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1.
Article de Chinois | WPRIM | ID: wpr-1021903

RÉSUMÉ

BACKGROUND:Three-dimensional(3D)printing is an emerging technology in the field of dentistry.It utilizes a layer-by-layer manufacturing technique to create scaffolds suitable for periodontal tissue engineering applications.Tissue scaffolds produced through 3D printing can possess controlled characteristics,including internal structure,porosity,and interconnectivity,making it an ideal strategy for periodontal tissue engineering. OBJECTIVE:To review the applications of 3D printed scaffolds in periodontal regeneration. METHODS:English search terms were"3D printing,periodontal tissue engineering,additive manufacturing,regenerative medicine,bioengineering,scaffold,bioprinting,periodontitis".Chinese search terms were"3D printing,additive manufacturing,periodontal tissue engineering,scaffolds,bio-inks,bioprinting,tissue engineering".Relevant literature published from 2000 to 2023 in PubMed and CNKI databases was retrieved and included in the review. RESULTS AND CONCLUSION:Over the past few decades,3D printing technology has made significant progress and breakthroughs in tissue engineering and biomedical fields.3D printing technology can provide highly personalized treatment programs,improve the suitability and therapeutic effect of therapeutic stents,and has broad application prospects in periodontal tissue engineering.In periodontal tissue engineering,3D printing applications can better mimic the complex structures of biological tissues and manufacture biocompatible scaffold materials with suitable mechanical and rheological properties.The layer-by-layer construction of tissue engineering scaffolds through 3D printing not only enables the creation of precise and intricate scaffold models for personalized treatment of periodontal disease but also facilitates the incorporation of complex microstructures and channels within the scaffolds to promote cell growth and tissue regeneration.

2.
Article de Chinois | WPRIM | ID: wpr-621449

RÉSUMÉ

[Objective] The biocompatibility between SGBG/PHBV and human PDLC were investigated to provide a basis for the choice of the scaff material of periodontal tissue engineering.[Methods] Human PDLC were cultured using tissue-explant technique.Seeding on 96 wells plate,9 wells for one group,Four different concentrations (100%,75%,50%,25%,0%) of maceration extract of SGBG/PHBV were added into the culture plantsafter 48-h cell seeding,the grades of the cytotoxicity of SGBG/PHBV was evaluated by MTT assay.Human PDLC cultured in vitro were collected and seeded on the three-dimensional scaffolds of SGBG/PH-BV,the cellular morphology and cell growth on the scaffolds were observed and photographed by scanning electronic microscope.HumanPDLC seeded on the three-dimensional scaffolds of SGBG/PHBV in the experimental group,and human PDLC seeded by DMEM in the control group,after 12-,24-,and 48-h cell seeding,got 27 simples for each group,and the affection of the SGBG/PHBV on cell secretory function was observed by spectrophotometry which assayed the biochemical indexes ALP in supinate.[Results] The grades of the cytotoxicity of SGBG/PHBV were 0 and 1.It was displayed that human PDLC adhered and proliferated well on the scaffold of SGBG/PHBV under the scanning electronic microscope.The significant difference of ALP in supinate between the experimental group and the control group (P < 0.05).[Conclusions] SGBG/PHBV had no cytotoxicity to human PDLC.SGBG/PHBV is potential to further study as the scaffolds of periodontal tissue engineering since it displayed the satisfactory biocompatibility with human PDLC.

3.
Article de Chinois | WPRIM | ID: wpr-595565

RÉSUMÉ

Objective:Because of the lower proliferation ability of periodontal ligament cells of elderly people,how to choose suitable scaffold materials for them becomes very important.The aim of this study was to observe the morphological manifestation of the periodontal ligament cells of elderly people seeded onto the three-dimensional porous nano-hydroxylapatite scaffolds.Methods: Periodontal ligament cells from elderly people were seeded onto the three-dimensional porous nano-hydroxylapatite scaffolds,cultured for 15 days in vitro,and observed for the morphological changes by scanning electron microscopy in comparison with those of young people.Results:Fifteen days after seeded on the three-dimensional porous nano-hydroxylapatite scaffolds,the periodontal ligament cells of the elderly people exhibited sufficient adhesive behavior,proliferated well and produced tiny calcium nodules,but the number of the nodules was smaller than that in the young people.Conclusion: The periodontal ligament cells of elderly people grow well on three-dimensional porous nano-hydroxylapatite scaffolds,and can be considered as a suitable scaffold for periodontal tissue engineering in elderly people.

4.
Article de Chinois | WPRIM | ID: wpr-542464

RÉSUMÉ

Objective:To evaluate the periodontal tissue regeneration effect of implantation of bone marrow stromal cells(BMSCs)-root-coralline hydroxyapatite(CHA) complex.Methods:BMSCs of beagle dogs were in vitro cultured on CHA scaffold and on tooth root surface respectively.Then BMSCs-root samples were inserted into BMSCs-CHA scaffolds to form BMSCs-CHA-root complexes.5 complexes were implanted on one side of mandible in each of the dogs 1 month after the 4th,5th and 6th teeth had been extracted.Root-CHA without BMSCs was implanted on another side as the control.All dogs were killed 16 weeks after implantation,and the periodontal tissue regeneration were observed histologically.Results:More new bone formation and more mature alveolar bone were observed in the test group than that in the control,and there was a clearly periodontal ligament-like tissue formation only in the test group.Conclusion:The complex of BMSC-root-CHA can improve the periodontal tissue regeneration.

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