Perspectivas en investigación: células HEp-2 infectadas con EB's de Chlamydia trachomatis serovar 2 (VR-902B) / Chlamydia trachomatis: Research Perspectives. HEp-2 Cells Infected with EB's from Chlamydia trachomatis serovar 2 (VR-902B): Research Perspectives

Resumen Chlamydia trachomatis (C. trachomatis) es una bacteria Gram negativa inmóvil, caracterizada por ser un microorganismo intracelular obligado y por poseer un ciclo reproductivo en el que puede distinguirse una forma infecciosa extracelular metabólicamente inerte (cuerpo elemental - EB's), y una forma no infecciosa intracelular y activa (cuerpo reticulado - RB's). C trachomatis se caracteriza por causar infección en humanos, está relacionada con enfermedades de transmisión sexual e infecciones oculares; por lo que puede conllevar a secuelas de interés, si no se da un tratamiento oportuno. El objetivo de este estudio fue optimizar el modelo de infección de C. trachomatis en células HEp-2 con cuerpos elementales (EB's) de C. trachomatis serovar L2. Inicialmente, se establecieron las condiciones para el crecimiento adecuado de las células HEp-2 en tiempo y con una confluencia del 90%, para continuar con la optimización de un protocolo de infección. La infección fue confirmada a partir de la coloración con Giemsa permitiendo evaluar características morfológicas tanto de las células HEp-2 sin infectar e infectadas, y así mismo, de los cuerpos elementales de C. trachomatis. Finalmente, se corroboró la infección con la técnica de inmunofluorescencia directa que detecta la proteína de membrana MOMP de C. trachomatis. Tras los ensayos realizados se evidenció la presencia de cuerpos elementales próximos y dentro del citoplasma celular, así como células vacuoladas y daño celular causado por la infección.

Abstract Chlamydia trachomatis (C. Trachomatis) is a Gram negative unmoving bacterium, characterized by being an obligate intracellular microorganism and having a reproductive cycle in which a metabolically inactive extracellular infectious form (elementary body - EB's) can be distinguished from an intracellular active and non-infectious form (reticulated body - RB's). C trachomatis is characterized by causing infection in humans, is related to sexually transmitted diseases and eye infections, so it can lead to sequelae of interest if timely treatment is not given. The objective of this study was to optimize the infection model of C. trachomatis in HEp-2 cells with elementary bodies (EB's) of C. trachomatis serovar L2. Initially, the conditions for the adequate growth of HEp-2 cells were established in time and with a confluence of 90%, to continue with the optimization of an infection protocol. The infection was confirmed from the staining with Giemsa allowing to evaluate morphological characteristics of both uninfected and infected HEp-2 cells and also of the elementary bodies of C. trachomatis. Finally, the infection was corroborated with the direct immunofluorescence technique, that detects the C. trachomatis MOMP membrane protein. After the tests were performed, the presence of elementary bodies nearby and within the cellular cytoplasm was evidenced, as well as vacuolated cells and cellular damage caused by the infection.

Clinicoepidemiological profile of sexually transmitted disease (STD) patients presenting in a tertiary care center

Background: Sexually transmitted infections (STIs) constitute a major health problem in the developing countries and prevalence of STIs varies from region to region in our country. Aim: To study the pattern and prevalence of sexually transmitted infections and HIV seropositivity among patients presenting in a tertiary care center. Materials and methods: A retrospective data was collected in the department of Dermatology at Shri Guru Ram Rai Institute of Medical and Health Sciences (SGRRIHMS), Shri Mahant Indiresh Hospital (SMIH), a tertiary care center in Dehradun, Uttarakhand, India, from January 2011 to December 2018. A total of 525 patients were analyzed in this study. Results: A total of 525 patients were enrolled in our study. STIs were more common in men, with male (365): female (160) ratio of 2.2:1. Maximum numbers of patients were in the age group of 25 to 34 years. Unskilled workers (22.28%) and housewives (20.00%) constituted major proportion. Majority of the cases presented with warts (38.09%) followed by molluscum contagiosum (20.95%) and herpes genitalis (19.61%). But herpes genitalis (27.39%) was found to be the most common STD seen among males and warts (68.75%) among the females. HIV seropositivity was seen among 20 (3.80%) patients and was most commonly associated with herpes genitalis infection (60.00%). Gunjan Gupta, Jitendra Singh Bist, Rahul Sudan, Kewal Krishan, Neeti Kumari. Clinicoepidemiological profile of sexually transmitted disease (STD) patients presenting in a tertiary care center. IAIM, 2019; 6(2): 7-12. Page 8 Conclusion: Increased prevalence of viral STDs like herpes genitalis, genital warts and mollascum contagiosum was seen in our study. Widespread use of antibacterial lead to falling trend of bacterial STDs.

Importancia clínica de las Chlamydias / Clinical significance of Chlamydias

Las infecciones por Chlamydia han alcanzado alta incidencia en el mundo. Constituyen importantes patógenos para los animales y el hombre. En la especie humana pueden ocasionar múltiples infecciones en diferentes órganos y sistemas de órganos. Objetivo: resumir los aspectos más novedosos relacionados con las Chlamydias en cuanto a sus características y formas de evitarla.Métodos: se realizó una revisión bibliográfica sobre la importancia clínica de las Chlamydias en la especie humana y su elevada incidencia. Se consultó la base de datos Medline disponible en INFOMED, de donde se extrajeron referencias bibliográficas entre los años 1995 y 2011, acerca de algunos aspectos etiológicos, clínicos, diagnósticos y terapéuticos de acuerdo a los últimos apuntes más relevantes en la literatura médica sobre Chlamydias y su relación con el hombre. Resultados: la Chlamydia Trachomatis en particular responsable del tracoma, primera causa de ceguera prevenible, provoca un impacto deletéreo en la salud reproductiva, produciendo entidades como abortos espontáneos, rotura prematura de membranas, restricción del crecimiento intrauterino, endometritis, conjuntivitis y neumonía del recién nacido. Es responsable además de Linfogranuloma Venéreo, Uretritis no Gonocóccica, Síndrome de Reiter, Enfermedad Inflamatoria Pélvica, Síndrome de Fitz Hugh Curtis, Embarazo Ectópico Crónico, Dolor Pélvico Crónico y más recientemente Carcinoma Cervical, entre otras. Conclusiones: partiendo de la base del impacto social y económico que tiene la infección por Chlamydias en el mundo y en nuestro país, pudimos resumir los aspectos más novedosos en cuanto a los tipos, infecciones que causan y sus características, diagnóstico, terapéutica y medidas generales para evitarlas o reducir su impacto negativo en la sociedad...

Chlamydia Infections have reached high incidence in the world. They are important pathogens for both animals and man. In humans, they can cause multiple infections in different organs and organ systems. Objective: to summarize the most novel aspects related to Chlamydias with regard to their characteristics and ways to avoid them.Methods: a bibliographic review on the clinical importance of Chlamydias and their high incidence in humans was conducted. The MEDLINE database available in INFOMED was consulted; from which bibliographic references were taken between the years 1995 and 2011 about some etiologic, clinical, diagnostic and therapeutic aspects in relation to the latest relevant notes appearing in the medical literature about Chlamydias and their relation with the man. Results: Chlamydia Trachomatis, in particular, the responsible for trachoma and the first cause of preventable blindness, causes a deleterious impact on reproductive health, leading to entities such as spontaneous abortions, premature rupture of membranes, intrauterine growth restriction, endometritis, conjunctivitis, and pneumonia in the newborn. It is also responsible for Lymphogranuloma Venereum (LGV), Nongonococcal Urethritis (NGU), Reiter's syndrome, Pelvic Inflammatory Disease, Fitz-Hugh-Curtis Syndrome, Chronic Ectopic Pregnancy, Chronic Pelvic Pain, and most recently, Cervical Carcinoma, among others.Conclusions: on the basis of social and economic impact that the infections by Chlamydias have in the world and in our country, we could summarize the most recent aspects in relation to types, infections that they cause and their characteristics, diagnosis, therapy and general measures to avoid them or reduce their negative impact on society...