Résumé
Objective: To investigate the protective effect of Jinlida granules on hippocampus of diabetic rats. Methods: Diabetic models were induced by high-fat diet and intraperitoneal injection of streptozotocin(STZ) in SD rats. The study was divided into diabetic model group, Jinlida granule groups(0. 75, 1. 5,and 3. 0 g/kg), α-lipoic acid group and insulin group. Healthy rats served as normal controls. Rats were tested in Morris water maze after 8 weeks of treatment, and then the hippocampus tissues were taken from each group and were infjected to TUNEL assay and transmission electron microscopy (TEM) observation. The activities of superoxide dismutase (SOD), glutathione (GSH), myeloperoxidase (MPO), and the contents of malondialdehyde (MDA) were all examined. Results: The diabetic rats developed studying and memory disorders 8 weeks after establishment. Except for those in the low-dose Jinlida group, rats in other treatment groups had improved studying and memory functions to different extents. Compared with normal control group, rats in the model group had more apoptotic neurons in the hippocampal CA1 area, prominent ultrastructure damage, significantly decreased SOD and GSH activities (P<0. 05), and significantly increased MPO activity and MDA level (P<0. 05). Compared with the model group, rats in all the treatment groups had decreased apoptosis and less ultrastructure damage;and rats in high-dose Jinlida group, α-lipoic acid group and insulin group had significantly increased SOD and GSH activities and significantly decreased MPO activity in the hippocampal CA1 area (P<0. 05); and the high-dose Jinlida granule group had a similar effect to α-lipoic acid group. Conclusion: Jinlida granule can protect the hippocampus of diabetic rats.
Résumé
Objective to investigate the protective effect of Jinlida granules on islet β cells in diabetes mellitus rats. Methods Diabetic models were induced by high-fat diet and intraperitoneal injection of streptozotocin (STZ) in SD rats. The study was divided into model group, Jinlida granule group (0.75, 1.5, 3.0g/kg) α-lipoic acid group, insulin group, and metformin group. Each group was given corresponding drugs, with insulin given by subcutaneous injection and other drugs given by intragastric administration. The body weight, fasting blood glucose (FBG), fasting insulin (FINS), glycosylated hemoglobin (HbAlc), the activities of super oxide dismutase (SOD) and glutathione (GSH), and the contents of malondialdehyde(MDA), IL-10 and TNF-« were examined 2 months later. The pancreatic tissues were immunostained for insulin. Results Compared with the model group, the levels of FBG and HbAlc were decreased in other groups (P<0. 05, P< 0. 01). The activities of SOD and GSH were significantly increased (P<0. 01) and the levels of MDA, IL-1β and TNF-α were significantly decreased (P<0. 01) In high-dose (3. 0 g/kg) Jinlida granule group, with increased islet area as shown by pathological staining. Conclusion Jinlida granule has protective effect against STZ-induced islet β cell injury in diabetic rats.
Résumé
AIM:To observe the influence of the different bore diameter ceramic membranes on the processing parameters of Jinlida Granules(Radix et Rhizoma Salviae miltiorrhizae,Radix Sophorae flavescentis,Rhizoma Polygonati,etc.) and optimize the process of membrane filtration. METHODS: Three different membranes were tested to observe the changes in membrane flux and the content of effective components by Jinlida Granules extract. RESULTS: The 50 nm membrane had the great flux,the transfer rates of matrine and salvianolic acid were the highest;The optimum conditions: the operation differential pressure was 0.08～0.12MPa;the operation temperature was 50～60℃;the membrane surface flow rate was 3.0 m/s.When the volume of filtration solution was condensed to one tenth,adding the same volume aeionized water,and the effective components transfer rate reached 80%.Using the strong acid and strong alkali to wash it in turn,the flux can revivify above 90%. CONCLUSION: Applying the ceramic membrane to ultrafiltrate Jinlida Granule is good and useful to herb purification.