TLR4 mediated the nuclear translocation of transcription factor NF-κB and IRF-3 in HTNV infected vascular endothelial cells / 中华微生物学和免疫学杂志

Objective To observe the nuclear translocation of transcription factor NF-κB and IRF-3 in TLR4 silenced EVC304 cells infected by HTNV and to provide new information for anti-HTNV innate immunity and its signal transduction. Methods TLR4~- cells and TLR4~+ cells were infected by HTNV 76-118, respectively. The cells stimulated by LPS were selected as positive control groups, and the cells without stimulation were selected as negative control groups. After 6 hours, indirect immunofluorescence assay(IFA) was used to detect the nuclear translocation of NF-κB and IRF-3. Results The transcription factor NF-κB and IRF-3 transfered into nuclear 6 hours after stimulated by HTNV 76-118. Conclusion TLR4 may mediate the nuclear translocation of transcription factor NF-κB and IRF-3 in HTNV infected human umbilical vein endothelial cells.

Characterization of promoter of human interferon regulatory factor 3 / 中华微生物学和免疫学杂志

ays revealed that the-(167-111)bp region Was the minimal promoter of the human IRF3 gene.These results suggested that transcriptional factors such ag E2F might be involved in the transcriptional regulation of IRF3 gene.