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@#<b>Objective</b> To investigate the effects of low-dose nuclear radiation exposure on the body by analyzing the antioxidant indices, immune indices, lymphocyte proliferation activity, and blood biochemical indices of persons exposed to long-term low-dose nuclear radiation, and to provide a basis for radiation protection and occupational health monitoring. <b>Methods</b> Eighty nuclear radiation workers were selected as the exposure group, and another 30 non-exposure personnel were selected as the control group. In both groups, blood biochemistry, serum total antioxidant capacity (T-AOC), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), lymphocyte proliferation activity, proliferating cell nuclear antigen (PCNA), apoptosis factors Bcl-2 and Bax, lymphocyte transformation rate, and lymphocyte micronucleus rate were measured. <b>Results</b> Compared with the control group, T-AOC, GSH-Px, SOD, cell proliferation activity, PCNA, Bcl-2, lymphocyte transformation rate, white blood cell count, and platelet count in the exposure group were significantly decreased, while MDA and Bax were significantly increased (<i>P</i> < 0.05). The lymphocyte micronucleus rate showed no significant difference between the two groups (<i>P</i> > 0.05). <b>Conclusion</b> Long-term low-dose exposure to nuclear radiation has certain effects on related indices of workers, but does not cause significant damage. The personnel exposed to nuclear radiation should enhance the awareness of protection and strengthen scientific protection to reduce radiation damage.
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Objective To investigate the micronucleus rate of peripheral blood lymphocytes in radiation workers exposed to chronic low dose ionizing radiation in Jiangsu province to adopt corresponding protective measures. Methods From January to December 2019, 504 in-service radiation workers were taken as the radiation group, 105 healthy adults who were to be engaged in radiation work by pre-job occupational health examination were served as the control group. We compared the micronucleus rate of peripheral blood lymphocytes measured by conventional culture. Results The micronucleus rate in the radiation group was significantly higher than that in the control group (P < 0.001) and the micronucleus rate in the female radiation workers was higher than that in the male (P < 0.01); The difference of micronucleus rate in different age groups was statistically significant (P < 0.05), among which aged 41～50 years being higher than that of those aged 21～30 years. Besides, The difference of micronucleus rate between different working age groups was statistically significant (P < 0.05), with higher rates in the 21～30 year range than in the 0～10 year range. (P < 0.05). Differential micronucleus rates among different types of work were statistically significant (P < 0.01), with the highest rate of micronucleus among staff in radiotherapy positions compared to other positions. What’s more, the highest rate of micronucleus among radiology staff in units of different levels was found in municipal hospitals, with statistically significant differences in micronucleus rates between provincial hospitals, enterprises (P < 0.05) and municipal hospitals (P < 0.01). Conclusion The micronucleus rate of radiation workers was significantly higher than that of non-contact personnel, and the micronucleus rate of female radiation workers is higher than that of men. Moreover, the highest rate was found among radiation workers. Therefore, radiation protection and daily management of this population should be strengthened.
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Objective To explore the advantages of automatic chromosome harvester in lymphocyte micronucleus test. Methods The peripheral blood of 80 radiation workers was collected, then lymphocytes were harvested with an automatic chromosome harvester and manual methods. After Giemsa stained the slides, the micronucleus of the lymphocytes was analyzed under a microscope. Results For lymphocytes from the same batch of specimens, the time of the automatic harvester (185 min) was less than that of the artificial group (350 min). The micronucleus rate (1.04‰ ± 0.63‰), micronucleus cell rate (0.89‰ ± 0.69) and micronucleus positive rate (70.0%) that obtained by the harvester were all higher than manual method (0.60‰ ± 0.68‰, 0.51‰ ± 0.55‰, 48.8%), and the difference was statistically significant (P < 0.01). Conclusion The automatic chromosome harvester can shorten the experimental operation time, reduce the workload of laboratory personnel, and protect the physical and mental health of laboratory personnel. At the same time, it can increase the detection rate of lymphocyte micronucleus, which can provide more accurate detection data for the clinic.
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Objective@#To perform lymphocyte micronucleus analysis on radiation workers with long-term exposure to low doses ionizing radiation, Evaluate the health condition of radiation workers, and provide the evidence for strengthening surveillance of radiation workers.@*Methods@#From January 1, 2013 to December 21, 2016, a statistical analysis and evaluation was conducted of the peripheral lymphocytes micronucleus rate in 5 901 radiation workers who had undergone medical examinations of employees at Chinese Academy of Medical Sciences Institute of Radiation Medicine.@*Results@#The micronucleus rates in radiation workers of the on-job group were higher than the pre-job group (P<0.01) . Significant difference was found among the different sex (t=5.97) , different types (χ2=378.69) , different levels of work units (χ2=115.48) . Significant difference was found among the micronucleus rates of 672 radiation workers of the on-job group from 2013 to 2016 (χ2=92.57, P<0.01) .@*Conclusion@#The peripheral lymphocytes micronucleus rate of radiation workers were significantly higher than non-contact workers. Significant increasing trend of micronucleus rates was noted among the radiation worker with increasing exposure time. The peripheral lymphocytes micronucleus rates of interventional therapy workers were highest. The peripheral lymphocytes micronucleus rates of Private hospitals workers were highest. This phenomenon deserves attention. Protection needs to be strengthened to ensure the health of radiation workers.
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OBJECTIVE: To analyze chromosome damage and its possible influencing factors in patients with occupational chronic benzene poisoning. METHODS: Fifty patients with occupational chronic benzene poisoning were selected as chronic benzene poisoning group,and 53 workers without occupational exposure to benzene and other toxic substances were chosen as control group by using convenience sampling method. Questionnaire and routine blood test were conducted on all study subjects. Micronucleus rate test was performed by micronucleus blocking cytokinesis assay. RESULTS: Peripheral blood tests of chronic benzene poisoning group showed significantly reduced hemoglobin level,counts of red blood cells,white blood cells,platelets,lymphocytes and neutrophils( P < 0. 01),and higher lymphocyte micronucleus rates compared to control group( !: 6. 26‰ vs 3. 91‰,P < 0. 01). The proportion of increased lymphocyte micronucleus rate in chromic benzene poisoning group was also higher than that in control group( 46. 0% vs 5. 7%,P < 0. 01). The multivariate Poisson analysis results indicated that the time after disengagement from benzene exposure was the influencing factor of micronucleus rate in chronic benzene poisoning group( P < 0. 05),after adjusting the confounding factors of gender,age,smoking status,alcohol drinking status and working age of benzene exposure. CONCLUSION: Occupational chronic benzene poisoning leads to increase of chromosome damage in lymphocytes of patients. The time after disengagement from benzene exposure was positively correlated with chromosome damage.
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Objective: To explore the prophylactic and curative effects of Shengxue Pills on formaldehyde-induced cell damage of lung, liver, spleen, bone marrow, and peripheral blood lymphocytes in mice and the mechanisms. Methods: The mice were randomly divided into five groups: control, Shengxue Pill prophylaxis, Shengxue Pill therapy, and two model groups. ELISA was used to detect hydroxyl radicals, superoxide anion radical, and ornithine decarboxylase (ODC) activities in the lung, liver, and spleen cells. Bone marrow cells and peripheral blood lymphocyte micronucleus rate were observed with high power lens. Results: Hydroxyl radicals, superoxide anion radicals, and ODS activities in the cells of the organs in mice were investigated, and the number of bone marrow cells and peripheral blood lymphocyte micronucleus rate in mice in the prophylacitc and therapeutic groups were significantly greater than those in the model groups, respectively as well as in the normal group. Conclusion: Shengxue Pills could prevent and cure the formaldehyde-induced cell damages of the lung, liver, spleen, bone marrow, and peripheral blood lymphocytes of mice.
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Objective: To study the prevention and rehabilitation of Shengxue Pills on liver, spleen, bone marrow cells, peripheral blood lymphocyte of mice injured by low-dose radiation and their mechanisms. Methods: Mice were randomly divided into five groups: control, Shengxue Pills prevention, Shengxue Pills rehabilitation, model 1 (corresponding to rehabilitation), and model 2 (corresponding to prevention) groups. Using the ELISA method to detect the activity of hydroxyl radical, superoxide anion radical, and ornithine decarboxylase (ODC) in liver and spleen cells. The bone marrow cells and peripheral lymphocyte micronucleus rate were observed under high power lens. Results: Compared with control group, the amounts of hydroxyl radical and superoxide anion radical in the two model groups were obviously increased. Compared with the corresponding model groups, the above indexes in prevention group and rehabilitation group were significantly decreased. Compared with the control group, the activity of ODC in mice of the two model groups was obviously enhanced, but bone marrow cells and peripheral lymphocyte micronucleus rate were increased. Compared with the corresponding model groups, the activity of ODC in mice of prevention and rehabilitation groups was weakened, and bone marrow cells and peripheral lymphocyte micronucleus rate were decreased. But each index of spleen cells showed no significant difference. Conclusion: Shengxue Pills could effectively prevent and rehabilitate the damage in liver cells, peripheral blood lymphocytes, and bone marrow cells of mice injured by low-dose radiation.
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@# Objective To observe the effect of “Shengbai Tablets” on increasing the numbers of leucocyte, enhancing lymphocyte proliferation and reducing the micronucleus rate of cells.Methods The leukopenia model of mouse was established with cyclophosphamide, and the changes of the leukocyte count, lymphocyte proliferation and micronucleus rate before and after Shengbai Tablets treatment were observed.Results After treatment, the leukocyte count, lymphocyte proliferation and micronucleus rate of the Shengbai Tablets group were ( 7.470 ±1.852)×109/L, (14768.23±8316.86) and (6.02±1.72)‰ respectively, all were significantly better than that of the group without Shengbai Tablets treatment ( P<0.05～0.01).Conclusion Shengbai Tablets is able to increase the numbers of leucocyte, enhance the lymphocyte proliferation and reduce the micronucleus rate.