Résumé
Objective: An effort currently made to appraise the preliminary phytochemical, pharmacognostic criteria, antioxidant, GCMS and antihyperglycemic investigations of the Thunbergia coccinea leaves. Thunbergia coccinea (T. coccinea) is an ornamental plant considerably practiced by the tribes of forest areas of Assam (INDIA) as an analgesic, antipyretic, anti-inflammatory, antidote, hepatoprotective, antidiabetic and detoxificant substance. Methods: A comprehensive literature survey was conducted to recognize the ethnomedicinal value of T. coccinea, which is currently grown practically in all provinces. The physicochemical constants like moisture content, ash values especially total ash, insoluble acid ash, water-soluble ash and foreign organic matter were determined for the assessment of the drug. Pharmacognostic parameters like fluorescence examination and microscopic characters of the leaf were studied that would serve to verify for contamination. The extract secured by maceration was subjected to the phytochemical inquiry to determine the existence of substances and their antioxidant activity. The antihyperglycemic characteristic of alcoholic extract of the leaf was examined with the inhibition of α-amylase and α-glucosidase enzymes. Gas Chromatography-Mass Spectrometry (GCMS) studies of alcoholic extract of the plant leaf have undertaken to get an insight into the therapeutic properties of the molecules present based on online PASS prediction. Results: Various physicochemical, microscopic parameters studied gave a clear distinguishing and identifying features of T. coccinea leaf. Phytochemical screening gave an insight into the secondary metabolites existing in the plant leaf through picturizing its therapeutic properties against various ailments. Both extracts of T. coccinea leaf showed enhanced antioxidant activities. Nevertheless, the alcoholic leaf extract has shown significant antioxidant activity with an IC50 of 171.38±2.51 μg/ml and AQTC an IC50 value of 206.29±4.5 μg/ml respectively by DPPH method. Further, ACTC showed a better-reducing potential with an IC50 value of 105.74±0.61 μg/ml in comparison with AQTC IC50 value of 203.702±0.97 μg/ml by FRP method. The inhibition potentiality of α-amylase and α-glucosidase was found to be 71.66 % and 83.74 %, respectively at 500 µg/ml that rationally an adequate remedy in the treatment of type-2 diabetes. GCMS studies of the alcoholic extract unveiled the presence of different molecules like Glycerol, tris (trimethylsilyl) ether, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, Undecanoic acid, Ethyl ester, Phytol in comparison with NIST library, thereby giving its predicted therapeutic properties like sugar phosphatase inhibitor, antifungal, phobic disorders treatment, antiviral and so on. Conclusion: The selected plant had many proven therapeutic traits and, possibly, successively united on to the sort of potential therapeutic plants. Besides, isolation and discoveries will lead to the detection of certain novel compounds, which will be of potential medicinal value.
Résumé
OBJECTIVE: To establish a method to determine three main flavones in the extract of rhizomes and frond bases of Matteuccia struthiopteris and evaluate the antioxidative activity of the extract. METHODS: The contents of the flavones in the extract were determined by HPLC. Kromasil C18 column was used. Methanol-0.1% orthophosphoric acid solution (70:30) was used as the mobile phase at a flow rate of 1.00 mL· min-1. The detection wavelength was set at 295 nm and the column temperature was 40°C. The antioxidative activity of the extract was detected by DPPH free radical scavenging assay. IC50 (the antioxidant concentration for scavenging half of DPPH radical) was used as the index to evaluate scavenging capacity. RESULTS: The calibration curves of demethoxymatteucinol, matteucinol, and matteuorien showed good linearity over the ranges of 0.058 - 0.58, 0.045 - 0.45, and 0.003 - 0.028 mg· mL-1 (r = 0.9998, 0.9998, 0.9983), respectively. The average recoveries were 96.93%, 97.15%, and 96.41%, respectively. The IC50 value of the extract of rhizomes and frond bases of Matteuccia struthiopteris was 0.55 mg· mL-1. CONCLUSION: The quantitative determination method with good accuracy, repeatability and stability is suitable for the quality control of the extract of rhizomes and frond bases of Matteuccia struthiopteris. The extract has potential antioxidative action.