Résumé
Objective: To study the chemical constituents in extract of Sanguisorba officinalis. Methods: Compounds were isolated from 10% ethanol extracts by Macroporous resin (D101, HP-20), dialysis bag, reversed phase silica gel (RP-8, RP-18), Toyopearl HW-40 column chromatography and their structures were elucidated by NMR and MS data. Results: Twenty-five compounds were isolated and their structures were elucidated as 4-(4’-hydroxyphenyl)-2-butanone-4’-O-β-D-glucopyranoside (1), phenethanol-β- vicianoside (2), junipetrioloside A (3), citroside A (4), corchoionoside C (5), adenosine (6), tryptophan (7), tachinoside (8), d-mandelic acid-β-D-glucopyranoside (9), (+) (7S,8S)-guaiacylglycerol 8-O-β-D-glucopyranoside (10), biophenol (11), 3,5-dihydroxyphenethyl alcohol 3-O-β-glucopyranoside (12), syringin (13), (2E,5E)-3,7-dimethyl-2,5-octadiene-1,7-diol (14), (±)-3-hydroxy-3,7-dimethyloct- 6-enoic acid (15), (2Z)-2,6-dimethyl-2,7-octadiene-1,6-diol (16), phlorizin (17), (+)-cyclo-olivil 6-O-β-D-glucopyranoside (18), 5’-methoxy-8’-hydroxyl-(+)-isolariciresinol-4’-O-β-D-gluco-pyranoside (19), phenethyl 6-O-α-L-arabinofuranosyl-β-D-glucoside (20), gaultherin (21), benzyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (22), (2E)-7-hydroxy-3,7-dimethyl-2-octenyl 6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (23), 3,3’,4’-tri-O-methylellagic acid (24), and methyl-4-(β-D-glucopyranosyloxy)- 3-hydroxy-5-methoxybenzoate (25). Conclusion: Compounds 1-22 were isolated from this plant for the first time.
Résumé
The hydrosoluble active constituent of Salvia miltiorrhiza Bge has a wide range of physiological activities and pharmacological effects.Endothelial progenitor cells(EPCs) comprise a cell population that has the capacity to circulate, proliferate and differentiate into mature endothelial cells but that has not yet acquired characteristic mature endothelial markers nor formed precursor cells of blood vessels.It was found that the hydrosoluble active constituent of Salvia miltiorrhiza Bge could protect endothelial functions through modulation of EPCs functions, thus exerting therapeutic effects in endothelium injured related diseases.This paper reviewed the effects of the hydrosoluble active constituent of Salvia miltiorrhiza Bge on EPCs functions.We hope that the paper could provide a reference for further utilization of hydrosoluble active constituent of Salvia miltiorrhiza Bge.
Résumé
Objective To study the distribution and significance of free organophosphorus poisons (FOP)with different degrees of hudrosolubility in the blood and adipose tissues of poisoned rabbits.Methods Seventy two male livid blue rabbits with 2-2.5 kg body weight were divided into 3 groups as per the rabbits intoxicated with different kinds of organophosphorous given subcutaneously,namely monocrotophos(11.12 mg/kg)group,trichloffon(556.0 mg/kg)group and methyl parathion(37.05 mg/kg)group(n =24 in each).Each group was further divided into 4 subgroups(n =6,in each).Blood samples and adipose tissues were collected 1 h,6 h,24 h and 96 h after administration of organophosphorous.Blood cells and plasma were separated.Well-formed adipose tissue homogenates were made.Acetylcholinesterase(AChE)activities were assayed with dithiobisnitrobenzoic acid(DTNB)enzyme kinetic method.The levels of FOPs in blood plasma,blood cells and adipose tissues were determined with enzyme inhibition method.Results There were signiflc.ant differences in FOP concentrations of plasma,blood cells and adipose tissues among moncrotophos group,trichlorfon group and methyl pararthion group at the intervals of 1,6 and 24 hours after organophosphorous administered(all P ＜ 0.05).There were no significant differences in FOP concentrations of moncrotophos among blood plasma,blood cells and adipose tissues at intervals of 1,6,24 and 96 hours.FOP concentrations of trichlorfon in adipose tissues were significantly higher than those in blood cells and plasma at intervals of 1,6,24 hours after organophosphorous administered(P ＜ 0.05).There were significant differences in FOP concentrations of pararthion in blood plasma compared with blood cells and adipose tissues at intervals of 1 and 6 hours after organophosphorous administered(P ＜ 0.05)and no difference was found over 24 hours after administration of organophosphorous(P ＞ 0.05).The significant differences in the rates of FOP clearance from blood and adipose tissues were also found between different organophosphates(P ＜ 0.05,moncrotophos ＞ trichlorfon ＞methyl pararthion).Conclusions With the decrease in hydrosolubility of organophosphates,the increase in adipose tissue-specific retention was found and the time required for clearance from blood and adipose tissue was prolonged.Adipose tissue was the major storage site for organophosphates with low hydrosolubility.