A Review On Pyrazoline Derivatives As Antimicrobial Agent

At present, there is a lot of research about the pyrazoline heterocyclic compound, its ring structure is being changed and new derivatives are being made, many of which have antimicrobial activity over the derivatives. Pyrazoline is the five-member heterocyclic ring which have two N atoms in nearby position and contains two endocyclic double bonds. Noteworthy consideration has been concentrated on pyrazolines and pyrazoline derivative due to their important pharmacological action. Some replaced pyrazolines have been stated near retain particular important pharmacological actions as antimicrobial, antifungal, antineoplastic, antidepressant, insecticidal, anticonvulsant, anti-inflammatory, antibacterial and antitumor properties.

Development of a novel method for UPLC-QTOF-MS analysis of anti-schis-tosomiasis heterocyclic compounds / 中国血吸虫病防治杂志

Objective To develop an ultra-performance liquid chromatography/quadrupole-time of flight mass spectrometry(UPLC-QTOF-MS)method for the determination of an oxadiazole-2-oxide heterocyclic compound F-2015-14.Methods Mouse plasma and liver homogenate specimens were extracted with ethyl acetate and chromatographed on a Waters CORTECS column(C18,1.6μm,2.1 mm×150 mm)by using a mobile phase of 10％acetonitrile-0.1％formic acid with by a volume fractionation by gradient elution.Then,UPLC-QTOF-MS was performed to determine F-2015-14 in mouse plasma and liver homogenate speci-mens.Results The linearity of F-2015-14 in plasma ranged from 12.5 to 250 μg/mL with a correlation coefficient of 0.990 and a detection limit of 8.8 μg/mL.F-2015-14 in liver homogenates ranged from 12.5 to 250 μg/mL.The linearity was good with a cor-relation coefficient of 0.992 and a limit of detection of 5.6 μg/mL.If the concentration of plasma and liver homogenate specimens was 12.5 μg/mL,the accuracy and the matrix effect were 80％to 120％,and the inter-day and intra-day precision was within 20％.If the concentrations of plasma and liver homogenate specimens were 100 μg/mL and 200 μg/mL,the accuracy and the ma-trix effect were 85％to 115％,and the inter-day and intra-day precision was within 15％.Conclusion The UPLC-QTOF-MS es-tablished in this study has a high sensitivity and good reproducibility for the determination of F-2015-14,which provides bases for the development of novel anti-schistosomiasis drugs.