RÉSUMÉ
Objective To investigate the mechanism of inhibiting the Notch signaling pathway in promoting neural regeneration after neural stem cells (NSCs) transplantation in rats with cerebral ischemia. Methods The model of focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO) method . NSCs were cultured in vitro and transplanted into the striatum ischemic area. In the experiment, 40 SD rats were divided into sham operation group, model group, transplantation group (transplanted neural stem cells), and N-[N-(3, 5-difluorohenacetyl )-L-alanyl ]-S-phenylglycinet-butyl ester( DAPT) + transplantation group. The degree of neuronal damage in each group was observed by HE staining. The expressions of Notchl, Hesl and Hes5 in the brain tissue of each group were detected by immunohistochemistry and Western blotting. Results Compared with the sham operation group, the neuron injury in the model group was severe, and nuclear pyknosis and nuclear lysis were observed. The Notchl, Hesl and Hes5 positive cells increased significantly, and the expression of Notchl, Hesl and Hes5 proteins were significantly up-regulated (P<0. 05). Compared with the model group, the neuronal damage in the transplantation group and the DAPT+ transplantation group were all relieved, and the Notchl, Hesl and Hes5 positive cells were partially expressed, and the expression of each protein decreased (P<0. 05). The DAPT+ transplantation group neurons were compared. The damage was obviously restored, and the expression of protein-positive cells and protein further decreased (P<0. 05). Conclusion Inhibition of Notch signaling pathway can promote nerve regeneration after neural stem cell transplantation in rats with cerebral ischemia. The mechanism is mainly related to down-regulation of Notch 1 , Hesl and Hes5 expression.
RÉSUMÉ
To explore the protective effect of nerve function of Buyang Huanwu Decoction on cerebral ischemia/reperfusion rats after the transplantation of neural stem cells (NSCs) . Methods Thread bolt method was used to establish middle cerebral artery occlusion model. Drug groups were given Buyang Huanwu Decoction (14. 8 g kg"1 d " 1) by gavage after the rats being sober. NSCs were transplanted to rat brain after making the model 24 hours later. Zea Longa neurobehavioral behavioral score was used to observe neural function defect, and TTC staining to detect the volume of cerebral infarction, and Nissl staining to detect Nissl body integrated optical density (IOD), and Immunohistochemical staining to detect expression of Bcl-2 and Bax. Results Compared with sham operation group, the nerve function defect appeared, and the volume of cerebral infarction increased significantly, the integral optical density of Nissl body was reduced and the ratio of Bcl-2 to Bax was reduced in model group (P < 0. 05) . Compared with model group, the nerve function defect was reduced, the volume of cerebral infarction was reduced, the integral optical density of Nissl body increased, and the ratio of Bcl-2 to Bax increased in BYHWD group, Transplant group and BYHWD + Transplant group (P < 0. 05). Compared with transplant group, the nerve function defect was reduced, the volume of cerebral infarction was reduced , the integrated optical density of Nissl body increased , and the ratio of Bcl-2 to Bax increased in BYHWD + Transplant group (P < 0. 05). Conclusions Buyang Huanwu Decoction can enhance the neuroprotective effect after NSCs transplantation in cerebral ischemia/reperfusion rats.