LIPASE-CATALYZED ENANTIOSELECTIVE AMMONOLYSIS OF (?)-?- METHYLBENZYL ACETATE IN MICROAQUEOUS PHASE / 微生物学通报

Novozym 435 was selected from four lipases and two proteinase because of its high catalytic activity and enantiosectivity.For the ammonolysis of (?)-?-methylbenzyl acetate,The effect of ammonia sources,the concentration of enzyme and substrates on the reaction were further explored .under the optimum conditions of this study,after 6h reaction,with the enantiomeric excess of the remaining (-)-?-methylbenzyl acetate was found to be higher than 99%.