Effect of Fluoxetine on Metabolic Diversity of Intestinal Microbiota in Rats With Depression / 胃肠病学

Background: Studies have shown that intestinal microbiota is closely related to the occurrence and development of depression, however, the regulatory mechanism of the classic antidepressant fluoxetine on intestinal microbiota is still unclear. Aims: To investigate the mechanism of fluoxetine in regulating intestinal microbiota structure and metabolic diversity in rats with depression. Methods: Thirty SD rats were randomly divided into blank group, model group and fluoxetine group. Depression rat model was established by chronic unpredictable mild stress (CUMS) combined with solitary care. Fluoxetine hydrochloride dispersible tablets 3.17 mg•kg

Exploring Biodiversity for Prevention and Control of Cancer and other Non-Communicable Diseases using Indigenous Environmental Resources: An Interdisciplinary Research

Background: An interdisciplinary research of public health, biomedical and pharmaceutical sciences is neededfor integrating qualitative and quantitative researches undertaken. It hence requires focus on public beneficencefor non-communicable diseases. Purpose: To study anticancer activities of soil samples of Central India andits stability for applied public health use. Material and Methods: Screening on Actinomycetes isolates obtainedfrom rural and urban farm soils illustrating arginase production was conducted from equated soil samples ofgeo-representative localities and adjoining areas of Bhopal, India. Enrichment Technique (CDSEA) was usedfor detection of extracellular production of L-arginase and their anticancer activities using MTT 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay along with characterization and probioticproperties of selected isolate. Results: L-Arginase activity quantified by ornithine (21.06-117.92 U/mg) wasfound in isolates BRD-21, KAR-73, BHA-162, BAR-199, ARH-210, HAB-228. Urea release (15.88 – 59.79 U/mg protein) depicted L- arginase activity in crude enzyme samples. It shows noticeable anticancer activity.Morphological and biochemical characterization of these isolates revealed metabolic diversity. Isolate KAR 73produced collagenase (specific activity 57.8 U/mg), L-asparaginase (specific activity 116 U/mg) and L-arginasewith tolerance to higher temperature (45°C) and salt concentration (2-8% w/v). Equal concentrations ofcrude L- arginase from these isolates inhibited growth and proliferation of colorectal adenocarcinoma celllines (19.99%-38.65%) under in-vitro conditions. Conclusion: Arginine depletion through arginase activity isevidenced for potential effectiveness in cancer treatment especially adenocarcinomas and squamous cellcarcinoma. It is useful for wider public health purposes