Résumé
Abstract: We report range extensions for three species of Amazonian erethizontids, Coendou bicolor, C. ichillus, and C. nycthemera. We record C. ichillus for the first time in Brazil, from Rio Japurá, state of Amazonas. We record C. bicolor for the first time in the state of Amazonas, which represents a range extension of approximately 905 km. We also extend the occurrence of C. nycthemera 620 km to the south into Mato Grosso state. All records are based on museum specimens, highlighting the importance of scientific collections as biodiversity databases and emphasizing the lack of research on Amazonian porcupines.
Resumo: Aqui nós relatamos ampliação de distribuição de três espécies de eretizontídeos amazônicos: Coendou bicolor, C. ichillus e C. nycthemera. Nós registramos pela primeira vez C. ichillus no Brasil, no Rio Japurá, estado do Amazonas. Registramos C. bicolor pela primeira vez no estado Amazonas, o que representa uma ampliação de distribuição de aproximadamente 905 km. Também estendemos a ocorrência de C. nycthemera 620 km ao sul, no estado de Mato Grosso. Todos os registros são baseados em espécimes de museu, enfatizando a importância das coleções científicas como bancos de dados da biodiversidade e a destacando ausência de pesquisas para porcos-espinhos amazônicos.
Résumé
Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64 percent of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the Emílio Goeldi Museum (Museu Paraense Emílio Goeldi, MPEG), Belém, Pará, Brazil; and 3 samples from the Museum of Vertebrate Zoology (MVZ) University of California, Berkeley, USA. The specimens sampled ranged in age from 18 to 108 years old. Our methodology allowed the recovery of up to 700 bp of mitochondrial DNA and 400 bp of nuclear genes. Thereafter, it is useful for genetic diversity studies of three-toed sloths and could be applied to other animals.