Résumé
OBJECTIVE: To improve the quality evaluation method of Fritillariae Cirrhosae Bulbus by the means of objective quantization of appearance character. METHODS: The height and diameter were measured by a vernier caliper. The color of powder was determined by a colorimeter based on the human eye observation. The content of total alkaloids was measured by the ultraviolet spectroscopy. Some correlation analysis between the content of total alkaloids and height, diameter, and △E value were evaluated by a SPSS software, respectively. RESULTS: There were a very significant negative correlation (P0.01) between height and the total alkaloids, a negative correlation between diameter and the total alkaloids, and no significant correlation between △E value and the total alkaloids. CONCLUSION: Because the content of total alkaloids could reflect the quality of Fritillariae Cirrhosae Bulbus objectively, these RESULTS: of this study provide a scientific basis for the traditional ideas of “the smaller the best” and “the broken Fritillariae Cirrhosae Bulbus could not be used as medicine” and affirm the traditional processing technology that “should not be washed by water”. Songbei was the best Fritillariae Cirrhosae Bulbus of them all.
Résumé
Object To develop a method for the quantitative analysis of peiminine, imperialine and imperialine 3? D glucoside in Fritillaria L by capillary electrophoresis Methods 9 species of bulbus Fritillaria L. were analysed with internal standard by the mode of capillary zone electrophoresis Results The 3 alkaloids were separated completely with the method which showed good recovery and reproducibility Conclusion The method was proved to be quick, simple, and efficient, and provided a reliable basis for the quality control and evaluation of this plant medicine
Résumé
Objective: To compare the extraction efficiency of imperialine extracted with different solvents. Methods: The content of imperialine in the extracts was determined by thin layer chromatography scanning. Results: Among the methods suitable for alkaloid extraction, the solvent ether chloroform 95% ethanol (25∶8∶2.5) is the best for imperialine extraction. Conclusion: The imperialine extraction efficiency is greatly affected by the extraction solvent.