Phytochemical and antimicrobial screening of crude methanolic leaf extract of Peucedanum winkleri H. Wolff.

Phytochemical investigation of the leaves of Peucedanum winkleri H. Wolff, revealed the presence of secondary metabolites. The extract from total extraction with methanol was screened for its antimicrobial activity against Staphylococcus aureus, Methicillin resistant Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pyogenes, Proteus mirabilis, Candida albicans and Candida krusei using agar-well difussion method. The result indicated that the extract inhibited the growth of one or more test pathogens and were compareable with those of the standard drugs used. The minimun inhibitory concentration (MIC) ranges from 5-10 mg/ml and the minimun bactericidal/fungicidal concentration (MBC/MFC) ranges from 20-40 mg/ml. The result of the study shows justification for the use of the plant for the treatment of infectious diseases caused by these bacteria and fungi pathogens. It was concluded that P. winkleri H. Wolff could be a potential source of active antimicrobial agents and a detailed assessment of antimicrobial activity of the plant material in other solvents extract, isolation and characterization of active compounds from the most active extract is on-going.

Resistance of Pseudomonas aeruginosa Against Five Antiseptics / 中华医院感染学杂志

OBJECTIVE To explore the resistance of Pseudomonas aeruginosa isolated in clinic against five antiseptics involving in povidone iodine(Iodophor),glutaraldehyde,chlorhexidine,symclosene(trichloroisocyanurate) and benzalkonium bromide.METHODS The susceptibility test of P.aeruginosa was determined by PhoenixTM-100 system.Minimun inhibitory concentration(MIC) of povidone iodine,glutaraldehyde,chlorhexidine,symclosene and benzalkonium bromide was detected by liquid dilution method.RESULTS The resistant rates of ampicillin/sulbactam,chloramphenicol,tetracycline and trimethoprim-sulfamethoxazole in 190 isolates of P.aeruginosa were all more than 98.0%.However,P.aeruginosa was to imipenem and meropenem were 15.3% and 6.8%.It was found that P.aeruginosa possessed the most resistant to glutaraldehyde and symclosene with its MIC50 being 32 ?g/ml and 64 ?g/ml.But the MIC50 of chlorhexidine and benzalkonium bromide were only 1 ?g/ml and 2.4 ?g/ml.Meanwhile,time-kill assays indicated that chlorhexidine could still produce rapid and powerful bactericidal effects at a concentration of 1MIC after 10 min treatment.CONCLUSIONS There are distinct differences in P.aeruginosa against povidone iodine,glutaraldehyde,chlorhexidine,symclosene and benzalkonium bromide.It is very important that antiseptics should be used rationally.Measurements should be taken to decrease dissemination of resistant bacteria and prevent nosocomial infection.