Antipyretic and anti-inflammatory effects of Mosla chinensis based on integration processing technology of origin / 中草药

Objective To compare the effect of integration processing technology of origin with traditional cutting processing technology of Mosla chinensis on pyretic rats caused by LPS and inflammatory RAW264.7 cells induced by LPS, and provide the feasible basis for the implementation of integration processing technology of origin and traditional efficacy of the relieving exterior effects of M. chinensis. Methods The rats were intervened by M. chinensis (volatile oil, decoction), and the model of heat syndrome was established by ip of LPS. The temperature changes of the fever rats were observed. The levels of serum prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), adenosine cyclic phosphate (cAMP), interleukin-1β (IL-1β), and myeloperoxidase (MPO) in liver tissue were detected. The effects of integration processing of origin and traditional cutting processing of M. chinensis on heat syndrome rats were compared. The cell viability of LPS stimulated RAW264.7 macrophages cells after administration of different concentration of M. chinensis (decoction and volatile oil) was detected, and the NO secretion was determinated by ELISA for comparing the anti-inflammatory effect of two processing methods. Results The treatment of processed M. chinensis can decrease the anus temperature and the content of PGE2, TNF-α, CAMP, IL-1β, MPO, and enhance cellular immunity. Moreover the efficacy of integration processing of origin is better than traditional cutting processing, and the efficacy of M. chinensis decoction is better than volatile oil. Conclusion The integration processing technology of origin for M. chinensis is feasible.

EFFECTS OF THE VOLATILE OIL OF MOSLA CHINENSIS MAXIM ON IMMUNE RESPONSES / 中国免疫学杂志

The effects of the volatile oil ofMosla Chinensis Maxim(MCMVO) onimmune responses in mice were stu-died.When mice immunized with SRBC were fed with MCMVO (190mg/kg/d),the specific antibody levelin serum and the activity of antibo-dy forming cells increased.on nons-pecific immunity,MCMVO also acti-viated alternative complement path-way and increased the activity oflysozyme in serum.MCMVO alsoenhanced peripheral ANAE~+ lympho-cytes ratio in comparison with cont-rol.MCMVO could also significantlyincreased the spleen weightof young mice.The resultsmentioned above suggestedthat MCMVO could raise thenonspecific and specific immunity.It might be expected that MCMVOcould be useful in precaution andtreatment of infectious diseases.