χ2=19.82, P=0.000). HPV16/18 E6 increased positive expression rates in the low grade and the big size tumors of cervical cancer tissues were detected (P=0.033, P=0.011). There were positive correlations between the overexpression and the pathological grade, tumor size, poor prognosis of cervical cancers respectively (r=0.456, P=0.011; r=0.578, P=0.000; r=0.645, P=0.000). The sensitivity, specificity, and diagnostic accuracy rates of HPV16/18 E6 positive expression to the progression of CIN I, CIN II-III and cervical cancer were respectively 100.00%, 62.50%, 43.75%; 96.77%, 91.30%, 92.86%; 96.97%, 83.87%, 66.67%. Conclusion: HPV16/18 E6 overexpression plays an important role in the generation, development and the poor prognosis of cervical cancer. HPV16/18 E6 has a good predictive value for the prognosis and hierarchical management of cervical diseases.
Résumé
Objective·To investigate the clinical significance and predictive value of human papillomavirus (HPV) 16/18 E6 protein in the different cervical intraepithelial neoplasia. Methods·The expression of HPV16/18 E6 in 10 normal cervical tissues, 33 cervical intraepithelial neoplasiaⅠ (CINⅠ ), 31 CINⅡ- Ⅲ, 30 cervical cancers was detected by immunohistochemistry, explored the expression difference and the relationship with the clinicopathological characteristics of cervical cancer and the prognosis of different CIN. Results·The positive expression rates of HPV16/18 E6 in normal cervical tissues, CINⅠ , CINⅡ - Ⅲ and cervical cancer group were up-regulated (χ2=19.82, P=0.000). HPV16/18 E6 increased positive expression rates in the low grade and the big size tumors of cervical cancer tissues were detected (P=0.033, P=0.011). There were positive correlations between the overexpression and the pathological grade, tumor size, poor prognosis of cervical cancers respectively (r=0.456, P=0.011; r=0.578, P=0.000; r=0.645, P=0.000).The sensitivity,specificity,and diagnostic accuracy rates of HPV16/18 E6 positive expression to the progression of CINⅠ,CINⅡ-Ⅲand cervical cancer were respectively 100.00%, 62.50%, 43.75%; 96.77%, 91.30%, 92.86%; 96.97%, 83.87%, 66.67%. Conclusion·HPV16/18 E6 overexpression plays an important role in the generation, development and the poor prognosis of cervical cancer. HPV16/18 E6 has a good predictive value for the prognosis and hierarchical management of cervical diseases.
Résumé
Objective:To evaluate the role of human papillomavirus(HPV)16/18E6,cyclin D1,and human telomerase transcriptase(hTERT)in the development of laryngeal carcinoma by detecting the expression of these three genes.Methods:Immunohistochemistry was used to detect the levels of HPV16/18E6,cyclin D1,and hTERT in 42 cases of paraffin-embedded tissues.Results:(1)The positive expression ratios of HPV16/18E6,cyclinD1,and hTERT in the laryngeal carcinoma group were 42.9%(18/42),50%(21/42),and 83.3%(35/42),respectively.These values were significantly higher than that in the control group.(2)The increase in the expression of HPV16/18E6 and cyclinD1 was associated with T stage advance and lymph node metastases.The expression of hTERT increased along with T stage advance,but not with lymph nodes metastases.The changes in the expression of HPV16/18E6,cyclinD1,and hTERT were not associated with gender and age.(3)Positive correlation was observed between HPV16/18E6 and cyclinD1 as well as between HPV16/18E6 and hTERT in laryngeal carcinoma.Conclusion:(1)The expression of HPV16/18E6,cyclin D1,and hTERT may be associated with the development of laryngeal carcinoma.(2)There may be interactions between HPV16/18E6 and cyclinD1,between HPV16/18E6 and hTERT.These interactions may induce the development of laryngeal carcinoma.The information provided by this study may be important for further investigation into the relationship between HPV16/18 and laryngeal carcinoma.