The Potential Mechanism of Hippo Signaling Pathway and Its Related miRNA Intervention in Alzheimer’s Disease and Parkinson’s Disease / 生物化学与生物物理进展

The main characteristics of neurodegenerative diseases represented by Alzheimer’s disease (AD) and Parkinson’s disease (PD) is the progressive irreversible loss of neurons, leading to varying degrees of pathological changes and loss of cognitive function. There is still no effective treatment. With the acceleration of global aging society, the incidence of neurodegenerative diseases is rapidly increasing, becoming a serious global public health concern that urgently requires the development of effective therapeutic strategies. The Hippo signaling pathway, a highly evolutionarily conserved pathway, consists of the core components MST1/2, LATS1/2, and downstream effectors, transcriptional co-activators YAP and TAZ. It plays a crucial role in the regulation of various biological processes such as cell proliferation, differentiation, development, and apoptosis. Dysregulation of the Hippo pathway contributes to the development of many diseases, including cancer, cardiovascular diseases, immune disorders, etc. Therefore, targeting the dysregulated components of the Hippo pathway may be an effective strategy for treating various diseases. Increasing evidence indicates that the Hippo pathway is excessively activated in the development of neurodegenerative diseases, manifested by increased expression of MST1 and downregulation of YAP. Stabilizing the Hippo pathway levels has shown improvements in AD and PD. However, most studies on the Hippo pathway in AD and PD focus on changes in the expression levels of Hippo pathway components, and research in other neurodegenerative diseases is still lacking. Therefore, further investigation is needed to fully understand the mechanistic role of the Hippo pathway in neurodegenerative diseases. Meanwhile, miRNA, similarly dysregulated in neurodegenerative diseases and serving as biomarkers, is a primary target for miRNA therapy in neurodegenerative diseases, including AD and PD. Activating or inhibiting dysregulated miRNAs is the main strategy of miRNA therapy during the neurodegenerative disease development. Evidence suggests that the interaction between the Hippo pathway and miRNA can result in widespread biological effects and crosstalk in the occurrence of different types of diseases. However, studies on the interplay between the Hippo pathway and miRNA in neurodegenerative diseases are relatively scarce. In this paper, we predicted the miRNAs related to Hippo pathway through bioinformatics database, and further screened the miRNAs with crosstalk relationship with Hippo signaling pathway through experiments in combination with PubMed. Then, the mechanism of action of Hippo signaling pathway related miRNAs in AD and PD is further elucidated. It is reported that the Hippo pathway and its related miRNA may exert neuroprotective effects by reducing oxidative stress, improving neuroinflammation, stabilizing autophagy levels, maintaining neuronal mitochondrial function, and ameliorating blood-brain barrier dysfunction, thereby delaying the progression of AD and PD. However, research on miRNA directly regulating the Hippo pathway to improve AD and PD is limited, and observations of the Hippo pathway and its related miRNA in other neurodegenerative diseases are scarce. However, considering the regulatory relationship between the Hippo pathway and miRNA in multiple diseases and their respective roles in key mechanisms of neurodegenerative diseases, such as oxidative stress and neuroinflammation, the crosstalk between miRNA and the Hippo pathway holds a crucial regulatory role in the development of neurodegenerative diseases. Thus, the interaction pathways of the Hippo pathway and its related miRNA may be a pivotal avenue for exploring effective therapeutic strategies for neurodegenerative diseases in the future.

Association study between mammalian sterile 20-like kinase 1 SNPs and colorectal cancer / 安徽医科大学学报

Objective @#To explore the association between mammalian sterile 20-like kinase 1(MST1)gene poly- morphism and haplotype and the risk of colorectal cancer,rectal cancer,and colon cancer in the Han population in Baotou area by case-control association study@*Methods @#A total of 390 patients with colorectal cancer diagnosed by pathology and 413 normal physical examination population were collected,and 2 ml of peripheral blood was taken for subsequent gene genotyping.Single nucleotide polymorphisms(SNPs)of MSTI gene were screened according to the genetic polymorphism data of Chinese Han population provided by the National Center for Biotechnology Information-Haplotype Mapping database.Gene genotyping was performed by Taqman method.Logistic regression was used to calculate the association between each SNP and the risk of colorectal cancer,colon cancer,and rectal cancer under codominant,dominant,overdominant,and recessive genetic models.@*Results @#Four SNPs of MSTI gene were screened,namely rs8000,rs2234197,rs2267853,and rs6073629.Among them,SNP rs2234197 was associated with the risk of rectal cancer.Compared with the GG+AA genotype,the AG genotype could reduce the risk of rectal cancer, OR[95%confidence interval(CI)]=0.657(0.442-0.976).SNP rs8000 was associated with the risk of colon cancer.Compared with the TT+GT genotype,theGG genotype could reduce the risk of colon cancer [OR(95%CI)=0.425(0.182, -0.992)].@*Conclusion @#MSTI gene SNP rs2234197 AG genotype and SNP rs8000 GG genotype may be protective factors for rectal cancer and colon cancer,respectively.

Uma nova variante de splicing STK3/MST2 que exibe atividade de quinase reduzida e potencial clínico / A novel STK3/MST2 splicing variant displays impaired kinase activity and clinical potential

A via Hippo consiste em uma cascata de serina-treonina quinases que desempenha um papel central na transdução de sinais mecânicos. Em mamíferos, o eixo canônico da via consiste na ativação das quinases MST1 e MST2 (codificadas pelos genes STK4 e STK3, respectivamente) e LATS1 e LATS2. A ativação dos dois últimos culmina na fosforilação, retenção citoplasmática e inativação dos coativadores transcricionais YAP e TAZ. A inativação de Hippo resulta na localização nuclear de YAP/TAZ, aumento da proliferação e contribui para a transformação maligna em células epiteliais. No presente trabalho, identificamos que o exon 7, que codifica um segmento do domínio quinase de MST2, estava ausente em células malignas da glândula mamária humana, T4-2, mas não na linhagem não maligna, S1. A exclusão do exon 7 compromete a interação de MST2 com MOB1, um dos principais substratos de MST2. Ao contrário da proteína completa, a superexpressão de MST2 sem o exon 7 não resultou em aumento da morte celular, bem como, não diminuiu a proliferação celular. Esta nova variante de STK3/MST2, a qual denominamos STK3Δ7/MST2Δ7 é produto de um exon skipping e foi encontrada em amostras de tumores de pacientes, mas pouco predominante em amostras de tecidos normais. Além disso, em pacientes com câncer pancreático, a expressão STK3Δ7 resultou em menor sobrevida específica da doença. A retenção do exon 7 foi menor em tumores mais agressivos e com alto grau histológico. Em ensaio 3D, células não malignas com expressão ectópica de MST2Δ7 não respondem aos sinais inibitórios da membrana basal reconstituída e formam estruturas tumor-like. Esta nova variante perde sua atividade quinase e pode perturbar a homeostase tecidual pela incapacidade de ativar morte e inibir a proliferação celular, mesmo em microambientes repressores desses processos em células normais, como na presença membrana basal. Esses achados podem avançar o nosso conhecimento sobre progressão tumoral com possível relevância clínica

The Hippo pathway consists of a cascade of serine-threonine kinases that plays a central role in the transduction of mechanical signals. In mammals, the canonical axis of the pathway consists of the activation of the kinases MST1 and MST2 (encoded by the genes STK4 and STK3, respectively) and LATS1 and LATS2 and their activation culminates in the phosphorylation, cytoplasmic retention and inactivation of the transcriptional coactivators YAP and TAZ. Hippo inactivation results in nuclear localization of YAP/TAZ, increased cell proliferation, and contributes to malignant transformation in epithelial cells. In the present work, we identified that exon 7, which encodes a segment of the kinase domain of MST2, was absent in malignant cells of the human mammary gland, T4-2, but not in the non-malignant S1 cell line. Exclusion of exon 7 compromises the interaction of MST2 with one of its main substrates, MOB1. Unlike the full-length protein, overexpression of MST2 without exon 7 did not result in increased cell death, nor decreased cell proliferation. This new variant of STK3/MST2, which we named STK3Δ7/MST2Δ7, is the product of an exon skipping and was found in tumor samples, but seldomly found in samples of normal tissues. Furthermore, in patients with pancreatic cancer, STK3Δ7 expression resulted in lower disease-specific survival. Exon 7 retention was reduced in aggressive tumors with a high histological grade. In a 3D assay, non-malignant cells with ectopic expression of MST2Δ7, even at low concentrations, do not respond to inhibitory signals from a reconstituted basement membrane and form tumor-like structures. This new variant loses its kinase activity and may disturb the tissue homeostasis due to its inability to activate death and to inhibit cell proliferation, even in microenvironments that repress these processes in normal cells, such as the basement membrane. These findings may advance our knowledge about tumor progression and might be clinically relevant

Uma análise do projeto de educação nos documentos do MST (1990-2014): a cultura corporal em debate / n analysis of the education project in the MST documents (1990-2014): corporal culture in debate / Un análisis del proyecto educativo en los documentos del MST (1990-2014): cultura corporal en debate

No presente estudo, referenciamos o debate sobre Educação e Movimentos Sociais, através das contribuições do Movimento dos Trabalhadores Rurais Sem Terra (MST). Desse modo, buscamos apresentar mediações entre o Projeto de Educação do MST e a Cultura Corporal, a partir do seguinte problema: Se e como a Cultura Corporal está retratada no projeto de Educação do MST? O objetivo é caracterizar a concepção de Cultura Corporal com base nos textos/documentos sobre Educação sistematizados pelo MST. Para subsidiar a análise, trazemos as contribuições teóricas de Antonio Gramsci, sobre cultura, princípio educativo, escola unitária e o conceito de intelectual, bem como o Coletivo de Autores sobre a perspectiva de Cultura Corporal (AU).

In the present study, we aim to the debate on Education and Social Movements, through the contributions of the Landless Rural Workers Movement (MST). In this way, wes eek to present mediations between the MST Education Project and Corporal Culture, base don't he following problem: I fan dhow is Corporal Culture portrayed in the MST education project? The object ivies to characterize the concept of Corporal Culture from the texts/documents on education systematized by the MST. To support he analysis, we bright et he oretical contributions of Antonio Gramsci, on culture, educational principle, unitary school and the concept of intellectual, as well as the Coletivo de Autores on the perspective of Corporal Culture (AU).

En este estudio nos referimos al debate sobre Educación y Movimientos Sociales, a través de los aportes del Movimiento de Trabajadores Rurales Sin Tierra (MST). Así, buscamos presentar mediaciones entre el Proyecto Educativo MST y la Cultura Corporal, partiendo del siguiente problema: ¿Si y cómo se retrata la Cultura Corporal en el proyecto educativo MST? El objetivo es caracteriza el concepto de Cultura Corporal a partir de los textos/documentos sobre educación sistematizados por el MST. Para sustentar elanálisis, traemoslos aportes teóricos de Antonio Gramsci, sobre cultura, principio educativo, escuela unitaria y el concepto de intelectual, así como el Coletivo de Autores en la perspectiva de la Cultura Corporal (AU).

Uma análise do projeto de educação nos documentos do MST (1990-2014): a cultura corporal em debate / An analysis of the education project in the MST documents (1990-2014): corporal culture in debate / Un análisis del proyecto educativo en los documentos del MST (1990-2014): cultura corporal en debate

No presente estudo, referenciamos o debate sobre Educação e Movimentos Sociais, através das contribuições do Movimento dos Trabalhadores Rurais Sem Terra (MST). Desse modo, buscamos apresentar mediações entre o Projeto de Educação do MST e a Cultura Corporal, a partir do seguinte problema: Se e como a Cultura Corporal está retratada no projeto de Educação do MST? O objetivo é caracterizar a concepção de Cultura Corporal com base nos textos/documentos sobre Educação sistematizados pelo MST. Para subsidiar a análise, trazemos as contribuições teóricas de Antonio Gramsci, sobre cultura, princípio educativo, escola unitária e o conceito de intelectual, bem como o Coletivo de Autores sobre a perspectiva de Cultura Corporal.

In the present study, we aim to the debate on Education and Social Movements, through the contributions of the Landless Rural Workers Movement (MST). In this way, wes eek to present mediations between the MST Education Project and Corporal Culture, base don't he following problem: I fan dhow is Corporal Culture portrayed in the MST education project? The object ivies to characterize the concept of Corporal Culture from the texts/documents on education systematized by the MST. To support he analysis, we bright et he oretical contributions of Antonio Gramsci, on culture, educational principle, unitary school and the concept of intellectual, as well as the Coletivo de Autores on the perspective of Corporal Culture.

En este estudio nos referimos al debate sobre Educación y Movimientos Sociales, a través de los aportes del Movimiento de Trabajadores Rurales Sin Tierra (MST). Así, buscamos presentar mediaciones entre el Proyecto Educativo MST y la Cultura Corporal, partiendo del siguiente problema: ¿Si y cómo se retrata la Cultura Corporal en el proyecto educativo MST? El objetivo es caracterizar el concepto de Cultura Corporal a partir de los textos/documentos sobre educación sistematizados por el MST. Para sustentar el análisis, traemoslos aportes teóricos de Antonio Gramsci, sobre cultura, principio educativo, escuela unitaria y el concepto de intelectual, así como el Coletivo de Autores en la perspectiva de la Cultura Corporal.

Radix Scrophulariae Extracts Exert Effect on Hyperthyroidism via MST1/Hippo Signaling Pathway / 中国结合医学杂志

OBJECTIVE@#To explore the mechanism of Radix Scrophulariae (RS) extracts in the treatment of hyperthyroidism rats by regulating proliferation, apoptosis, and autophagy of thyroid cell through the mammalian sterile 20-like kinase 1 (MST1)/Hippo pathway.@*METHODS@#Twenty-four rats were randomly divided into 4 groups according to a random number table: control, model group, RS, and RS+Hippo inhibitor (XMU-MP-1) groups (n=6 per group). Rats were gavaged with levothyroxine sodium tablet suspension (LST, 8 μ g/kg) for 21 days except for the control group. Afterwards, rats in the RS group were gavaged with RS extracts at the dose of 1,350 mg/kg, and rats in the RS+XMU-MP-1 group were gavaged with 1,350 mg/kg RS extracts and 1 mg/kg XMU-MP-1. After 15 days of administration, thyroid gland was taken for gross observation, and histopathological changes were observed by hematoxylin-eosin staining. The structure of Golgi secretory vesicles in thyroid tissues was observed by transmission electron microscopy. The expression of thyrotropin receptor (TSH-R) was observed by immunohistochemistry. Terminal-deoxynucleoitidyl transferase mediated nick end labeling assay was used to detect cell apoptosis in thyroid tissues. Real-time quantity primer chain reaction and Western blot were used to detect the expressions of MST1, p-large tumor suppressor gene 1 (LATS1), p-Yes1 associated transcriptional regulator (YAP), proliferating cell nuclear antigen (PCNA), G1/S-specific cyclin-D1 (Cyclin D1), B-cell lymphoma-2 (Bcl-2), Caspase-3, microtubule-associated proeins light chain 3 II/I (LC3-II/I), and recombinant human autophagy related 5 (ATG5). Thyroxine (T4) level was detected by enzyme-linked immunosorbent assay.@*RESULTS@#The thyroid volume of rats in the model group was significantly increased compared to the normal control group (P<0.01), and pathological changes such as uneven size of follicular epithelial cells, disorderly arrangement, and irregular morphology occurred. The secretion of small vesicles by Golgi apparatus was reduced, and the expressions of receptor protein TSH-R and T4 were significantly increased (P<0.01), while the expressions of MST1, p-LATS1, p-YAP, Caspase-3, LC3-II/I, and ATG5 were significantly decreased (P<0.01). The expressions of Bcl-2, PCNA, and cyclin D1 were significantly increased (P<0.01). Compared with the model group, RS extracts reduced the volume of thyroid gland, improved pathological condition of the thyroid gland, promoted secretion of the secretory vesicles with double-layer membrane structure in thyroid Golgi, significantly inhibited the expression of TSH-R and T4 levels (P<0.01), upregulated MST1, p-LATS1, p-YAP, Caspase-3, LC3-II/I, and ATG5 expressions (P<0.01), and downregulated Bcl-2, PCNA, and Cyclin D1 expressions (P<0.01). XMU-MP-1 inhibited the intervention effects of RS extracts (P<0.01).@*CONCLUSION@#RS extracts could inhibit proliferation and promote apoptosis and autophagy in thyroid tissues through MST1/Hippo pathway for treating hyperthyroidism.

The hippo kinases MST1/2 in cardiovascular and metabolic diseases: A promising therapeutic target option for pharmacotherapy

Cardiovascular diseases (CVDs) and metabolic disorders are major components of noncommunicable diseases, causing an enormous health and economic burden worldwide. There are common risk factors and developmental mechanisms among them, indicating the far-reaching significance in exploring the corresponding therapeutic targets. MST1/2 kinases are well-established proapoptotic effectors that also bidirectionally regulate autophagic activity. Recent studies have demonstrated that MST1/2 influence the outcome of cardiovascular and metabolic diseases by regulating immune inflammation. In addition, drug development against them is in full swing. In this review, we mainly describe the roles and mechanisms of MST1/2 in apoptosis and autophagy in cardiovascular and metabolic events as well as emphasis on the existing evidence for their involvement in immune inflammation. Moreover, we summarize the latest progress of pharmacotherapy targeting MST1/2 and propose a new mode of drug combination therapy, which may be beneficial to seek more effective strategies to prevent and treat CVDs and metabolic disorders.

New possible silver lining for pancreatic cancer therapy: Hydrogen sulfide and its donors

As one of the most lethal diseases, pancreatic cancer shows a dismal overall prognosis and high resistance to most treatment modalities. Furthermore, pancreatic cancer escapes early detection during the curable period because early symptoms rarely emerge and specific markers for this disease have not been found. Although combinations of new drugs, multimodal therapies, and adjuvants prolong survival, most patients still relapse after surgery and eventually die. Consequently, the search for more effective treatments for pancreatic cancer is highly relevant and justified. As a newly re-discovered mediator of gasotransmission, hydrogen sulfide (H

A ciranda infantil na visão de crianças de um assentamento rural vinculado ao MST / The children’s ciranda by the view of children from a rural settlement linked to the landless workers’ movement (MST) / La ciranda infantil desde la mirada de niños de un asentamiento rural del MST

A pesquisa objetivou compreender as significações de crianças de um assentamento rural vinculado ao MST, na região de Ribeirão Preto-SP, acerca de um espaço/tempo especificamente a elas destinado, denominado Ciranda Infantil. Utilizamos como instrumentos diário de campo, fotografias realizadas pelas crianças, conversas baseadas em fotografias, fantoches. Participaram seis crianças de 10 a 12 anos. Os resultados indicam que a Ciranda tem sido significada pelas crianças como um espaço de socialização e aprendizagens variadas entre adultos e crianças, marcadas pela construção de relações afetivas, socioambientais e geracionais, com destaque para a ludicidade. Conclui-se que as crianças percebem que existe para elas um projeto intencional e apropriam-se desse espaço como sujeitos críticos que colaboram em sua construção e, ou, disputam-no com os adultos. Esse sentimento de ter a Ciranda como espaço próprio os autoriza a propor melhorias para sua realização.

This research aimed to understand the meanings yielded by children of a rural settlement linked to the Landless Workers’ Movement (MST), in the region of Ribeirão Preto (SP, Brazil), about a space/time assigned specifically to them, called Children’s Ciranda. As methodological instruments, we used field diary, photographs taken by the children; conversations based on the photographs; and puppets. Six children aged from ten to twelve years participated. The results indicate that "Ciranda" has been signified by children as a space of socialization and varied learnings among adults and children, marked by the construction of affective, socio-environmental and generational relations, highlighting playfulness. We conclude that children realize that there is an intentional project for them and they take property of this space as critical subjects who collaborate in its construction and / or compete with adults. This feeling of having the “Ciranda” as their own space authorizes them to propose improvements to its realization.

La investigación tuvo como objetivo comprender los significados de los niños de un asentamiento rural vinculado al MST, en Ribeirão Preto (SP), acerca de un espacio / tiempo destinado específicamente hacia ellos, llamado "Ciranda Infantil". Se utilizó como instrumentos: diario de campo; fotografías tomadas por los niños; conversaciones acerca de las fotografías y marionetas. Participaron seis niños de diez a doce años. Los resultados indican que la "Ciranda" ha sido significada por los niños como un espacio de socialización y aprendizajes variados entre adultos y niños, así como marcada por la construcción de relaciones afectivas, ambientales y generacionales, destacando su carácter lúdico. Llegamos a la conclusión de que los niños perciben que este espacio fue creado para ellos y se apropian de ello como sujetos críticos que colaboran en su construcción y / o la disputan con los adultos. Y esta sensación de tener la "Ciranda" como un espacio suyo les autoriza a proponer mejoras para su realización.

Oficinas com adolescentes do MST: sexualidade, diversidade sexual e gênero / Workshops with MST teenagers: sexuality, sexual diversity and gender / Talleres con adolescentes DEL MST: sexualidad, diversidad sexual y género

O objetivo do presente artigo é apresentar as oficinas sobre sexualidade, diversidade sexual e gênero, realizadas em uma escola de agroeocologia do Movimento dos Trabalhadores Rurais Sem Terra (MST). Participaram 36 adolescentes e jovens, com idade entre 15 e 24 anos, da mesma turma do curso de agroecologia. A demanda que nos foi endereçada, tanto pelos educandos como pela coordenação da escola, foi trabalhar com o tema sexualidade. Realizamos 15 encontros semanais no ano de 2015, com 2 horas de duração, com os seguintes temas geradores: gênero e relacionamentos abusivos, diversidade sexual, infecções sexualmente transmissíveis e métodos contraceptivos. As técnicas utilizadas foram variadas: dinâmicas de grupo, mitos e verdades, exibição e discussão de filmes (curtas e longas). Nas oficinas, trabalhamos de uma forma dinâmica, esclarecendo dúvidas, proporcionando experiências e situações que estimulassem a reflexão e a expressão de vivências, para superar o enfoque do binômio saúde-doença.

The aim of this article is to present the workshops about sexuality, sexual diversity and gender, performed in an agroecology school of the Landless Workers' Movement (MST). The participants were 36 adolescents and young, aged 15 to 24 years, from the same class of the agroecology course. The demand addressed to us, both by the students and by the coordination of the school, was approaching the sexuality theme. We performed 15 weekly meetings during the year 2015, with the duration of 2 hours each, about the following generating themes: gender and abusive relationships, sexual diversity, sexually transmitted diseases and contraceptive methods. Different techniques were used, such as: group dynamics, myths and truths, exhibition and discussion of short and long films. In the workshops, we pursued a dynamical working method, clarifying doubts, providing experiences and situations that stimulate reflection and sharing of experiences, in order to overcome the health-disease binomial approach.

El objetivo del presente artículo es presentar los talleres sobre sexualidad, diversidad sexual y género, realizados en una escuela de agroecología del Movimiento de los Trabajadores Rurales Sin Tierra (MST). Los participantes fueron 36 adolescentes y jóvenes, con edades entre 15 a 24 años, de la misma clase del curso de agroecología. La demanda que nos ha sido dirigida, tanto por los alumnos como por la coordinación de la escuela, fue trabajar con el tema sexualidad. Realizamos 15 encuentros semanales en el año 2015, con 2 horas de duración, con los siguientes temas generadores: género y relación abusiva, diversidad sexual, enfermedades sexualmente transmisibles y métodos anticonceptivos. Las técnicas utilizadas fueron variadas: dinámica de grupo, mitos y verdades, exhibición y discusión de películas (corto y largometraje). En los talleres, propusimos trabajar de un modo dinámico, aclarando dudas, proporcionando vivencias y situaciones para fomentar la reflexión y la expresión de experiencias, buscando superar el enfoque del binomio salud-enfermedad.

Clinical audit on adherence to using Malnutrition Screening Tool and dietitian referral in the Oncology Outpatient Clinic, National Cancer Institute, Malaysia

@# Introduction: Malnutrition is a frequent complication in cancer patients and can negatively affect treatment outcome. Preliminary audit conducted at the Oncology Clinic, National Cancer Institute (NCI), found that only 5.8% of outpatients underwent nutrition screening using the Malnutrition Screening Tool (MST), and only 2.6% of dietitian referrals were recorded. This audit aims to determine the rate of adherence to nutritional screening, and to implement remedial measures for improved patient care. Methods: This was a cross-sectional audit comprising three phases, namely initial audit, remedial measures and re-audit. Criteria audited include screening rate using MST and dietitian referral based on MST scores. Standards were set at 100% for both criteria. Data collected for initial audit were patients’ MST scores records and total dietitian referral forms retrieved from Electronic Medical Records. After initial audit, self-administered questionnaires for nurses and physicians were developed to identify barriers. Measures implemented for change included patientadministered MST to shorten screening time, and procedure flowchart to facilitate referral. After 6 months, a re-audit was conducted. Results: Total subjects for initial audit and re-audit were 349 and 390, respectively. Initial audit and re-audit showed screening rate using MST increased significantly from 6.3% to 79.5%, but there was no significant change for the dietitian referral rate. Conclusion: This clinical audit has led to a change in the policy in NCI outpatient clinics whereby nurses directly schedule dietitian referrals without going through physicians for patients with MST scores ≥2. Continuous audit and monitoring are necessary to facilitate improvement in MST implementation for better outpatient care.

Oficina com adolescentes do MST: preconceito e convivência coletiva / Workshop with MST teenagers: prejudice and social coexistence / Taller con adolescentes del MST: prejuicio y convivencia colectiva

Apesar de todas as conquistas dos movimentos sociais no que se refere à educação do campo, as crianças e jovens do Movimento dos Trabalhadores Rurais Sem-Terra (MST) vivenciam situações de preconceito por sua pertença, quando estudam em escolas não vinculadas ao movimento, vivencias que os marcam profundamente. O preconceito pela pertença ao MST que vem a se somar a diversos outros, foi o tema demandado para ser trabalhado em nosso projeto de extensão, realizado em uma escola técnica de agroecologia fruto de uma parceria entre o MST e o Instituto Federal Tecnológico do Paraná (ITFPR). Os participantes do projeto foram adolescentes e jovens, com idades que variam entre 15 e 24 anos, sete são do sexo feminino e os demais do sexo masculino, todos oriundos de acampamentos e assentamentos da reforma agrária. A metodologia adotada no trabalho foi a realização de oficinas com o tema principal "preconceito e convivência coletiva" e os seguintes temas geradores: possibilidades e limites frente às diferenças, preconceito e empatia, empatia e comunicação, preconceito racial e bullying. Foram realizados oito encontros semanais, com duração de 2 h. Concluímos que a metodologia das oficinas se mostrou efetiva ao ir além dos aspectos educativos/informativos, abarcando e trabalhando com os significados afetivos, os sentimentos e as vivências, proporcionando espaço para a fala, a reflexão e a elaboração, tanto individual como coletiva. Além de potencializar o contexto favorável a formação humana que o MST construiu ao longo da sua existência.

Despite all the achievements of social movements in rural education, the children and youth of the Landless Workers' Movement (MST) experience situations of prejudice because of their belonging when they study in schools not linked to the movement, experiences that profoundly mark them. The prejudice for belonging to the MST that comes to be added to several other, was demanded as a theme to be worked on our extension project, developed in a technical school of agroecology, which is a result of a partnership between the MST and the Federal Technological Institute of Paraná (ITFPR). The participants of the project were teenagers and young people, ages ranging between 15 and 24 years, 7 are women and 23 are men; all of them were from camps and settlements ofagrarian reform. The methodology adopted in the work was performing workshops about the main theme "prejudice and social coexistence" and with the following emerged topics: possibilities and limits in face of differences, prejudice and empathy, empathy and communication, racial prejudice and bullying. Eight weekly meetings were held, lasting 2 hours. We concluded that the methodology of the workshops was effective when going beyond the educational/informational aspects, embracing and working with affectivemeanings, feelings and experiences, providing space for speech, reflection and elaboration, both individual and collective, and also enhancing the favorable context to human formation that the MST has built throughout its existence.

A pesar de todas las conquistas de los movimientos sociales en relación a la educación rural, los niños y jóvenes del Movimiento de los Sin Tierra (MST) viven situaciones de prejuicio por su pertenencia cuando estudian en las escuelas no vinculados al movimiento, esas vivencias los marcan profundamente. El perjuicio por pertenecer al MST, que viene a añadirse a muchos otros, fue el tema demando para el estudio en nuestro proyecto de extensión, realizado en una escuela técnica de agroecología fruto de una asociación entre el MST y el Instituto Tecnológico Federal de Paraná (ITFPR). Los participantes del proyecto son adolescentes y jóvenes, con edades entre 15 y 24 años, 7 son del sexo femenino y los demás, 23, del sexo masculino, todos son oriundos de campamentos y asentamientos de la reforma agraria La metodología utilizada en el estudio fueron los talleres con el tema principal "los prejuicios y la convivencia" y los siguientes temas generadores: posibilidades y límites frente a las diferencias, al prejuicioy a la empatía; empatía y comunicación; prejuicio racial y bullying.. Se realizaron 8 citas semanales, con duración de 2h. La conclusión es que la metodología de los talleres demostró ser eficaz para ir más allá de los aspectos educativos / informativos, incluyendo y trabajando con los significados afectivos, los sentimientos y las experiencias, proporcionando espacio para el habla, la reflexión y el desarrollo, tanto individuales como colectivas. Además de potenciar el entorno favorable a la formación humana que el MST ha construido a lo largo de su existencia.

A Educação Física no contexto da educação do campo: a realidade do conteúdo jogo na escola / Physical Education in the context of rural education: the reality of the content 'game' in school / Educación Física en el contexto de la educación do campo: la realidad del contenido del juego en la escuela

O presente trabalho é resultado do Projeto de Pesquisa "A Educação Física no Movimento dos Trabalhadores Rurais Sem Terra (MST): experiências pedagógicas a partir da Cultura Corporal". O estudo foi realizado em áreas de reforma Agraria do MST na região do Recôncavo da Bahia. O objetivo geral foi analisar a realidade da prática pedagógica do jogo, enquanto conteúdo de ensino da Educação Física, na Escola do Assentamento Nova Panema, no período de 2009/2011. A metodologia da pesquisa utilizou os instrumentos da observação participante, registros fotográficos e de campo, analise de documentos e entrevista semi-estruturada. Os resultados evidenciam que, apesar da Educação Física não constar como componente curricular no projeto político-pedagógico da escola, o conteúdo "jogo" estava presente na prática pedagógica da professora da turma multisseriada. Contudo, este vinha sendo utilizado na escola predominantemente como um instrumento de ensino ou como atividade recreativa.

This paper presents the results of the research "Physical Education in the Landless Workers' Movement (MST): pedagogical experiences". The study was conducted in agrarian reform areas of the MST in the Recôncavo region, State of Bahia, Brazil. Its overall aim was was to analyze the reality of the pedagogical practice of the content game in Physical Education classes at the School of the Settlement New Panema, between the years 2009 until 2011. Data collection instruments used in this research consisted of the literature review, participant observation, analysis and semi-structured interviews. The results reveal that despite the physical education not having appear as a curricular component in the political-pedagogical project of the school, the content "game" was present in the pedagogical practice of multisseriate class. However, this was used only as a teaching tool for other fields of knowledge or as a recreational activity; not as a content of Physical Education classes.

Este artículo presenta los resultados de la investigación "Educación Física en el Movimiento de los Sin Tierra (MST): experiencias de enseñanza". El estudio se realizó en áreas de reforma Agraria del MST en la región de Recôncavo de Bahía, Brasil. El objetivo fue analizar la realidad de la práctica pedagógica del juego, en la enseñanza de contenidos de la Educación Física en la Escuela Nueva Panema, en el periodo 2009/2011. La metodología de investigación utilizo la observación participante, registros fotográficos, análisis de documentos y la entrevista semiestructurada. Los resultados muestran que la educación física no es un componente curricular en el proyecto político-pedagógico de la escuela. Sin embargo, el contenido juego estuvo presente en la práctica pedagógica del maestro de la clase multiseriada y estaba siendo utilizado en la escuela como una herramienta de enseñanza de otros campos de conocimiento o como una actividad recreativa.

Educação, gestão social e desenvolvimento local: algumas considerações sobre a experiência do MST na luta pela gestão social da escola / Education, social management and local development: some thoughts on the experience of MST in the fight for school social management / Educación, gestión social y desarrollo local: algunas reflexiones sobre la experiencia del MST en la lucha por la gestión social de la escuela

Gestão social e desenvolvimento local são categorias cujos sentidos e finalidades podem ser "a favor do social" ou "contra o social". Tais categorias configuram perspectivas de análise da realidade diferentes, bem como perspectivas de projetos societais divergentes. Partindo desse pressuposto, o texto apresenta a análise sobre a relação entre educação-desenvolvimento local e gestão social da escola na experiência do Movimento dos Trabalhadores Rurais Sem Terra (MST) em Minas Gerais. Resulta de pesquisa realizada no Doutorado¹ no período de 2009 a 2011 e compreendeu uma abordagem qualitativa, com entrevistas, aplicação de questionários e observação de atividades desenvolvidas nas escolas pesquisadas e no assentamento em geral. A análise realizada leva a concluir que, no espaço da implantação do seu projeto de educação, existe uma disputa entre uma concepção de gestão social da escola e da educação defendida pelo MST e o modelo gerencial do Estado, o que se configura como um desafio a ser superado pelo Movimento.

Social management and local development are categories whose senses and purposes can be "in favor of the social" or "against social". These categories constitute analytical perspectives of different realities and perspectives of different societal projects. Based on this assumption, the text presents the analysis of the relationship between local education-development and school management in the social movement of the experience of the Landless Rural Workers (MST) in Minas Gerais. The text results of research conducted in Doctorate from 2009 to 2011; it comprised a qualitative approach with interviews, questionnaires and observation activities in the schools surveyed and in settlement in general. The analysis leads to the conclusion that within the implementation of its education project, there is a dispute between a conception of social management of the school and the education advocated by the MST and the management model of the state, which is configured as a challenge to be overcome by the Movement.

La gestión social y el desarrollo local son categorías cuyos sentidos y propósitos pueden ser "a favor de lo social" o "contra la exclusión social". Estas categorías constituyen perspectivas de análisis de diferentes realidades y perspectivas de los diferentes proyectos sociales. Con base en este supuesto, el texto presenta el análisis de la relación entre educación-desarrollo local y la gestión escolar en el movimiento social de la experiencia de la Trabajadores Rurales Sin Tierra (MST) en Minas Gerais. El texto resulta de la investigación realizada en Doctorado de 2009 hasta 2011 y se compone de un enfoque cualitativo con entrevistas, cuestionarios y actividades de observación en las escuelas encuestadas y asentamiento en general. El análisis lleva a la conclusión de que en la ejecución de su proyecto de educación, hay una disputa entre una concepción de la gestión social de la escuela y la educación defendida por el MST y el modelo de gestión del Estado, que se configura como un desafío a ser superado por el Movimiento.

Fatores determinantes de conhecimentos, atitudes e práticas em DST/Aids e hepatites virais, entre jovens de 18 a 29 anos, no Brasil / Determinant factors of knowledge, attitudes and practices regarding STD/AIDS and viral hepatitis among youths aged 18 to 29 years in Brazil / Facteurs déterminants des connaissances, des attitudes et des pratiques en MST/SIDA et hépatites virales parmis les jeunes âgés de 18 à 29 ans au Brésil

Resumo O objetivo deste estudo foi avaliar a vulnerabilidade dos jovens brasileiros às DSTs/Hiv Aids e Hepatites Virais. Foram entrevistados 1.208 jovens (18 e 29 anos em 15 Estados e DF). A margem de erro da pesquisa ponderada regional e nacionalmente foi 2,8%. O estudo foi aprovado pelo CEP da Faculdade de Medicina da UnB e teve apoio da OPAS e do Ministério da Saúde. Escala com 35 questões (-35 a +35 pontos) de conhecimentos, atitudes e práticas em DSTs/Hiv Aids e Hepatites Virais foi utilizada como variável dependente. Modelos de regressão linear ajustados identificaram fatores demográficos e determinantes sociais que explicam a variação na escala. A escala atingiu nível satisfatório de consistência (Alfa Cronbach: 0,689). Fatores sociodemográficos associados à variação na escala foram: gênero, etnia, escolaridade e estado civil. Determinantes sociais associadas à variação na escala foram: hábito de conversar sobre sexualidade com pais e profissionais de saúde, consumo de álcool, ter lazer e ser engajado, acesso à internet, interesse em aprender e ter pai e/ou professor como referência pessoal. Os jovens brasileiros estão vulneráveis à transmissão de DSTs/Hiv Aids. São necessárias políticas públicas que promovam engajamento dos pais e professores em assuntos sobre sexualidade.

Abstract The scope of this study was to evaluate the vulnerability of Brazilian youths to STDs/HIV&AIDS and Viral Hepatitis. Interviews were conducted with 1,208 youths aged 18 to 29 in 15 states and the Federal District. The regional and national adjusted margin of error of the research was 2.8%. The study was approved by the School of Medicine of Brasilia University with support from PAHO and the Ministry of Health. A scale with 35 questions (knowledge, attitudes and practices) was the main dependent variable. Adjusted linear regression models identified the demographic and social determinant factors that explain scale variations. The scale attained a satisfactory level of consistency (Cronbach's Alpha: 0.689). Sociodemographic factors associated to scale variations include gender, race, education and civil status. Social determinants associated to scale variations include frequency of discussions about sexuality with parents and health professionals, alcohol consumption, leisure and being part of a social movement, access to the internet, interest in learning and having the father and/or teacher as a personal reference. Brazilian youths are vulnerable to the transmission of STDs/HIV&AIDS. Public policies are needed to promote the engagement of parents and teachers in issues related to sexuality.

Résumé L'objectif de cette étude a été d'évaluer la vulnérabilité des jeunes brésiliens aux MST/VIH SIDA et aux Hépatites Virales. À cet effet, 1.208 jeunes (âgés de 18 à 29 ans dans 15 États brésiliens et le DF [Distrito Federal] ont été interviewés. La marge d'erreur de la recherche pondérée régionalement et nationalement était de 2,8%. L'étude a été approuvée par le CEP [Comité d'Éthique en Recherche] de la Faculté de Médecine de l'UnB et a eu le support de l'OPAS [Organisation Panaméricaine de la Santé] et du Ministère de la Santé. Une échelle de 35 questions (-35 à +35 points) évaluant les connaissances, les attitudes et les pratiques en MST/VIH SIDA et Hépatites Virales a été utilisée comme une variable dépendante. Des modèles de régression linéaire ajustés ont identifié les facteurs démographiques et les déterminants sociaux qui expliquent la variation dans l'échelle. L'échelle a atteint un niveau satisfaisant de consistance (Alpha de Cronbach: 0,689). Des facteurs socio-démographiques associés à la variation dans échelle ont été pris en compte, à savoir: le genre, l´ethnie, la scolarité et l´ état civil. Les déterminants sociaux associés à la variation dans l'échelle: l´habitude de discuter sur la sexualité avec les parents et des professionnels de la santé, la consommation d'alcool, profiter du loisir et être engagé, avoir accès à Internet, avoir intérêt à apprendre et avoir un parent et/ou un enseignant comme référence personnelle.

Contralateral head leading turning accompanied by ipsilateral eye staring in a patient with seizure onset from posterior inferior temporal sulcus, a SEEG case report

The epileptic eye and head movements during epileptic seizures may be much more complicated thanpeople originally understood, which can be ipsilateral or contralateral to the electroencephalographyfocus. Here, we describe a male patient with drug resistant focal seizures associated with a directionalseparation between head and eye movement before evolving into generalized tonic-clonic seizure.His contralateral head leading turning showed forced, sustained, and unnatural features companiedby ipsilateral eye staring. Stereoeletroencephalography monitoring was performed, and 4 habitualseizures were recorded over 5 days. Three seizures showed left head leading turning and generalizedtonic-clonic seizure, and only one showed dizziness and ringing in the ears. All the seizures showedthat the ictal onset contacts were located in the posterior inferior temporal sulcus which borders onthe anterolateral part of medial superior temporal area. The patient underwent a resection includingtemporooccipital region, and the histopathology showed focal cortical dysplasia type Ic. He has beenseizure free for two years after operation. The scores of the intelligence and memory quotient improvedhalf year after operation.

Expression and clinical significance of MST1 in cervical cancer / 天津医药

Objective To investigate the expression and clinical significance of mammalian sterile 20-like kinase 1 (MST1) in cervical cancer. Methods Immunohistochemical method was applied to detect the expression level of MST1 protein in specimens of cervical cancer tissues (n=139) and pericarcinomatous tissues (n=20, with≥4 cm distance from the primary tumor's edge). Western blot assay and qPCR were used to detect the protein and mRNA transcription expression levels of MST1 in 20 pairs of cervical cancer tissues and pericarcinomatous tissues, respectively. The correlation between MST1 expression, clinic pathological features and the prognosis were analyzed. Results MST1 was mainly expressed in cytoplasm. The positive expression rate of MST1 was significantly lower in cervical cancer tissues (27%, 38/139) than that in pericarcinomatous tissues (80%, 16/20,χ2=21.62, P<0.01). The expressions levels of MST1 protein and mRNA were both lower in the cervical cancer tissues (P<0.01). In cervical cancer, the positive expression rate of MST1 inⅠb+Ⅱa stage was higher than that ofⅡb+Ⅳstage (P<0.05), the positive expression rate of MST1 in lymph node metastasis was lower than that of without lymph node metastasis (P < 0.05). Values of age, tumor size, histological type and differentiation degree showed no significant difference to positive expression rate of MST1. Moreover, the negative expression of MST1 displayed a significantly poorer overall survival time than that of positive expression of MST1 (Log-rank χ2=28.35, P < 0.01). Conclusion MST1 shows a lower expression in cervical cancer, which may be a new target for clinical treatment and prognosis of cervical cancer.

A Saúde como Potência de Ação: uma análise do coletivo e de Comuna do Movimento dos Trabalhadores Rurais Sem Terra (MST) / Health as a Strength Action: an analysis of the collective and the Comunne of the Landless Rural Workers Movement (MST) / La Salud como Potencia de Acción: un análisis del colectivo y de Comuna del Movimiento de los Trabajadores Rurales Sin Tierra (MST) / La Santé Comme Puissance D'action: une analyse du collectif et Commune du Mouvement des Travailleurs Ruraux Sans Terre (MST)

O texto apresenta resultados de pesquisa ação participante com o objetivo de colaborar com os esforços do MST na promoção da saúde de seus trabalhadores. A perspectiva de saúde adotada é a da totalidade dialético-materialista, ressaltando a sua dimensão ético política, cujo norte é a concepção espinosista de potência de vida. O foco da pesquisa são as afecções sofridas em uma "Comuna da Terra" do MST. Foram realizadas 49 entrevistas e elaborado diário de campo. Uma unidade de sentido se destacou na análise desses registros: a relação entre saúde e terra, seguida da organização comunitária e ações políticas. A não regularização da terra gera sofrimento psicossocial continuado, um conjunto de prejuízos individuais e coletivos que impedem o desenvolvimento de vínculos comunitários e a expansão da saúde. Ressalta-se o risco da maioria relegar o cuidado de si, com um corpo que clama por atenção.

This manuscript presents results from a participatory action research aimed to collaborate with the MST efforts to promote health for its workers. The concept of health here adopted refers to the dialectical-materialist as a totality, emphasizing its ethical-political perspective, based on Spinoza's concept concerning the force of life. The disorders occurred in Land Commune are the focus of this study. A total of 49 interviews were carried out and a field diary was elaborated. A meaningful unit was clear when analyzing these records: the correlation between health and land, followed by communitary organization and political actions. The non-regularization of land ownership leads to a continued psychosocial pain, a range of individual and collective damages that hampers the development of community bonds and the health expansion. It must be observed that most of them relegate taking care of themselves as if they were claiming for help.

El texto presenta resultados de investigación acción participante con el objetivo de colaborar con los esfuerzos del MST en la promoción de la salud de sus trabajadores. La perspectiva de salud adoptada es la de la totalidad dialéctica-materialista, resaltando su dimensión ético política, cuyo norte es la concepción espinosista de potencia de vida. El foco de la investigación son las afecciones sufridas en una "Comuna de la Tierra" del MST. Se realizaron 49 entrevistas y elaborado diario de campo. Una unidad de sentido se destacó en el análisis de esos registros: la relación entre salud y tierra, seguida de la organización comunitaria y acciones políticas. La no regularización de la tierra genera sufrimiento psicosocial continuado, un conjunto de pérdidas individuales y colectivas que impiden el desarrollo de vínculos comunitarios y la expansión de la salud. Se resalta el riesgo de la mayoría relegar el cuidado de sí, con un cuerpo que clama por atención.

Le texte présente les résultats de l'action des participants à la recherche dans le but de collaborer avec les efforts du MST pour promouvoir la santé de ses travailleurs. La perspective de la santé adoptée est celle de la totalité dialectique-matérialiste, en soulignant sa dimension politique éthique, dont le nord est la conception spinoziste de la puissance de la vie. L'objet de la recherche sont les affections subies dans une "Commune de la Terre" du MST. Au total, 49 entrevues ont été réalisées et un journal de bord a été préparé. Une unité de sens a été mise en évidence dans l'analyse de ces documents: la relation entre la santé et la terre, suivie de l'organisation communautaire et des actions politiques. La nonrégularisation de la terre génère une souffrance psychosociale continue, un ensemble de dommages individuels et collectifs qui entravent le développement des liens communautaires et l'expansion de la santé. Le risque de la majorité est de reléguer le soin de soi, avec un corps qui demande de l'attention.

Militância no Movimento dos Trabalhadores Rurais Sem Terra (MST): um olhar psicanalítico / Activism in the Landless Workers Movement (MST): a psychoanalytical view

Pretende-se trazer as contribuições da psicanálise para pensar a militância no Movimento dos Trabalhadores Rurais Sem Terra (MST) como práticas que ressignificam o passado e podem ser enriquecedoras para os sujeitos. Para tanto, apresentaremos as contribuições dos psicanalistas Arantes (1999) e Muldworf (2000) para o estudo da militância política e faremos uma breve caracterização do MST e de seus militantes. Destaca-se que a adesão a ideais sociais e lugar de prestígio que ocupa o militante no movimento lhe possibilita gratificação e revitalização narcísica e que estas não devem ser negligenciadas pelos movimentos sociais e nos estudos sobre a temática.

This article aims to bring contributions from psychoanalysis in order to address activism in the Brazilian Landless Workers Movement (MST) as a practice that reframes the past and can be fulfilling for subjects. To do so, we will present contributions from the psychoanalysts Arantes (1999) and Muldworf (2000) to the study of political activism, and briefly characterize the MST and its militants. We highlight that compliance with social ideals and the outstanding place the militant occupies within the movement allows for a narcissistic reward and revitalization, and that the latter should not be disregarded by social movements and studies on the theme.

Produção de Sentidos sobre a Militância Política de Mulheres Vinculadas ao MST / Production of Meanings about Political Militancy of Women Linked to the MST / La Producción de Significados sobre el Activismo Político de las Mujeres Vinculadas al MST / Production des Significations sur le Militantisme Politique des Femmes Liées au MST

O presente estudo objetivou investigar, por meio do aporte teórico do Construcionismo Social, a produção de sentidos sobre a militância política de mulheres vinculadas ao Movimento dos Trabalhadores Rurais Sem Terra (MST) no Rio Grande do Norte. Participaram do estudo seis mulheres que ocupavam as funções de coordenação estadual nas regiões de atuação do movimento. Utilizamos a entrevista semiestruturada como instrumento de produção de dados. As entrevistas foram analisadas a partir de uma categorização inicial do material e da construção de mapas de associação de ideias. Os sentidos em torno da militância política - produzidos nas associações por meio das expressões contribuição, esperança, reconhecimento, transformação, conscientização e luta ­ vinculam-se ao investimento coletivo de luta, não só pelo acesso à terra, mas por conquista de direitos sociais. Para o exercício de tal luta, o processo de formação política no MST empreende um importante papel, ao produzir repertórios articulados a uma identidade coletiva que, por sua vez, passa a ser vivida pelas mulheres no cotidiano das ações de militância. Os efeitos da militância na vida das participantes do estudo assinalam permanências e rupturas em relação ao papel feminino no âmbito familiar, seja na reprodução de um lugar socialmente designado às mulheres de cuidado da casa e da família, seja no questionamento desse lugar ao vivenciar graus de participação nas decisões familiares e de fomento à participação política, ao se inserirem em instâncias de representação política e institucional.

The present study aimed to investigate, through the theoretical contribution of Social Constructionism, the production of meanings about the political militancy of women linked to the Movement of Landless Rural Workers (MST) in Rio Grande do Norte. Six women who occupied the functions of state coordination in the regions of the movement participated in the study. We used the semi-structured interview as a data production instrument. The interviews were analyzed from an initial categorization of the material and the construction of maps of association of ideas. The senses surrounding political militancy - produced in associations through the expressions: contribution, hope, recognition, transformation, awareness and struggle - are linked to the collective investment of struggle, not only for access to land, but for the achievement of social rights. In order to carry out such a struggle, the process of political formation in the MST plays an important role, by producing repertoires articulated to a collective identity that, in turn, is experienced by women in the daily activities of militancy. The effects of militancy on the life of the study participants indicate that there is continuity and rupture in relation to the female role in the family sphere, either in the reproduction of a socially designated place of care for the home and family, or in the questioning of this place when experiencing degrees of participation in family decisions and fostering political participation, as they are inserted in instances of political and institutional representation.

Este trabajo objetivó investigar, por medio del aporte teórico del Construccionismo Social, la producción de sentidos sobre la militancia política de mujeres vinculadas al Movimiento de los Trabajadores Rurales Sin Tierra (MST) en el Rio Grande do Norte. Participaron de la investigación seis mujeres que ocupaban los puestos de coordinación estadual en las regiones de actuación del movimiento. Utilizamos la entrevista pre-estructurada como instrumento de producción de datos. Las entrevistas fueron analizadas partiendo de una categorización inicial del material y de la construcción de mapas de asociación de ideas. Los sentidos en vuelta de la militancia política ­ producidos en las asociaciones por medio de expresiones como contribución, esperanza, reconocimiento, transformación, concientización y lucha ­ están vinculados a la investida colectiva de lucha, no solo por el acceso a la tierra, pero por conquista de derechos sociales. Para el ejercicio de esta lucha, el proceso de formación política en el MST representa una importante función, al producir repertorios articulados a una identidad colectiva que, a su vez, pasa a ser vivida por las mujeres en el cotidiano de las acciones de militancia. Los efectos de la militancia en la vida de las participantes de la investigación señalan permanencias y roturas en relación a la función femenina en el ámbito familiar, sea en la reproducción de un sitio socialmente designado a las mujeres de cuidado con la casa y la familia, sea en el cuestionamiento de este sitio al vivir grados de participación en las decisiones familiares y de fomento a la participación política, al formar parte en locales de representación política e institucional.

Cette étude a eu l'objectif d'étudier, au moyen du cadre théorique du Constructivisme Social, la production de sens sur l'activisme politique des femmes liées au Mouvement des Travailleurs Sans Terre (MST) au Rio Grande do Norte. Six femmes qui travaillaient dans les coordinations régionnelles dans les zones d'action du mouvement ont participé de l'étude. Nous avons utilisé une interview semistructurée comme outil de production de données. Les interviews ont été analysées à partir d'une catégorisation initiale du matériel et de la construction des cartes d'association d'idées. Les sens autour du militantisme politique - produits dans les associations par des expressions suivantes: contribution, espoir, reconnaissance, transformation, sensibilisation et lutte, sont liés à l'investissement collectif de lutte, pas seulement pour l'accès à la terre, mais pour la conquête des droits sociaux. Pour l'exercice de cette lutte, le processus de formation politique chez le MST engage un rôle important, en produisant des répertoires articulés à une identité collective qui, à son tour, passe a être vécue par les femmes dans les actions militantes quotidiennes. Les effets du militantisme dans la vie des participants de l'étude indiquent les continuités et les ruptures par rapport au rôle des femmes dans la famille. Soit dans la réproduction d'un lieu socialement assigné aux femmes, c'est à dire, le soin de la maison et de la famille, soit la question de ce lieu à éxperimenter degrés de participation aux décisions de la famille et la promotion de la participation politique, quand elles sont inserées dans les instances de répresentations politiques et institutionnelles.