Proteomic Analysis of the Serum in Patients with Acute Coronary Syndrome

BACKGROUND AND OBJECTIVES: Proteomics is a new technology that allows the detection and identification of several proteins at a given time in a sample. There are currently few reports concerned with the proteomic study of serum from patients during acute coronary syndrome. We performed proteomics to analyze the modifications in the serum protein map of patients with acute coronary syndrome (ACS). SUBJECTS AND METHODS: We investigated the serum from 12 patients who suffered with acute myocardial infarction (AMI), 12 patients with unstable angina (UA) and 13 age- and sex-matched patients as the control group. Two-dimensional electrophoresis, Coumassie staining and image analysis were performed. Mass spectrometry was performed to identify the selected spots. RESULTS: For the two-dimensional electrophoresis with using a pH range of 3 to 10, two different areas within the serum protein map were observed, and this showed differences between the groups. In area 1, three fibrinogen gamma chain isoforms were identified. All of them were increased in the serum from the AMI and UA patients when compared with the control group. In area 2, four fibrinogen beta chain isoforms were identified. Three isoforms of them were increased in the serum from the AMI and UA patients. CONSLUSION: Three fibrinogen gamma chain isoforms were identified and they were increased in the serum from ACS patients. Four fibrinogen beta chain isoforms were identified and three isoforms of them were increased in the serum from ACS patients.

The kinetic alterations of plasma prekallikrein and antithrombin Ⅲ in rats following isoproterenol-induced myocardial infarction / 中国病理生理杂志

The kinetic alterations of plasma prekallikrcin(PKA) and antithrombin Ⅲ (AT-Ⅲ) following myocardial infarction induced by 2 injections subcutaneously of 85 mg isoproterenol/kg at 24 hours interval in rats were detected in this study, and the relationship between PKA, AT-Ⅲ and myocardial ischemia induced by isoproterenol were also discussed. It was found that the activity of PKA decreased significantly and AT-Ⅲ tended to decrease at 4 hours after the first injection of isoproterenol, both of the PKA and AT-Ⅲ at 24 hours were lower than those at 4 hours and evidently lower than those of normal control, and at 48 hours after the first injection of isoproterneol the PKA was still maintained at the same level of 24 hours but the AT-Ⅲ almost returned to the normal level.