Determination of Resibufogenin and Cinobufagin in Meihuadianshe Pills by RP-HPLC / 中国药房

OBJECTIVE:To establish a RP-HPLC method for determination of the contents of resibufogenin and cinobufagin in Meihuadianshe pills METHODS:The condition of HPLC was Kromasil C18 column,acetonitrile-0 5%KH2PO4(50∶50) as a mobile phase and detecting wavelength 296nm RESULTS:This method showed good linearity The average recoveries of resibufogenin and cinobufagin were 97 40% with a RSD of 2 2% and 97 78% with a RSD of 2 6% respectively CONCLUSION:The method is simple,reliable and satisfactory in resolution It can be used in quality control of Meihuadianshe pills