RÉSUMÉ
@#AIM: To explore the levels of interleukin-8, interleukin-8 receptor 1, 2(CXCR1, CXCR2)in aqueous humor of polypoid choroidal vasculopathy(PCV)and their clinical significance.<p>METHODS: Totally 67 patients with PCV in our hospital were selected as PCV group, and another 50 patients with cataract were selected as control group, fluorescence quantitative PCR(QPCR)was used to detect the expression of IL-8, CXCR1 and CXCR2 mRNAs in aqueous humor of two groups, the best corrected visual acuity(BCVA)of all subjects were measured by the international standard visual acuity chart, optical coherence tomography(OCT)was used to detect foveal retinal thickness(CMT), the receiver operating characteristic curve(ROC)was created to analyze the predictive values of IL-8, CXCR1 and CXCR2 in the occurrence of PCV. Multivariate Logistic regression analysis was used to assess the risk factors for PCV.<p>RESULTS: Compared with the control group, the expression of IL-8, CXCR1 and CXCR2 mRNA increased in group PCV. BCVA in PCV group decreased and CMT increased(<i>P</i><0.05). IL-8, CXCR1 and CXCR2 were significantly positively correlated with BCVA(<i>r</i>=0.438, 0.346, 0.385, all <i>P</i><0.05), IL-8, CXCR1 and CXCR2 were positively correlated with CMT(<i>r</i>=0.378, 0.606, 0.357, all <i>P</i><0.05). Logistic multivariate regression analysis showed that IL-8, CXCR1, CXCR2, BCVA and CMT were the risk factors of PCV. The AUC of IL-8, CXCR1 and CXCR2 for PCV diagnosis was 0.882, 0.860 and 0.812.<p>CONCLUSION: The expression of IL-8, CXCR1 and CXCR2 in PCV aqueous humor were all increased, which was not only related to BCVA and CMT in patients, but also related to the occurrence of PCV, and it may become a potential predictor of PCV.
RÉSUMÉ
Interleukin 8 (CXCL8) is an autocrine chemokine specific for the chemoattraction and activation of granulocytes, NKT cells and T lymphocytes. Patients with tuberculosis and latent Mycobacterium tuberculosis infection were assessed for the spontaneous expression of CXCR1 (CD128) and CXCR2 on lymphocytes and monocytes. Compared with ex vivo profiles, increased spontaneous CXCR2 expression and normal CXCR1 expression were found on lymphocytes in two out of 59 individuals. Monocytes showed normal ex vivo profiles for both receptors. After stimulation with purified protein derivative, the in vitro levels of CXCL8 were below the median levels of all patients with prior tuberculosis. Spontaneous CXCR2 modulation did not cause notable variation in the in vitro levels of CXCL8.
Interleucina 8 (CXCL8) é quimiocina autócrina específica para atração e ativação de granulócitos, assim como NKT e linfócitos T. Pacientes com infecção por Mycobacterium tuberculosis e latentes foram recrutados para comparar expressão espontânea dos receptores CXCR1 (CD128) e CXCR2 nos mononucleares. Comparado com perfis ex vivo dos linfócitos, observou-se aumento em CXCR2; porém, expressão normal de CXCR1 em dois dos 59 indivíduos. Monócitos mostraram perfis ex vivo normais; após estimulação específica in vitro das citocinas estudadas com extrato bruto total, não se encontrou correspondência na anomalia observada ex vivo. Modulação espontânea de CXCR2 não causou grande variação in vitro nos níveis de CXCL8.