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Kaurenoic acid oxidase involved in biosynthesis pathway of gibberellin. According to the transcriptome database, the specific primers were designed and used in cloning the full-length cDNA of TwKAO, the bioinformatic analysis of the sequence was performed. The qRT-PCR were used to detect the expression level of TwKAO after MeJA treatment.The full-length cDNA of the TwKAO was 1 874 bp encoding a polypeptide of 487 amino acids.The calculate molecular weight was about 56.02 kDa,and the theoretical isoelectric point (pI) was 8.89. The relative expression level of TwKAO was deduced by MeJA and reached the highest at 12 h after the treatment.Plant tissue expression analysis indicated that, TwKAO expressed the highest in leaves,while lowest in roots.For the first time, we cloned and analyzed the expression characteristics of TwKAO, which laid a foundation for deep analysis of growing development and terpenoid secondary metabolites in T. wilfordii.
Résumé
Objective] To summarize clinical experience of Chen Yi’s Yi Zhi Zhu Kao Gao Fang. [Methods] By means of analyzing the principle of prescription,syndrome differentiation and characteristics,combined with typical cases, experiences of Chen Yi’s Yi Zhi Zhu Kao Gao Fang were summarized. [Results] Chen Yi emphasizes the key of Yi Zhi Zhu Kao Gao Fang on balancing the five internal organs, analyzing the heart, liver, spleen and kidney’s function. He uses the way of regulating liver-qi for easy mood, nourishing heart and appeasing spirit to sequence sleeping, strengthening spleen and stomach to regulate middle energizer, replenishing qi to consolidate the superficial resistance, tonifying kidney to consolidate the constitution, respectively. Taking it in low dosage in long time can get the purpose of Yi Zhi Zhu Kao. [Conclusion] Chen Yi ’s Yi Zhi Zhu Kao Gao Fang has clear theory, rigorous formula, curative effect and worth passing on promotion.
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Object To study the anthraquinone constituents in rhizoma and radix of Rheum sublanceolatum C Y Cheng et T C Kao Methods The constituents were isolated through column chromatography, and their structures elucidated through physicochemical and spectral analysis Results 6 anthraquinones were isolated and identified as chrysophanol (Ⅰ); physcion (Ⅱ); emodin (Ⅲ); emodin 8 O ? D glucopyranoside (Ⅳ); aloe emodin 8 O ? D glucopyranoside (Ⅴ) and citreorosein (Ⅵ) Conclusion All these compounds were isolated from R sublanceolatum for the first time