Supercritical transesterification of vegetable oil and ethanol: temperature and residence time effects on phase formation and water content

Supercritical transesterification has emerged as a readily available alternative for biodiesel production since no catalyst is required, thereby generating fewer waste products. In this research, the supercritical transesterification of refined vegetable oil and aqueous ethanol was carried out at temperatures 400 to 480 °C and a 12:1 ethanol to oil molar ratio, to assess the effect of temperature and residence time in the formation of a homogeneous phase, effluent appearance and increased water content derived from glycerol etherification. The results showed that water was produced at temperatures higher than 400 °C, as expected from the occurrence of glycerol etherification, and that prolonged times resulted in gas and soot formation, indicating esters decomposition. Through water mass balances, it was possible to identify the set of operation conditions in which the water formed from glycerol etherification matched with the maximum expected according to the proposed reaction scheme.

La transesterificación supercrítica se ha propuesto como una alternativa para la producción de biodiesel ya que no requiere catalizador de esta manera se generan menos residuos. En esta investigación, la transesterificación supercrítica de aceite vegetal refinado y etanol acuoso se llevó a cabo a temperaturas en el rango 400 a 480 °C y relación molar etanol a aceite de 12:1, para evaluar el efecto de la temperatura y el tiempo de residencia en la formación de una fase homogénea, apariencia del efluente e incremento del contenido de agua resultado de las reacciones de eterificación del glicerol. Los resultados mostraron que se produjo agua a temperaturas mayores a 400°C, atribuida a la eterificación del glicerol, y que tiempos de residencia prolongados resultaron en formación de gas y hollín, indicativo de reacciones de descomposición de esteres. A través de balances de masa, fue posible identificar el conjunto de condiciones de operación a las cuales el agua formada por la eterificación del glicerol coincide con el valor máximo esperado de acuerdo con el esquema de reacción propuesto.

A transesterificação supercrítica foi proposta como uma alternativa para a produção de biodiesel porque não requer catalisador e, dessa forma, gera menos resíduos. Nesta pesquisa, a transesterificação supercrítica de aceite vegetal refinado e etanol acuoso foi conduzida a temperaturas entre 400 e 480 °C e uma relação molar de etanol e aceite de 12: 1, para avaliar o efeito da temperatura e do tempo de residência na formação de uma fase homogênea, apariência do efluente e aumento do conteúdo de água resultante das reações de eterificação do glicerol. Os resultados mostraram que se produziu água a temperaturas maiores que 400°C, atribuída à eterificação do glicerol, e que os tempos de residência prolongados resultaram na formação de gás e hollín, indicativo de reações de decomposição de ésteres. Por meio de balanças de massa, foi possível identificar o conjunto de condições de operação em que a água formada pela eterificação do glicerol coincide com o valor máximo esperado de acordo com o esquema de reação proposto.

Study on the correlation between telomere dysfunction of human gastric mucosa and chronic atrophic gastritis / 中华消化杂志

Objective:To investigate the correlation between telomere dysfunction of human gastric mucosa and chronic atrophic gastritis (CAG).Methods:From February 12, 2019 to July 10, 2020, at Endoscopy Center, Guang′anmen Hospital, China Academy of Chinese Sciences, 30 patients received endoscopy and pathological diagnosed with CAG (CAG group) were collected, and 30 patients with chronic non-atrophic gastritis (CNAG) were collected at the same time (CNAG group). The relative telomere length was detected by real time fluorescent quantitative polymerase chain reaction. The expression of telomere repeat binding factor (TRF) 1, TRF2 and protection of telomere (POT) 1 at protein level were detected by immunohistochemical staining and semi-quantitative analysis. Spearman analysis was used to analyze the correlation between the relative telomere length of gastric mucosa and the protein expression levels of TRF1, TRF2 and POT1. Mann-Whitney U test and independent sample t test were used for statistical analysis. Results:The relative telomere length of the gastric mucosa in the CAG group was shorter than that in the CNAG group (0.67 (0.51 to 1.17) vs. 1.06(0.69 to 1.37)), and the difference was statistically significant ( U=297.00, P=0.024). The protein expression levels of TRF1, TRF2, and POT1 in the CAG group were all higher than those in the CNAG group, respectively (4.26±2.49 vs. 1.86±1.34, 10.12±2.76 vs. 8.78±2.81, 4.22±2.48 vs. 2.53±1.62), and the differences were statistically significant ( t=8.05, 3.23, 5.39; P<0.001, =0.001, and <0.001). In the CAG group, the protein expression levels of TRF2 and POT1 in gastric mucosa were negatively correlated with the relative telomere length ( r=-0.477 and -0.417, P=0.008 and 0.022). Conclusions:The telomere dysfunction is related to the pathogenesis of CAG. The change of telomere binding protein expression level is involved in the shortening of telomere and pathological process of CAG patients.

Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Vibrio parahaemolyticus is a major pathogen frequently found in seafood. Rapid and accurate detection of this pathogen is important for the control of bacterial foodborne diseases and to ensure food safety. In this study, we established a one-pot system that combines uracil-DNA glycosylase (UDG), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12b (Cas12b) for detecting V. parahaemolyticus in seafood. This detection system can effectively perform identification using a single tube and avoid the risk of carry-over contamination.

Evaluation of water stress and co-inoculation of Azospirillum brasilense and Rhizobium tropici in beans (Phaseolus vulgaris l) / Avaliação do estratégio de água e co-inoculação de Azospirillum brasilense e Rhizobium tropici in beans (Phaseolus vulgaris l) / Evaluación del estrés hídrico y la coinoculación de Azospirillum brasilense y Rhizobium tropici en frijoles (Phaseolus vulgaris l)

Climate change has caused major changes in abiotic factors, with water stress as the greatest threat to agricultural production. The measures aimed at alleviating the problems caused by this limiting production factor have occurred through the adoption of sustainable strategies, especially microbial biotechnology, which uses the interactions between the microorganism and the plant, ensuring productive quality and inducing plant resistance to stresses biotic and abiotic. The objective of the present work was to evaluate the biological nitrogen fixation and the development of bean seedlings, with co-inoculation of two types of inoculants, which were subjected to water stress by different pot capacities. The experiment was conducted in a greenhouse, at Universidade Paranaense - UNIPAR, from April to June 2019. The experimental design was completely randomized (DIC), with 5 replications, 16 treatments and 80 experimental units. The cultivar used was SCS Riqueza. The parameters evaluated were pot capacity (25%, 50%, 75% and 90%); small, large and total nodules, shoot and root length, dry and fresh weight, total carbon and nitrogen. The evaluation of the morphological parameters of the bean seedlings indicated that the co- inoculation technique promoted beneficial effects for the dry mass parameters of shoot, nodule and root. The analysis of the percentage of carbon and nitrogen in the tissues of the seedlings provided an increase in the concentration of these elements in treatments that involved co-inoculation (Azospirillum brasilensis and Rhizobium tropici) with pot capacities of 25 and 75% (CV), demonstrating that the association of microorganisms is beneficial in the limiting water situation.(AU)

A mudança climática tem causado grandes mudanças nos fatores abióticos, sendo o estresse hídrico a maior ameaça à produção agrícola. As medidas destinadas a aliviar os problemas causados por este fator limitante de produção ocorreram através da adoção de estratégias sustentáveis, especialmente a biotecnologia microbiana, que utiliza as interações entre o microorganismo e a planta, garantindo a qualidade produtiva e induzindo a resistência da planta ao estresse biótico e abiótico. O objetivo do presente trabalho foi avaliar a fixação biológica de nitrogênio e o desenvolvimento de mudas de feijão, com co-inoculação de dois tipos de inoculantes, que foram submetidos ao estresse hídrico por diferentes capacidades de vaso. A experiência foi realizada em uma estufa, na Universidade Paranaense - UNIPAR, de abril a junho de 2019. O projeto experimental foi completamente randomizado (DIC), com 5 réplicas, 16 tratamentos e 80 unidades experimentais. A cultivar utilizada foi a SCS Riqueza. Os parâmetros avaliados foram a capacidade do vaso (25%, 50%, 75% e 90%); nódulos pequenos, grandes e totais, comprimento do rebento e da raiz, peso seco e fresco, carbono total e nitrogênio. A avaliação dos parâmetros morfológicos das mudas de feijão indicou que a técnica de co-inoculação promoveu efeitos benéficos para os parâmetros de massa seca do turião, nódulo e raiz. A análise da porcentagem de carbono e nitrogênio nos tecidos das mudas proporcionou um aumento na concentração destes elementos nos tratamentos que envolveram a co-inoculação (Azospirillum brasilensis e Rhizobium tropici) com capacidades de vaso de 25 e 75% (CV), demonstrando que a associação de microorganismos é benéfica na situação limite da água.(AU)

El cambio climático ha provocado importantes cambios en los factores abióticos, siendo el estrés hídrico la mayor amenaza para la producción agrícola. Las medidas encaminadas a paliar los problemas causados por este factor limitante de la producción se han producido mediante la adopción de estrategias sostenibles, especialmente la biotecnología microbiana, que utiliza las interacciones entre el microorganismo y la planta, asegurando la calidad productiva e induciendo la resistencia de la planta a los estreses bióticos y abióticos. El objetivo del presente trabajo fue evaluar la fijación biológica de nitrógeno y el desarrollo de plántulas de frijol, con la co-inoculación de dos tipos de inoculantes, que fueron sometidos a estrés hídrico por diferentes capacidades de maceta. El experimento se realizó en un invernadero, en la Universidade Paranaense - UNIPAR, de abril a junio de 2019. El diseño experimental fue completamente al azar (DIC), con 5 repeticiones, 16 tratamientos y 80 unidades experimentales. El cultivar utilizado fue SCS Riqueza. Los parámetros evaluados fueron capacidad de maceta (25%, 50%, 75% y 90%); nódulos pequeños, grandes y totales, longitud de brotes y raíces, peso seco y fresco, carbono y nitrógeno total. La evaluación de los parámetros morfológicos de las plántulas de frijol indicó que la técnica de coinoculación promovió efectos beneficiosos para los parámetros de masa seca de brotes, nódulos y raíces. El análisis del porcentaje de carbono y nitrógeno en los tejidos de las plántulas proporcionó un aumento en la concentración de estos elementos en los tratamientos que involucraron la coinoculación (Azospirillum brasilensis y Rhizobium tropici) con capacidades de maceta de 25 y 75% (CV), demostrando que la asociación de microorganismos es beneficiosa en la situación de agua limitante.(AU)

Long-chain alkanol-alkyl carboxylic acid-based low-viscosity hydrophobic deep eutectic solvents for one-pot extraction of anthraquinones from Rhei Radix et Rhizoma / 药物分析学报

Natural long-chain alkanol and alkyl carboxylic acid were used to prepare novel hydrophobic deep eutectic solvents(HDESs).These HDESs are liquid at room temperature and have low viscosity(＜12.26 mPa-s),low polarity(lower than that of methanol,ChCl-based deep eutectic solvents and other reported HDESs),and low density(＜0.928 g/mL).A simple one-pot method based on a novel HDES-water two-phase extraction system was constructed for the extraction of weak-polarity bioactive components,anthraquinones,from Rhei Radix et Rhizoma.This HDES-based new extraction method does not consume hazardous organic solvents and can obtain a total anthraquinone yield of 21.52 mg/g,which is close to that obtained by the Chinese pharmacopoeia method(21.22 mg/g)and considerably higher than those by other reported HDESs-based extraction methods(14.20-20.09 mg/g,p＜0.01).The high extraction yield can be mainly attributed to the severe destruction of the RRR cell walls by the extraction system and the excellent dissolving ability of novel HDESs for anthraquinones.

Treatment of scapulohumeral periarthritis by Fuyang-pot warming combined with electroacupuncture stimulation / 针刺研究

OBJECTIVE: To observe the clinical therapeutic effect of Fuyang-pot warming combined with electroacupuncture (EA) in the treatment of scapulohumeral periarthritis (SPA). METHODS: A total of 90 cases of SPA patients were randomized into EA, Fuyang-pot warming and EA plus Fuyang-pot warming (combination) groups (n=30 per group). Fuyang-pot warming including pressing, mild moxibustion, scraping-pushing, cupping, tapping, etc. was applied to Fengchi (GB20), Dazhui (GV14), Jianjing (GB21), Jianyu (LI15), Zhongfu (LU1), Ashi-point, etc., and EA (2 Hz /100 Hz,1-1.5 mA) was appled to GB20, GV14, GB21, LI15, Binao (LI14), Tiaokou (ST38), Chengshan (BL57), Ashi-point, etc. The treatment was performed for 30 min every time, once every other day for 2 weeks. The visual analogue scale (VAS, 0-10 points) was used to assess the pain severity. The Constant-Murley shoulder assessment scale (100 points in total, including 15 points in pain severity and 20 points in daily living activities, 40 points in joint motion range, and 25 points in myodynamia) was used to assess the functional state of the shoulder. The rating scale of the American Shoulder and Elbow Surgeons (ASES, 4 grades) was used to evaluate the ability of daily living activities. RESULTS: Following the treatment, intra-group comparison showed that the VAS score was significantly reduced in the three groups in comparison with their own pre-treatment (P0.05). CONCLUSION: Fuyang-pot warming combined with EA is effective in relieving pain, and enhancing the daily life quality in scapulohumeral periarthritis patients.

Mechanism of lung injury of rats induced by inhalation of white smoke from burning smoke pot / 中华烧伤杂志

Objective@#To explore mechanism of lung injury of rats induced by inhalation of white smoke from burning smoke pot.@*Methods@#Forty-eight Sprague Dawley rats were divided into control group (n=12) and injury group (n=36) according to the random number table. Rats in injury group were placed in smoke-induced injury experimental equipment fulled with white smoke from burning smoke pot for 5 minutes to make lung injury, and rats in control group were placed in smoke-induced injury experimental equipment fulled with air for 5 minutes to make sham injury. Six rats in injury group at post injury hour (PIH) 6, 24, and 72 and six rats in control group at PIH 72 were collected to observe pathological changes of lung tissue and pathological score of rats in the two groups by hematoxylin-eosin staining, to detect expression of nuclear factor-κB (NF-κB) p65 mRNA in lung tissue of rats by reverse transcriptional polymerase chain reaction, and to detect content of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-6 in lung tissue of rats by enzyme-linked immunosorbent assay. Data were processed with one-way analysis of variance and t test.@*Results@#At PIH 72, lung tissue structure of rats in control group was clear and complete, with no inflammatory cell infiltration. At PIH 6, there was edema, hemorrhage, and inflammatory cell infiltration in lung tissue of rats in injury group. At PIH 24, edema, hemorrhage, and inflammatory cell infiltration in lung tissue of rats in injury group aggravated. At PIH 72, area of edema in lung tissue of rats in injury group was enlarged, with obvious hemorrhage and inflammatory cell infiltration. At PIH 6, 24, and 72, pathological score of lung tissue of rats in injury group was (3.43±0.86), (5.39±0.93), and (9.99±0.84) points, respectively, obviously higher than that of rats in control group at PIH 72 [(2.11±0.20) points, t=3.659, 8.450, 22.355, P<0.05]. As time post injury prolonged, pathological scores of lung tissue of rats in injury group were significantly increased (F=121.244, P<0.01). At PIH 6, 24, and 72, expression of NF-κB p65 mRNA in lung tissue of rats in injury group was 15.5±4.3, 25.9±1.8, 30.9±3.5 respectively, significantly higher than that of rats in control group at PIH 72 (7.8±0.8, t=4.315, 20.445, 14.408, P<0.01). As time post injury prolonged, expression of NF-κB p65 mRNA in lung tissue of rats in injury group gradually increased (F=32.691, P<0.01). At PIH 6, 24, and 72, content of TNF-α, IL-1β, and IL-6 in lung tissue of rats in injury group was significantly higher than that of rats in control group at PIH 72, respectively (t=7.650, 8.968, 6.827, 6.726, 8.978, 3.460, 5.420, 13.289, 16.438, P<0.01). At PIH 24, content of TNF-α and IL-1β in lung tissue of rats in injury group was higher than that of rats in the same group at PIH 6 and 72, respectively (t=3.409, -2.549, 4.047, -4.100, P<0.05). At PIH 24 and 72, content of IL-6 in lung tissue of rats in injury group was respectively higher than that of rats in the same group at PIH 6 (t=8.273, 9.711, P<0.05).@*Conclusions@#After inhaling white smoke from burning smoke pot, rats are inflicted with lung injury by increasing expression of NF-κB p65 mRNA and content of TNF-α, IL-1β, and IL-6, and induce pathological changes of edema, hemorrhage, and inflammatory cell infiltration of lung tissue.

Improved synthesis of analgesic pentazocine / 中国药科大学学报

@#A new synthetic process of pentazocine has been developed. Starting from 3, 4-dimethylpyridin and benzyl chloride, via quaternarization, Grignard reaction addition/reduction/salt, cyclization, hydrogenation, alkylation and recrystallization, pentazocine were obtained in a total yield of 8. 4%(with a molar mass of 3, 4-dimethylpyridin), with purity greater than 99. 97%. The improved synthetic route is suitable for industrial production.

Clinical observation on the treatment of chronic pelvic inflammatory disease with pelvic acid capsule combined with antibiotics / 中国生化药物杂志

Objective To investigate the clinical effect of potting net capsule combined with antibiotics in the treatment of chronic pelvic inflammatory disease.MethodsThe clinical data of 156 patients with chronic pelvic inflammatory disease admitted from October 2013 to October 2016 were retrospectively analyzed.The patients were randomly divided into control group (n=77) and observation group (n=79).Routine antibiotic therapy and Pelvic acid net capsule combined with antibiotic therapy.The changes of TCM syndromes and treatment efficiency and the level of serum inflammatory factors were observed, and the incidence of adverse reactions was observed and compared.ResultsAfter treatment, the scores of TCM syndromes were evaluated and compared between the two groups.There were significant differences between the two groups.The scores of the control group were significantly higher than those in the observation group (P<0.05).The treatment group was 93.67%, which was significantly higher than that of the control group (72.73%), and the difference was statistically significant (P<0.05).There were no significant differences in CRP, TNF-α, IL-6 and IL-1β levels between the two groups before and after treatment.After different treatment, the two groups were compared (P<0.05), and there was significant difference between the two groups after treatment.There were statistically significant differences between the two groups after treatment.The observation group was significantly lower than the control group (P<0.05).There were no adverse reactions in both groups.ConclusionThe treatment of chronic pelvic inflammatory disease with conventional antibiotic therapy combined with pelvic inflammatory capsule can improve the clinical symptoms, reduce the inflammatory level and improve the therapeutic effect.It is a safe and effective treatment program.

The design and clinical application of a new type of disposable heparin free dialysis tube / 中国实用护理杂志

Objective To investigate the clinical effect of a new type of disposable heparin freedialysis tube in uremic patients without heparin dialysis. Methods A total of 110 patients withoutheparin dialysis were divided into control group and observation group by random digits table method with55 cases each. Based on the same pre dialysis and dialysis for heparin saline flushing of discontinuoussaline on the control group using the traditional dialysis pipe for hemodialysis without heparin, theobservation group used disposable non heparin dialysis pipe for hemodialysis without heparin. The numberof patients with venous catheter blockage, blood loss and completion of dialysis time in two groups wasrecorded. Results The number of patients with venous catheter blockage, blood loss and completion ofdialysis time was 6, 6, 47 cases in control group and 0, 0, 54 cases in observation group. The differences were statistically significant (χ2=4.407, 4.407, 4.356, P<0.05). Conclusions The new disposableheparin free dialysis tube can reduce the loss of blood and prolong the treatment time, and the operation issimple, safe and effective, and has good application value and application prospect.

Clinical observation on the treatment of kidney deficiency syndrome of polycystic ovary syndrome by meal nourishing decoction combined with letrozole / 实用医学杂志

Objective To investigate the medicated side warm nest pot combined with letrozole in the treatment of multiple bursal ovarian syndrome (PCOS) superiority of the clinical treatment of kidney deficiency syndrome in ovulation dysfunction infertility,provide the basis for clinical treatment. Methods 78 cases of infertile patients(randomly selected from January 2014 to June 2015 hospitalized in Hainan Medical University Hospital) due to kidney deficiency syndrome in patientswith ovulatory dysfunction resulting from PCOS. 39 cases in the control group received oral letrozole only. 39 cases in experimental group took oral warm nest pot combined with letrozole,continuous treatment for four menstruation cycle. Adverse reactions,ovulation rate,pregnancy rate and safety index of the two groups before and after treatment of menstruation were observed and recorded. Results The effective rate of the control group was 74.36%,and the effective rate of the experimental group was 87.18%. The difference was statistically significant(P < 0.05). The ovulation rate of the control group was 47.71%,which was lower than that of the experimental group (79.07%),and the difference was statistically significant (P < 0.05). The pregnancy rate in the control group was 12.82%,while that in the experimental group was 33.33%,and the difference was statistically significant (P < 0.05). Patient′s irregular menstruation improved in the two groups , while the experimental group did better than the control group. Conclusion Effect of warm nest pot combined with letrozole on kidney deficiency syndrome of ovulation dysfunction in PCOS is remarkable ,with little side effects.

Investigation and analysis of hemodialysis blood tubing vein pot particle formation and related factors / 中国实用护理杂志

Objective To investigate the composition of particulate matter in hemodialysis and to explore the influencing factors of particulate matter formation. Methods The composition of the particles was determined by chemical experiments and electron microscopy.Fifty-four outpatients in hemodialysis centers were divided into pellets and non-particulate groups(34 and 20 cases).Thirty-four variables were collected by literature review and their influencing factors were analyzed. Results The particles were white thrombus,formed by aggregated platelets and fibrin aggregates,and embedded in a small amount of red blood cells. Logistic regression analysis showed that low molecular weight heparin was statistically significant (OR = 1.001, P <0.01). In addition, male, smoking, dry weight, hypersensitive C-reactive protein, venous pressure, dialysate temperature, blood vessel thrombosis and granule formation were statistically significant(P<0.05). Conclusions To improve the degree of attention to white thrombosis, the injection of low molecular weight heparin sodium into the machine before the vascular access intravenous injection, for the analysis of the Results to take appropriate care measures to reduce the production of white thrombosis is important,and can be white thrombosis.The repeated formation is seen as a sign of blood vessel thrombosis.

Theoretical fragmentation: origins and repercussions in Work and Organizational Psychology / Fragmentação teórica: suas origens e desdobramentos na Psicologia Organizacional e do Trabalho / La fragmentación teórica: sus orígenes y desarrollos en Psicología Organizacional y del Trabajo

The fragmentation problem has been a concern in WOP for jeopardizing the construction of good theories and the establishment of a solid theoretical field. In this article, we describe the theoretical fragmentation in WOP and map the main types and origins. We discuss the main conceptual problems currently observed in WOP. Then we discuss the role of political and ideological issues and structural factors of scientific development within the fragmentation process. If features of WOP, like splits and internal divisions, favor the emergence of fragmentation, we should also consider broader factors that permeate different fields of humanities and applied social sciences and reverberate on WOP in the same manner. Finally, we seek to differentiate two mainstreams: the fragmentation that weakens the field; and the multiplicity of approaches and concepts that constitute the scientific process. We reflect on the challenge of differentiating them, and on steps to reduce fragmentation.

Os problemas de fragmentação vêm sendo uma preocupação na POT por ameaçar a construção de boas teorias e a constituição de um campo teórico sólido. Neste artigo, descrevemos a fragmentação teórica em POT e buscamos mapear seus principais tipos e origens. Discutimos o que é um conceito e os principais problemas conceituais atualmente observados na POT. Em seguida, debatemos o papel das questões políticas e ideológicas e dos fatores estruturais do desenvolvimento científico no processo de fragmentação. Se de um lado a própria constituição da POT favorece a emergência de fragmentações, por outro devemos considerar fatores mais amplos, que atravessam diferentes campos e reverberam do mesmo modo na POT. Buscamos, por fim, diferenciar dois quadros: as fragmentações que fragilizam o campo; e a multiplicidade de abordagens e conceitos que compõem o fazer científico. Refletimos sobre o desafio de diferenciá-los, e sobre etapas necessárias para a redução da fragmentação.

El problema de la fragmentación ha sido una preocupación para la POT por amenazar a la construcción de las buenas teorías y el establecimiento de un campo teórico sólido. En este artículo, se describe la fragmentación teórica en POT y se busca mapear sus principales tipos y orígenes. Discutimos respecto de lo que es un concepto y los principales problemas conceptuales que se observan actualmente en el POT. A continuación, debatimos acerca del papel de las cuestiones políticas e ideológicas y de los factores estructurales del desarrollo científico en el proceso de fragmentación. Si, por un lado, la constitución de la POT favorece la aparición de fragmentación, por otro, hay que tener en cuenta factores que abarcan diferentes campos, que repercuten del mismo modo en la POT. Finalmente, se pretende diferenciar dos cuadros: las fragmentaciones que debilitan el campo; y la multiplicidad de enfoques y conceptos que componen el trabajo científico. Se reflexiona sobre el reto para diferenciarlos, y las etapas necesarias para reducir la fragmentación.

Origin, consolidation, and perspectives of Work and Organizational Psychology / Origem, consolidação e perspectivas da Psicologia das Organizações e do Trabalho / Origen, consolidación y perspectivas de la Psicología de las Organizaciones y del Trabajo

This essay is a reflection on the trajectory of Work and Organizational Psychology (WOP). The content analyzes its growth and introduction into society. Along this path, WOP took on the phenomena and problems of the relationships between individual, work, and society. Today, WOP presents a set of scientific, technical, and cultural productions integrating the conceptual and instrumental patrimony of society. WOP has enriched the understanding of performance at work by applying the concepts of Psychology to explain the relation between behavior and subjectivity. Facing the re-institutionalization of work from a form of employment to one of precarious autonomous work, WOP will be challenged to understand new phenomena that will arise within its territory. Its solid experience and firmness in the search for efficacy and for existential conditions sustains the assurance with which it faces the evolution of economic production. WOP has become a specialization capable of responding to the re-institutionalization of work now in progress in society.

Este ensaio consiste numa reflexão sobre a origem e trajetória da Psicologia das Organizações e do Trabalho (POT). Seu conteúdo analisa sua construção e inserção na sociedade. Nessa trajetória, a POT assumiu os fenômenos e problemas das relações entre a pessoa, o trabalho e a sociedade e ganhou confiança dos gestores e da sociedade. Hoje, a POT apresenta produção científica, técnica e cultural no patrimônio conceitual e instrumental da sociedade. A POT enriqueceu a compreensão do desempenho no trabalho aplicando conceitos da Psicologia na interface comportamentos e subjetividade. Diante da institucionalização do trabalho da forma dos empregos para o trabalho autônomo precário, a POT será desafiada pela compreensão de novos fenômenos. A solidez de sua experiência e sua firmeza na busca da eficácia e das condições existenciais alimentam sua segurança frente à evolução da produção econômica. A POT tornou-se capacitada para enfrentar a atual reinstitucionalização do trabalho, na sociedade.

Este ensayo es un análisis de la trayectoria de la Psicología de las Organizaciones y del Trabajo (POT). Su contenido expone su origen e inserción en la sociedad asumiendo los fenómenos y problemas entre la persona, el trabajo y la sociedad. Hoy, la producción conceptual, técnica y cultural de la POT integra el patrimonio instrumental de la sociedad. La POT enriqueció la comprensión de la relación entre el desempeño y la subjetividad. Delante de la reinstitucionalización del trabajo como empleo para actividad autónoma precaria, nuevos fenómenos y problemas surgirán para los cuales la POT está capacitada y motivada. Su experiencia y firmeza en búsqueda de la efectividad del desempeño y de las condiciones de existencia la capacitan para enfrentar eses nuevos fenómenos y problemas en la evolución de la producción económica.

Synthesis, biological activity and molecular docking research of N-{(4-oxo-thiochroman-3-yl)phenyl-methyl}acetamide derivatives as α-glucosidase inhibitors / 药学学报

In order to develop potent antidiabetic agents that have inhibitory effect to α-glucosidase, twelve β-acetamido ketone derivatives such as N-{[(substituted-4-oxo-thiochroman-3-yl)phenyl]-methyl}acetamide are designed and synthesized through one-pot Dakin-West reaction. Their chemical structures are confirmed by 1H NMR, 13C NMR, IR and HR-MS. In vitro α-glucosidase inhibition assays of compounds 4a-4l were carried out using glucose oxidase method. The result indicated that most of them possess inhibitory activity in vitro. Compound 4k showed the most potent inhibitory activity with 87.3% inhibition of α-glucosidase at the concentration of 5.39 mmol·L-1. The structure-activity relationship of these β-acetamido ketone derivatives was discussed preliminarily. Moreover, the molecular docking method was used to study the interaction mode of compound 4k and α-glucosidase. Our results will be helpful for designing of α-glucosidase inhibitors in the future.

Cultivo de mudas de eucalipto irrigadas com esgoto doméstico tratado / Cultivation of eucalyptus seedlings irrigated with treated domestic sewage

Avaliou-se o cultivo de mudas de eucalipto da espécie urograndisirrigadas com esgoto doméstico tratado em estação com reator anaeróbio e lagoa de polimento. A irrigação das mudas, em vaso e com base na capacidade de pote (CP) para o solo, foi efetuada em experimento com seis tratamentos (T), com quatro repetições cada. Em T1, T2, T3 e T4, a irrigação com esgoto foi com lâmina a 100, 80, 60 e 40% do índice CP, respectivamente; em T5 e T6, a irrigação foi com água a 80% de CP. Apenas T5 teve adição de fertilizantes (NPK). Uma boa eficiência da estação de tratamento foi obtida durante os 3 meses de experimento, com remoção de demanda bioquímica de oxigênio, demanda química de oxigênio e helmintos de 90, 87 e 100%, respectivamente; portanto, com qualidade de efluente adequada para irrigação de arbóreas. Os desempenhos de T2 e T3 foram os melhores em termos de parâmetros de crescimento da planta (altura, diâmetro do caule, diâmetro da copa, número de folhas e matéria seca); comparativamente, em T2, acumulou-se mais nitrogênio nas folhas e caules. Os resultados obtidos mostram que o cultivo de mudas de eucalipto irrigadas com esgoto tratado, aproveitando os nutrientes e com lâminas adequadas (entre 80 e 60% do índice CP), é viável agronomicamente.

The cultivation of Eucalyptus urograndis seedlings irrigated with domestic sewage effluent of a treatment plant with anaerobic reactor and polishing pond was evaluated. Seedling pots were irrigated based on the soil pot capacity (PC) in six treatments (T) with four repetitions each. The pots in T1, T2, T3 and T4 were irrigated with slide heights at 100, 80, 60 and 40% of the PC index, respectively; in T5 and T6, irrigation was only with water and slide heights at 80% of PC. Only T5 received additional nutrients (NPK). Along the experiment (3 months), the treatment plant showed good removal efficiencies of biochemical oxygen demand (90%), chemical oxygen demand (87%) and helminth eggs (100%); therefore, with adequate effluent quality for tree irrigation. T2 and T3 showed the best results in terms of plant development parameters, like height, stalk diameter, cup diameter, number of leaves and dry matter; comparatively, T2 showed the highest nitrogen accumulation in the leaves and stalks. The results show that cultivation of eucalyptus seedlings irrigated with treated sewage, taking advantage of the nutrients and with adequate slide heights (between 80 and 60% of PC index), is agronomically viable.

Empresas juniores de psicologia: capacitar, desenvolver e transformar / Junior enterprises in psychology: training, developing, and transforming

Ao longo dessa apresentação procurei demonstrar que o espaço de formação proporcionado pelas EJ faz sentido político, educacional e pedagógico para a Psicologia, de modo geral, e para a sub área de POT, de forma específica. Espero ter deixado claro que a natureza da resistência que elas enfrentam decorre de uma visão particular sobre nossa profissão e sobre a própria função da universidade. Argumentei ainda, que as EJs se apresentam como uma atividade extracurricular que permite a complementação da formação para os estudantes que dela se aproximam e que seus benefícios não são exclusivos. Outros tipos de atividades formativas podem oferecer resultados assemelhados. Entretanto, seus benefícios justificam a sua existência e funcionamento. Por fim, apresentei as principais formas que vêm sendo utilizadas para resolver a questão da relação entre as EJs e as universidades em função de sua natureza jurídica...

Throughout this presentation I have tried to show that the training space provided by the JEs makes political, educational, and pedagogical sense for Psychology, in general, and for the work and organizational psychology sub area, specifically. I hope I have made it clear that the nature of the resistance they face stems from a particular view of our profession and about the role of the university itself. I have also argued that the JEs are presented as an extracurricular activity that offers complementary training for students who participate in this activity, and that its benefits are not exclusive. Other types of training activities can offer similar results. However, its benefits justify its existence and operation. Finally, I have presented the main ways being used to address the issue of the relationship between JEs and universities due to its legal nature...

One-pot synthesis of graphene-chitosan nanocomposite modified carbon paste electrode for selective determination of dopamine

Background A simple, rapid, low-cost and environmentally friendly method was developed to determine dopamine (DA) in the presence of ascorbic (AA) and uric acid (UA) based on a novel technique to prepare a graphene-chitosan (GR-CS) nanocomposite and modify it on the surface of carbon paste electrode (CPE). For our design, CS acts as a media to disperse and stabilize GR, and then GR plays a key role to selective and sensitive determination of DA. Results Under physiological conditions, the linear range for dopamine was determined from 1 × 10- 4 to 2 × 10- 7 mol/L with a good correlation coefficient of 0.9961 in the presence of 1000-fold interference of AA and UA. The detection limit was estimated to be 9.82 × 10- 8 mol/L (S/N = 3). In order to study the stability and reproducibility, GR/CS/CPE underwent successive measurements in 10 times and then tested once a d for 30 d. The result exhibited 98.25% and 91.62% activities compared with the original peak current after 10-time measurements and 30-d storage. Conclusion The GR/CS/CPE has wide linear concentration range, low detection limit, and good reproducibility and stability, which suggests that our investigations provide a promising alternative for clinic DA determination.

Servicios públicos domiciliarios: ¿derechos humanos o productos para la venta? Caso Medellín / Home utilities: are they human rights or products for sale? The Medellin Case

El presente artículo presenta los resultado de investigación encaminada a demostrar y visibilizar, en la ciudad de Medellín, las múltiples afectaciones a personas por la ausencia de servicios públicos domiciliarios en sus viviendas, aún y la capacidad técnica, operativa y financiera de la empresa prestadora de servicios en la ciudad: Empresa Públicas de Medellín (Epm E.S.P), lo que supone una inadecuada lectura de la realidad de la ciudad, principalmente en las zonas periféricas y zonas de alto riesgo. Esta situación lleva a que los ciudadanos recurran a las vías judiciales o a las vías de hecho para proveerse del servicio y en cualquiera de las dos vías se termina por generarle una serie de dificultades de orden jurídico, social y económico al Municipio que es, según la Ley 142 de 1994, el responsable de garantizar el derecho a los servicios públicos domiciliarios; situaciones que pudiesen contar con una solución más adecuada en tanto que ajustada a las realidad de la ciudad.

This current paper introduces the research results aiming at demonstrating and identifying, in the city of Medellin, the multiple effects to people by the lack of home utilities, still and the technical, operational, and financial capacities of the company providing services in the city: Public Companies of Medellin (EPM -ESP), which supposes an inadequate reading of the reality of the city, mainly on the outskirts and high-risk areas. This situation makes the citizens resort to the judicial routes or assault and battery in order for them to provide with the service, and in either of the two routes, a series of legal, social, and economic difficulties are derived, which becomes a problem to the Municipality, which according to the Law 142 as of 1994, is responsible for ensuring the people the right to home utilities, situations which could have a moreadequate solution to the extent that it can be adjusted to the reality of the city.

Novel One-Pot Three-Component Synthesis of New Ethyl 8-Methyl-3,6-Diphenyl-2,6-Dihydropyrimido[2,1-B][1,3,4]Thiadiazine-7-Carboxylate Derivatives and its Antimicrobial Activities.

A novel series of ethyl 8-methyl-3,6-diphenyl-2,6-dihydropyrimido[2,1-b][1,3,4]-thiadiazine-7- carboxylates 4 (a-m) were synthesized in good to excellent yield via cyclocondensation of 2-amino-5-pheny-6H- 1,3,4-thiadiazine 1 (a-d) with ethyl acetoacetate (2) and various substituted aldehydes 3 (a-d) in presence of ptoluene sulfonic acid (PTSA) in acetonitrile. The structures of these compounds 4 (a-m) were characterized by IR, 1H NMR, 13C NMR, Mass spectroscopic techniques and elemental analysis. All newly synthesized compounds 4 (a-m) were screened for antimicrobial activities.