Chemo-biological evaluation of antidiabetic activity of Mentha arvensis L. and its role in inhibition of advanced glycation end products

Background: There has been enormous curiosity in the development of alternative plant based medicinesto control diabetes, oxidative stress and related disorders. One of the therapeutic approaches is to reducepostprandial release of glucose in the blood. Two key enzymes that are involved in reducing postprandialglucose are a-amylase and a-glucosidase. Mentha arvensis L. has been traditionally used by several tribesas a medicinal plant to treat various disorders.Objective: The present study was undertaken to test M. arvenisis L. for inhibition of postprandialhyperglycemia.Material and method: We performed various in vitro and in vivo tests to evaluate efficacy of M. arvenisis L.for antidiabetic activity (postprandial hyperglycemia).Results: Methanolic extract of M. arvensis L. leaves showed DPPH free radical scavenging activity (morethan 78% mg/ml) and high antiglycation potential (more than 90% inhibition of AGE formation). Methanolic extract also showed remarkable inhibitory effects on a-amylase (more than 50% mg/ml) and aglucosidase (68% mg/ml) and significant inhibition of postprandial hyperglycemia in starch induced diabetic Wistar rats.Conclusion: The non-insulin dependent antidiabetic or inhibition of postprandial hyperglycemic activityof methanolic extract of M. arvensis L. leaves was shown by using in vitro and in vivo approaches in thepresent study.© 2018 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services byElsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L

Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract. In vivo analgesic activity was carried out by acetic acid-induced writhing test in Swiss albino mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was studied by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Brine shrimp lethality assay was used to investigate cytotoxicity effects of the plant extract. Results: The extract showed free radical scavenging activity in the DPPH assay (IC

Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L

Objective: To investigate potential antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. in different in vivo and in vitro experimental models. Methods: In vitro DPPH radical scavenging assay was used to evaluate the antioxidant activity of the plant extract. In vivo analgesic activity was carried out by acetic acid-induced writhing test in Swiss albino mice. All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight. Antibacterial activity was studied by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains. Brine shrimp lethality assay was used to investigate cytotoxicity effects of the plant extract. Results:The extract showed free radical scavenging activity in the DPPH assay (IC50~41 μg/mL) compared to the standard antioxidant ascorbic acid (IC50~19 μg/mL). The extract also produced prominent antimicrobial activity against Salmonella typhi, Salmonella paratyphi, Shigella boydii,Streptococcus pyogenes and Streptococcus aureus compared to standard drug kanamycin at the dose of 30 μg/disc. The extract exhibited lethality against the brine shrimp nauplii with the LC50 values of 40 μg/mL, and also 90% mortality (LC90) value was found to be 160 μg/mL. In analgesic test, the extract demonstrated statistically significant (P Conclusions:These results suggest that the ethanolic extract of Mentha arvensis L. has potential antioxidant, antibacterial, cytotoxic and analgesic activities that support the ethnopharmacological uses of this plant.

Tipos de estacas e substratos na propagação vegetativa da menta (Mentha arvensis L. ) / Types of cuttings and substrates in the vegetative propagation of mint (Mentha arvensis L. )

O trabalho teve como objetivo avaliar a propagação vegetativa da menta utilizando diferentes tipos de estacas e substratos. O experimento foi conduzido no Horto de Plantas Medicinais da Unimontes, campus Janaúba - MG. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 2 x 4 (dois tipos de estacas e quatro diferentes substratos) com quatro repetições, sendo cada parcela representada por seis estacas. Foram analisadas as variáveis comprimento de parte aérea e de raízes, massa seca de parte aérea e de raízes e número total de brotações formadas por planta. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Scott-Knott a 5% de probabilidade. A interação entre os fatores estacas e substratos não foi significativa para as variáveis estudadas, passando-se a estudar o efeito isolado de cada fator. A propagação de Mentha arvensis L. pode ser realizada tanto por estacas apicais como medianas, utilizando o substrato solo + areia + esterco bovino (2:1:1) para a produção de mudas de qualidade.

The purpose of the study was to evaluate the vegetative propagation using different types of mint cuttings and substrates. The experiment was conducted in the Garden of Medicinal Plants of Unimontes, in Janaúba - MG. The experimental design was completely randomized (CRD) in 2 x 4 factorial schemes (two types of poles and four different substrates) with four replications and each plot was represented by six cuttings. The variables analyzed were the length of the shoots and roots, the dry matter of the shoots and roots and the total number of shoots per plant. The data were subject to ANOVA and the means were compared by Scott-Knott's test at 5% of probability. The interaction among stem cuttings and substrates was not significant for the variables studied, thus, the isolated effect of each factor was studied. The propagation of Mentha arvensis L. can be performed either by apical cuttings as medians, using the substrate soil + sand + manure bovine (2:1:1) for the production of quality seedlings.

Produção de mudas de hortelã-japonesa em função da idade e de diferentes tipos de estaca / Scion production of mint in function of age and different types of cutting

O objetivo deste trabalho foi verificar a influência de diferentes idades e tipos de estacas na produção de mudas de Mentha arvensis L. Foram avaliados quatro diferentes tipos de estacas: estacas apicais retiradas da parte aérea; estacas retiradas da parte aérea mediana; estacas retiradas da ponteira do estolão e estacas retiradas da parte mediana dos estolões. Os diferentes tipos de estaca foram avaliadas com 25 e 40 dias de cultivo. Foram empregadas estacas com 4-5cm de comprimento, enterradas 2/3 no substrato comercial Plantmax®. Foram avaliados, nos dois períodos de enraizamento (25 e 40 dias) nos diferentes tipos de estaca na bandeja, a altura, o número de brotações, a biomassa seca da parte aérea e das raízes e a porcentagem de mortalidade. As estacas, após os períodos de enraizamento foram transplantadas para canteiro e vasos. Foi também determinada a porcentagem de mortalidade e a biomassa seca da parte aérea, das raízes e estolões, após um cultivo por 30 dias. Os resultados mostraram que as estacas apicais da parte aérea aos 25 dias e aos 40 dias após o plantio na bandeja apresentaram maior enraizamento e desenvolvimento da parte aérea e baixa porcentagem de mortalidade. Aos 25 dias, as estacas apicais da parte aérea se desenvolveram estando as mudas aptas ao transplantio para o campo. Aos 30 dias após o transplantio, as estacas apicais da parte aérea apresentaram maior crescimento, exceto para biomassa seca dos estolões.

The objective of this research was to verify the influence of different age and types of cuttings in the production of scion of Mentha arvensis L. Four different types of cuttings were evaluated: apical and medium cuttings (from aerial part); tip and medium cuttings (from rhizome part). The different types of cuttings were evaluated at 25 and 40 days of age. The cuttings used had 4-5cm long and covered with Plantmax® substrate to a depth of 2/3. Two rooting periods (25 and 40 days) from the different types of cuttings were evaluated: height, shoots number, aerial and roots dry biomass and percentage of mortality. The cuttings after the rooting period were transplanted to bed and pots. The mortality and the aerial part, roots and rhizome dry biomass were determined 30 days after being cultivated. The results showed that apical cuttings from aerial part at 25 and 40 days after being planted in trays had higher rooting, aerial part development and low mortality. At 25 days the aerial part developed and the scion was ready to be transplanted to field. Thirty days after transplanted apical cuttings from aerial part showed a higher growth, except for rhizome dry biomass.