Preparation of molecular imprinted polymers for solid-phase extraction of L-tetrahydropalmatine / 中草药

Objective: To prepare the molecularly imprinted polymer (MIPs) of L-tetrahydropalmatine (L-THP) by the molecular imprinting technique and study on solid-phase extraction. Methods: Using L-THP as template, methyl acrylic acid (MAA) as functional monmer, and ehtylene glycol dimethacrylate (EDMA) as a cross-linking agent to prepare the L-THP-MIPs. A test was conducted to investigate the selectivity and the specificity of solid-phase extraction. Results: The experiment showed that the MIPs had the specific adsorption to L-THP, but did not have the specific adsorption to corydaline the structural analogue with L-THP. Conclusion: The L-THP-MIPs have a good selectivity and the specificity of L-THP.

Application of computational simulation and spectral analysis in preparation of Tanshinone IIA imprinted polymer / 中草药

To prepare Tanshinone IIA (TSIIA) molecularly imprinted polymers (MIPs) with affinity and high selectivity to TSIIA and study its mechanism. Taking TSIIA as the template molecular, synthesizing the MIPs at the surface of silica nanoparticles by grafting copolymerization in the toluene solution. A molecular modeling approach was used to elucidate template-monomer interaction. The interaction between template molecule and functional monomer was studied by UV spectrometry. The theoretical molar ratio of template-monomer was 1∶3 and methyl acrylic acid (MAA) was a better functional monomer than acrylamide (AAM) for MIPs synthesis. The MIPs can be used for the extraction and separation of TSIIA.

Determination of Related Substances in Methylacrylic Acid-Ethyl Acrylate Copolymer by HPLC / 中国药房

OBJECTIVE:To establish a method for the determination of related substances in methylacrylic acid- ethyl acrylate copolymer. METHODS:HPLC was performed on the column of Agilent C18 with mobile phase of methanol-phosphate buffer(70∶30,V/V) at the flow rate of 1.0 ml/min,the detection wavelength was 202 nm,column temperature was 20 ℃,and the sample size was 20 μl. RESULTS:The linear range was 0.001%-0.015% for both methylacrylic acid(r=0.999 4)and ethyl acrylate(r=0.999 1);RSDs of precision,stability and reproducibility tests were lower than 2%;the average recoveries of methylacrylic acid and ethyl acrylate were respectively 86.59%(RSD=2.3%,n=9)and 91.24%(RSD=3.5%,n=9). CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of related substances in methyl acrylic acid-eth-yl acrylate copolymer.