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Introduction: One third of people worldwide are affected with anemia, a condition that is incredibly common. Many times, it is minor and asymptomatic and doesn't need to be managed. Prevalence rises with age and is more prevalent in elderly, pregnant, and females of reproductive age.In people above the age of 85, the prevalence is higher than 20%.In the population of nursing homes, anemia occurs 50% to 60% of the time. A dietary deficit, such as a lack of iron, folate, or vitamin B12, is the main cause of anemia in the elderly, accounting for about one-third of patients. Aim: This study was designed to evaluate the effect of aqueous extract Terminalia cattapa on hematological parameters in phenyl hydrazine induced anemic Wistar rats. Methodology: Five (5) groups (A-E) made up of twenty-five (25) male Wistar rats were assigned at random. Phenyl hydrazine (PHZ) was administered intraperitoneally for seven days at a dose of 10 mg/kg body weight to produce hemolytic anemia. Anemia was assumed to have been induced when the PCV of the PHZ-induced animals was compared to that of the healthy control (non-induced) animals 24 hours following the 7th day of induction. Treatment was administered orally over the course of 14 days using aqueous extracts of Terminalia cattapa leaf at doses of 100 and 200 mg/kg, respectively. Results: The results showed no significant (p<0.05) difference in MCH, MCHC, platelets and MCV when compared with the normal standard and control, but displayed a significant (p<0.05) rise in RBC, WBC, Hb, and PCV. Conclusion: The findings of this study suggest that Terminalia catappa aqueous extract, used in a variety of concentrations, may simultaneously interfere with the osmoregulatory and hemopoietic system of the blood and may be a panacea to anemia.
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Objective: To evaluate new compounds synthesized by integrating quinoline, quinazoline, and acridine rings with the active moiety of (5-nitroheteroaryl) methylene hydrazine. Methods: A new series of compounds (1a, 1b, 2a, 2b, 3a, and 3b) were synthesized and evaluated for cytotoxicity against COS-7 cells using the MTT assay. In vitro anti-plasmodial activity of the compounds was measured against CQ-sensitive (3D7) and CQ-resistant (K1) Plasmodium (P.) falciparum strains. β-hematin assay was performed to assess the inhibitory effects of β-hematin formation for new compounds. Results: The synthetic compounds had anti-plasmodial activity against blood-stage of 3D7 [IC
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Objective: To study the effect of betulin derivatives combination with 5-fluorouracil or hydrazine sulfate on the ROS generation, the SOD and LDH activity using rat blood, as well as the effect of combination drugs on Ehrlich carcinoma in experiments on mice. Methods: We used a chemiluminescence technique to study the ROS generation, and spectrophotometry to determine the MDA level and the SOD and LDH activity. The model of transplanted Ehrlich ascites carcinoma was investigated on mice using a cytological analysis of ascitic fluid cells according to Pappenheim`s method. Results: In vitro experiments on rat blood at the doses of 2, 5 and 10 μg per ml revealed the dose-dependent effect of combination drugs on the antioxidant properties. In plasma, the ROS generation and the MDA level increased by 10-300% in comparison with control at the doses of 5 and 10 μg per ml only. Still, the SOD and LDH activity in general increased by 10-130% in comparison with control under the action of the studied combination drugs. The study on mice showed the effectiveness of a combination of triterpenoids and cytostatics in Ehrlich ascites carcinoma therapy. The state and behavior of the animals improved, the volume of ascites fluid decreased by 40-50% after treatment for 10 d. Conclusion: The combination of betulin derivatives with cytostatics can be used as antitumor drugs in Ehrlich ascites carcinoma therapy that is due to metabolic plasticity, increased ROS generation in enhanced antioxidant enzyme protection.
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OBJECTIVE：To develo p a method for simultaneous determination of chlorogenic acid ，paeoniflorin，hesperidin， ammonium glycyrrhizate ，limonin，nobiletin，tangeretin and lobetyolin in Shenqi jianwei granules. METHODS ：UPLC-Q-Exactive quadrupole electrostatic field orbital hydrazine high resolution mass spectrometry method was adopted. The determination was performed on Hypersil Gold C 18 column with a mobile phase consisted of acetonile- 0.1％ formic acid （gradient elution ）at the flow rate of 0.4 mL/min. The column temperature was set at 35 ℃，the sample size was 1 μL. High resolution full scan experiments was performed over the range of m/z 100-1 000 in heating electro-spray lonization and positive mode to extract the accurate mass of analytes. The contents of 8 components were determined in 3 batches of samples from 2 manufacturers. RESULTS ：The linear range of chlorogenic acid ，paeoniflorin，hesperidin，ammonium glycyrrhizate ，limonin，nobiletin，tangeretin and lobetyolin were 0.12-1.46，3.51-42.15，2.71-32.55，1.76-21.10，0.04-0.46，0.04-0.24，0.02-0.21 and 0.01-0.25 μg/mL，respectively（all r≥0.999 0）. The limits of quantitation were 6.06，0.04，0.03，0.02，7.60，0.05，0.02，6.25 ng/mL，respectively. The limits of detection were 3.03，0.01，0.01，0.01，3.80，0.01，0.01，2.50 ng/mL，respectively. RSDs of precision ，reproducibility and stability （24 h）tests were all lower than 5％（n＝6）. Average recoveries were 93.16％-97.78％（RSD≤5％，n＝6）. The contents of 8 components in 3 batches of samples were 12.62-13.20，265.01-472.26，234.20-278.90，173.10-255.74，2.32-2.83，2.37-3.58，0.79-1.22，0.91-1.78 μg/g，respectively. CONCLUSIONS ：The established method is simple ，convenient，rapid，accurate，highly reproducible and sensitive，which can be used for quality control of Shenqi jianwei granules.
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OBJECTIVE: To establish a method for detecting hydrazine in the air of workplace by high performance liquid chromatography with benzaldehyde derivatization. METHODS: The air samples were collected by glass fiber filter impregnated with sulfuric acid solution and desorbed by sodium dihydrogen phosphate-ethylenediamine tetraacetic acid disodium salt buffer solution. Desorption solution was derived from 1.00% benzaldehyde-acetonitrile solution, then separated by C18 liquid chromatographic column and detected by diode array detector. RESULTS: The linearity range of hydrazine mass was 12.50-150.00 μg, and the correlation coefficient was 0.999 7. The average elution efficiency of hydrazine was 89.28%-95.52%. The within-run and the between-run relative standard deviation were 1.09%-1.86% and 3.63%-4.00% respectively. The samples could be stored at 4 ℃ in a refrigerator for at least 25 days. CONCLUSION: This method has high sensitivity, precision and simple sample preparation. It can be used for detecting hydrazine in the air of workplace.
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A highly sensitive and selective bioluminescent probe for hydrazine (BPH) was designed, synthesized and evaluated for detection of hydrazine in vitro and in vivo. BPH was designed to include a specific recognition group (acetyl) of hydrazine at an appropriate modification site of the optical reporter hydroxyluciferin (D-luciferin), which showed excellent performance both in selectivity and sensitivity to hydrazine. The results showed that the bioluminescent probe BPH developed in this study is an innovative and widely applicable tool for detecting hydrazine in complex natural environment or in animals.
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Objective: To observe the influence of TRPV6 gene silence on SW480 colon cancer cell biological behavior, change in the in-tracellular concentration of calcium, as well as influence of 1,25 (OH)2D3CaCl2and CuCl2on SD rat colonic neoplasm models. Method: SW480 colon cancer cells were infected using lentivirus particles. TRPV6 protein and mRNA expression was detected using immunohistochemical tests, Western blot, and PCR. Moreover, the proliferation, metastasis, and apoptosis of SW480 colon cancer cells were detected through MTT assay and metastasis and apoptosis experiments, and the concentration of Ca2+in SW480 colon cancer cells was measured using high-speed ionic imaging. The SD rat colon cancer model was established based on DMH, and were assigned into experimental group (DMH group, 15) and intervention group (DMH+1,25 (OH)2D3group, DMH+CuCl2group) and control group, 10 in each group. The SD rat colon cancer model is established based on DMH, given 1,25(OH)2D3(37.5 nmol/kg) and CuCl2(375 μmol/kg) separately as intervention. The occurrence of colonic neoplasms and glandular cancers in each group of rats was observed, and Western blot was employed for detection of the TRPV6 protein expression. Results: After the transfection of SW480 colon cancer cells by TRPV6-RNAi, the expression of TRPV6 mRNA and protein decreased, intracellular concentration of Ca2+decreased, proliferation and metastasis rate of SW480 colon cancer cells decreased, and apoptosis rate of these cells increased. The differences between the groups with intervention and the blank control group and negative control group showed statistical significance (P<0.05). The colon cancer occurrence rate in the control group was 0, while that of the DMH+1,25 (OH)2D3 group, DMH group, and DMH+CuCl2were 100%, 84.62%, and 33.33%, respectively. The TRPV6 protein expression was detected in all groups, while DMH+1,25(OH)2D3group was observed to exhibit the highest level of expression, followed by the DMH group, DMH+CuCl2group, and control group. The differences were of statistical significance (P<0.05). Conclusions: The proliferation and metastasis of SW480 colon cancer cells can be prohibited by lowering the concentration of Ca2+in the cells. Thus, the apoptosis of the cells can be induced. 1,25 (OH)2D3can help improve the expression of TRPV6 protein in experimental rat colon tissues and promote the formation of colon neoplasms. CuCl2can help lower the expression of TRPV6 protein in experimental rat colon tissues and prevent the formation of colon neoplasms.
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Palladium hydrogel capped by β-cyclodextrins (Pdβ-CD) was prepared by a facile method with β-cyclodextrins and palladium(II) chloride,which were then modified onto the surface of gold electrode. The morphology and structure of the as-prepared palladium hydrogel were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), while the electrochemistry behaviors of gold electrode modified by Pdβ-CDwere investigated by cyclic voltammetry (CV) and differential pulse voltammetry(DPV). The results indicated the sensor had high electrochemistry response to hydrazine hydrate in the presence of K+,Na+,Mg2+,NH+4,Ni+2,Mn2+,Cl-,NO-3,SO2-4,PO3-4,HCOO-, C6H5O-3. Under the optimized conditions, the oxidized peak current showed linear relationship with the concentration of hydrazine hydrate in the concentration range of 25-950 μmol/L and the limit of detection (LOD) of 1.6 μmol/L(S/N=3).Owing to the facile preparation,high sensitivity and selectivity,the sensor has potential applications in determination of hydrazine hydrate in real water samples
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A fluorescence enhancement probe (ZY8) for the detection of N2H4was designed and synthesized by employing 3-hydroxyflavone as a fluorophore,and its spectral properties had been investigated. The results showed that ZY8 had relatively good selectivity and specificity to N2H4in Tris-HCl-ethanol solution (9:1, V/V, pH 7.40). The fluorescent intensity of ZY8 exhibited good linear relationship with concentration of N2H4in the concentration range of 1.6×10-7mol/L-6.2×10-5mol/L,and its detection limit was estimated to be 1. 6×10-7mol/L. ZY8 itself had weak fluorescence, upon addition of N2H4, an approximate 9-fold fluorescence enhancement was observed, and the color of the solution changed from light grayish green to bright grass-green at UV light of 365 nm. So ZY8 might be used to the visual recognition of N2H4. ZY8 could detect N2H4in near-physiological pH range, and it had fast response and strong anti-interference ability. Moreover,ZY8 could be loaded as test paper for naked-eye detection of N2H4at mmol/L level in water solution,and it was also applied in the determination of N2H4in various water samples by the standard addition recovery experiments, with the recovery ratio ranged from 96.0% to 104.2% %, and RSD of all< 4%. The results of this study demonstrated that ZY8 had potential application to the detection of N2H4in the monitoring of environmental pollution.
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Objective To establish a zebrafish model of thrombosis induced by three kinds of inducers and observe the anti?thrombotic effect of a Chinese traditional medicine, Guanxinning tablet ( GXN) . Methods The zebrafish models of thrombosis was induced by using 1?5μmol/L phenyl hydrazine, 80μmol/L arachidonic acid and 5 mg/L ponatinib, re?spectively, and were treated with various concentration of GXN, clopidogrel or asprin. The thrombus in the tail vein was observed under microscope, Erythrocytes in the zebrafish heart were stained with o?dianisidine and the erythrocyte staining intensity was assessed with a NIS?Elements DTM image analyzer, and the anti?thrombolic effect of GXN was calculated. Results Venous thrombus was significantly increased and the staining intensities of erythrocytes in the heart were signifi?cantly decreased after induction by phenyl hydrazine, arachidonic acid or Ponatinib ( P <0?001 ) , respectively. At the same time, GXN showed an incresing anti?thrombolic effect in the zebrafish models (P<0?001) in a dose?effect manner, with a IC50 of GXN of 44?32 mg/L,138?5 mg/L and 459?5 mg/L, respectively. Conclusions The zebrafish models of thrombosis are successfully established by phenyl hydrazine, arachidonic acid or Ponatinib, respectively, by different for?mation mechanisms. GXN has been shown to have an anti?thrombosis effect, probably, by multiple target effects.
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Para o processo da eletrooxidação da hidrazina sobre a camada do composto carbonílico derivado de ferroceno, foi sugerido o mecanismo do desempenhoeletroanalítico. Para ele, também foi desenvolvido e analisado (por meio da teoria de estabilidade lineal e análise de bifurcações) o modelo matemático, capaz de descrever os processos no sistema. Os resultados da modelagem foram comparados com os experimentais, bem como com os teóricos, observados para sistemas análogos.
For the process of hydrazine electrooxidation over the layer of ferrocene-carbonylic derivative, the electroanalytic functionmechanism has been suggested. Also there has been developed and analyzed (by means of linear stability theory and bifurcation analysis) the mathematical model, capable to describe the processes in the system. The results of the modeling have been compared with experimental and theoretical for analogous systems.
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Objective: To isolate, purify, and analyze the anti-oxidants from the leaves of Lantana trifolia. Methods: The anti-oxidative activities of the crude extracts from liquid-liquid extraction of L. trifolia leaves were assayed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method to assess their radical scavenging and reducing abilities. The total flavonoids and phenol contents in the ethyl acetate fraction were determined by colorimetric and Folin-Ciocalteu methods, respectively. Chemical constituents were isolated from the ethyl acetate fraction and repeatedly purified using silica gel, Sephadex LH-20 column chromatography, and HPLC, respectively. The chemical structures isolated were identified by spectral analysis and chemical evidence. Results: Ethyl acetate partition from liquid-liquid extraction exhibited the highest anti-oxidative activity with an IC50 value of 4.94 μg/mL, close to that of the standard (vitamin C, VC, 4.23 μg/mL). The extract was proved to contain total flavonoids and phenol contents with values of (39.0 ± 1.6) and (29.27 ± 1.46) mg/g, respectively. Six compounds were isolated and identified as kaempferol-3,7-dimethyl ether (1), verbascoside (2), apigenin (3), umuhengerin (4), ladanetin (5), and scutellarein-7-O-β-D-apiofuranoside (6). Conclusion: The ethyl acetate extract from the leaves of L. trifolia possesses the potent anti-oxidative and free radical scavenging activities which are directly proportional to the concentration of phenolic contents. The anti-oxidative activity of the extract from the leaves of L. trifolia is due to its proton donating ability that converts free radicals to more stable products and terminates chain reactions. Compound 1 is isolated from the plants of Lantana Linn. for the first time. The mechanisms may be related to the therapeutic benefits of the certain traditional claims of wild L. trifolia. © 2014 Tianjin Press of Chinese Herbal Medicines.
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Extraction and spectrophotometric determination of Uranium (VI) was carried out using Hydrazine Carboxamide-2-[(2-Hydroxy-1-Naphthalenyl) Methylene] as an analytical reagent. A reddish brown coloured complex is formed at pH 9.2 between U(VI):HCHNM which is extracted in n-butanol. An intense peak for the complex was observed at 385nm, well separated from that of ligand. Beer’s law is obeyed over the range of 2 to20 ppm. Composition of M:L in the complex was ascertained by Job’s method, mole ratio method and slope ratio method and found to be 1:2. Extraction and spectrophotometric determination of U(VI) was carried out using Hydrazine Carboxamide-2-[(2-Hydroxy-1- Naphthalenyl) Methylene] as an analytical reagent. A reddish brown coloured complex is formed at pH 9.2 between U(VI):HCHNM which is extracted in n-butanol. An intence peak for the complex was observed at 385nm, well separated from that of ligand. Beer’s law is obeyed over the range of 2 to 20 ppm. Composition of M:L in the complex was ascertained by Job’s method, mole ratio method and slope ratio method and found to be 1:2. Diverse ion effect was studied for various ions and the proposed method was found to be highly selective for the trace concentration of U(VI). Molar absorptivity and Sandell sensitivity values calculated are 0.1566 x104L mol-1cm-1 and 0.0585g/cm2 respectively. Proposed method has been successfully applied to analyse synthetic samples and the results were found to be in good agreement with standard gravimetric methods.
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New complexes of Co(II), Ni(II), Cu(II), Cd (II), Pt(II),and Pd(II) with 4-(trifluoro-4-ylidene) hydrazine carbothioamide have been synthesized. All the new compounds were characterized by elemental analysis, molar conductance measurements, magnetic susceptibility measurements, LC-MS. H1NMR, FTIR and electronic spectral studies. Based on the molar conductance measurements in DMF the complexes may be formulated as [Ni(L)2Cl2] and [M(L)2]X2 (where M = Co (II), Cu (II), Cd (II), Pt (II) and Pd(II) and X = Cl - , CH3COO - due to their non electrolytic nature respectively. The antimicrobial activities of the ligand and their complexes have been studied by screening the compounds against the bacteria E. coli and S. aureus and also the fungi Asperillius niger and candida albicans and results have been compared with standard drug streptomycin and fluconazole. The data indicate that the metal complexes have higher antimicrobial activity than the free ligand and the standard drug. The DNA cleavage experiments, performed using gel electrophoresis with the corresponding metal complexes in the presence of H2O2 showed that all the complexes afford a pronounced discernible DNA cleavage evidenced by the disappearance of form I (supercoiled) of DNA and the production of formII (Linear).
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Aims: To find out the effect of crude extract of Oscillatoria willei, a marine cyanobacterium on hydrazine induced toxicity. Methodology and Results: In this study, the experimental mice were injected intramuscularly with 5 mg of hydrazine/kg body weight continuously for 20 days. Crude extract of Oscillatoria willei was given to the animals induced with hydrazine toxicity. The animals were subjected to various biochemical and immunological parameters after exposing to hydrazine and followed up treatment. The results revealed that intra-peritoneal administration of O. willei reduced interleukin 2 (IL-2), reducing sugar, thiobarbituric acid reactive substances (TBARS), liver enzymes, bilirubin, creatinine and uric acid level. Conclusion, significance and impact of study: O. willei treatment was found to reduce the ill effects induced by hydrazine.
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The effect of hydrazine on blue membrane was investigated by the UV/VIS absorption spectrum technique and the flash photolysis technique, the results show that: hydrazine can convert blue membrane to purple membrane and the photocycle returns, but the rate of decay of photcycle intermediate(M412) quickens, this phenomenon is not seen when metal cations are added to blue omembrane solution(the rate of decay of photcycle intermediate slowers). At the same time , the effect of pH and temperature on the interaction between hydrazine and blue membrane was investigated. When hydrazine was added to blue membrane solution, the sensitivity of the reaction is pH and temperature dependent. Over the pH range(2～4.8), the sensitivity of the reaction lowers with the increase of the acidity. Over the temperature range(20～40℃), the sensitivity of the reaction lowers with the increase of the temperature.
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Objective To develop a nonaqueous electrolyte electrochemical detector in order to solve the problems of shorter average life span and operating temperature range in aqueous electrolyte electrochemical detector for unsymmetrical dimethyl hydrazine(UDMH).Methods Nonaqueous electrolyte was utilized in this kind of electrochemical detector comprised of n-mehyl-2-pyrrolidone with conductive compatible salts,such as tetrabutyl ammonium tetrafluoroborate,which had much lower evaporating pressure and freezing point than aqueous electrolyte ones.Three-electrode potential electrochemical system was also used,composing a sensing electrode,a counter electrode and a reference electrode.The three electrodes were all teflon-bonded diffusion electrodes which were prepared by spraying the noble metal catalyst dispersion onto the Teflon film.Results The experiment result showed that the average life span could be over two years compared with the aqueous electrolyte electrochemical detector of one year life span.Operating temperature range was expanded to-20 ℃～+40 ℃.However,the response time was nearly five minutes.Conclusion The detector can be used for leakage checking in the places where a large quantities of UDMH are used or stored.
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Objective To make a accurate, simple method for determination which is suitable for application in basic level units to test nitrate nitrogen in drinking water. Methods Based on the principle that catalyzed by the Cu2+, NO3- is reduced by hydrazine sulfate into NO2-, then it reacts on sulfanilamide in hydrochloric acid to produce a diazo compound, the latter reacts on NEDD to produce a rose dye. Results The maximum wave-length of the was 555 nm, the molar absorption coefficient was 6.5?106L/(mol?cm), RSD were 0.55%-2.07%, the rates of recovery were 91.3%-109.6%. Beer's law was obeyed in the range of 40-320 ?g/L(1-8 ?g /25 ml). Conclusion This method is simple, rapid, accurate and suitable for application in basic level units to determine nitrate nitrogen in drinking water.