Résumé
Background: Studies have indicated that there are two main pathogenetic pathways of colorectal cancer, chromosomal instability pathway and microsatellite instability (MSI) pathway. Aims: To investigate the expressions and clinical significance of mismatch repair protein (MMRP) and p53 protein in colorectal cancer. Methods: Immunohistochemical SP staining was used to detect the expressions of 4 MMRP and p53 protein in 276 colorectal cancer patients from Jan. 2013 to Dec. 2017 at Jiading Central Hospital of Shanghai, and their relationships with clinicopathological features were analyzed. The influence of MSI on survival rate of patients with colorectal cancer was analyzed. Results: The deficit of expression rate of MMRP was 13.0%. MSI was associated with histological type, TNM staging, Schistosomiasis infection (P0.05). The positivity expression rate of p53 was 44.9%, and the expression was correlated with histological type, TNM staging, lymph node metastasis, Schistosomiasis infection (P0.05). MSI was negatively correlated with p53 expression (r=-0.169,P<0.05). The 1-, 3-, 5-year survival rates in MSI group and MSS group were 100%, 97.2%, 83.1% and 96.7%, 81.9%, 38.9%, respectively, and the difference was statistically significant (χ2=12.582, P=0.001). Conclusions: MSI and p53 are closely related to the clinicopathological features of colorectal cancer, and have some values in predicting the degree of malignancy and prognosis of colorectal cancer. MSI is negatively correlated with p53 expression, which indicates that the two may participate in different stages of development and progress of colorectal cancer.
Résumé
Background: Colorectal cancer (CRC) is a commonly seen cancer, and is a heterogeneous disease entity with a diverse biological pathogenesis. Aims: To investigate the expressions of mismatch repair protein (MMRP) and Ki-67 in CRC, and analyze the correlations of microsatellite instability (MSI), Ki-67 with clinicopathological features of CRC. Methods: Clinicopathological data of 90 CRC patients from Jan. 2014 to Dec. 2016 at the First Affiliated Hospital of Soochow University were retrospectively analyzed. Immunohistochemical staining was used to detect the protein expressions of 4 MMRP (MLH1, PMS2, MSH2 and MSH6) and Ki-67 in CRC patients. Correlations of MSI, Ki-67 with clinicopathological features of CRC patients were analyzed. Correlation of MSI with Ki-67 was also analyzed. Results: The loss expression rate of MMRP was 16.7%, and that of MLH1, PMS2, MSH2 and MSH6 were 11.1%, 11.1%, 6.7% and 4.4%, respectively. Positivity rate of Ki-67 was 90.0%. MSI was correlated with tumor location (P0.05). Expression of MLH1 was positively correlated with expression of PMS2 (r=0.577, P<0.05), and expression of MSH2 was positively correlated with expression of MSH6 (r=0.739, P<0.05). Conclusions: The loss expressions of MLH1, PMS2 are more common than those of MSH2, MSH6 in CRC. MSI is correlated with tumor location and Ki-67 is correlated with tumor location and gross type; they may be of some significance for the diagnosis and prediction of prognosis of CRC. However, MSI is not correlated with Ki-67, and joint detection of MMRP and Ki-67 could not improve the diagnostic accuracy of CRC.