RÉSUMÉ
Background: Anemia is the most common micronutrient deficiency. Despite repeated efforts taken under the national program, high prevalence of anemia still observed across various life stages of females in India over the past two decades. Methods: This cross-sectional study aimed to discern the prevalence of anemia in the non-pregnant and non-lactating females of Ambala, Haryana, between the ages of 6 months to 49 years using the gold standard cyan-met-hemoglobin method. Results: A total of 1, 21, 202 study subjects, with a mean age of 28.9±19.3 years were tested for hemoglobin across 524 villages, out of which 97,305 (80.1%) females were found to be anemic with mean hemoglobin level of 10.8±1.4 gm% with 53.2% of all females being moderately anemic. Conclusions: This study reported that anemia is extremely prevalent in all walks of life of females. Multipronged strategy is to be followed to curb anemia from various age groups of females.
RÉSUMÉ
Title: Effects of sodium dodecyl sulphate on enhancement of lipoxygenase activity of hemoglobin Authors: Ezebuo, F C Eze, S O O Chilaka, F C Keywords: Hemoglobin Linoleic acid Lipoxygenase Met-hemoglobin Oxodienes Sodium dodecyl sulphate Issue Date: Dec-2012 Publisher: NISCAIR-CSIR, India Abstract: Lipoxygenases comprise a family of non-heme iron-containing enzymes that catalyze the stereospecific dioxygenation of polyunsaturated fatty acids with 1, 4-cis-cis-pentadiene structure. Hemoglobin, a heme iron-containing protein has been reported to have lipoxygenase activity but the assay conditions that could enhance the activity remain obscure. Therefore, establishment of optimum assay conditions for lipoxygenase activity of hemoglobin could allow modeling of hemoglobin as lipoxygenase. Hemoglobin was extracted from blood of an identified individual of genotype AA. The hemoglobin was dialyzed at 4 oC for 24 h against 50 mM Tris-HCl buffers (pH 8.5 and 7.2) and effects of sodium dodecyl sulphate (SDS) and linoleic studied at pH 5.0 and 7.2 with UV–VIS Titration Spectrophotometry. The results show that 3.3, 8.6 and 88.1% concentrations of met-hemoglobin were found in presence of 0.0 mM SDS at pH 5.0 and 7.2, 1.043 mM SDS at pH 7.2 and 0.404 mM SDS at pH 5.0 respectively. Also, the difference spectra of hemoglobin in presence of linoleic acid showed positive peak at 285 nm which suggest the presence of oxodienes–a reaction product of hydroperoxidase activity of lipoxygenase. Formation of met-hemoglobin/met-myoglobin is highly correlated with lipid oxidation. Since highest concentration of met-hemoglobin (88.1%) was observed in presence of 0.404 mM SDS at pH 5.0, lipoxygenase activity of hemoglobin was enhanced in presence of SDS under these conditions.