RÉSUMÉ
Objective:To investigate the effects of unilateral ureteral obstruction on renal pelvic peristalsis and pacemaker cells in neonatal rats.Methods:An animal experimental study.Thirty-six 2-day-old newborn SD rats were randomly divided into the partial unilateral ureteral obstruction (PUUO) group, complete unilateral ureteral obstruction (CUUO) group, and sham operation group, with 12 rats in each group.One week after surgery, all rats were subjected to renal pelvic pressure (RPP) measurement by puncture.After measurement, the rats were euthanized, and their left renal pelvis and ureter were removed and fixed for histological examination.Parameters such as RPP, peristaltic wave frequency and amplitude at different perfusion speeds were recorded and compared, and the changes in pacemaker cells (atypical smooth muscle cells and Cajal-like interstitial cells) were also compared.The independent samples t-test was used for comparison between 2 groups, and the one-way ANOVA of variance was used for comparison among 3 groups. Results:In the sham operation group, the RPP increased gradually with the increase of perfusion speed; the frequency of peristaltic waves rose rapidly and then dropped after reaching the highest level with the increase of perfusion speed; similarly, the amplitude of peristaltic waves first increased and then decreased as the perfusion speed increased.In the PUUO group, the RPP increased rapidly with the increase of perfusion speed, higher than that in the sham operation group; the frequency of peristaltic waves was higher than that in the sham operation group, and it was relatively constant under the perfusion speed of 40 mL/h, but when the perfusion speed increased again, the frequency began to decline; the amplitude of peristaltic waves increased quickly and then declined at a faster rate than the sham operation group with the increase of perfusion speed.In the CUUO group, the basic RPP was 12 cmH 2O(1 cmH 2O=0.098 kPa); at the perfusion speed of 5 mL/h, the RPP rose gradually, and no plateau appeared; when the RPP reached 73 cmH 2O, the perfusate retrograded from the side of the puncture needle, then the RPP slightly decreased and then balanced, and no regular peristaltic waves were observed in the renal pelvis throughout the whole perfusion process.Immunofluorescence staining analysis showed the pacemaker cells were all located in the smooth muscle of the renal pelvic wall.The sham operation group had the highest positive rate, followed by the PUUO group and then the CUUO group. Conclusions:Ureteral obstruction has a significant impact on the peristalsis of the renal pelvis, and its impact on the peristaltic wave frequency and amplitude and RPP can be predicted.The reduction of pacemaker cells in the renal pelvis may be involved in the changes of renal pelvic peristalsis caused by ureteral obstruction, but further research is needed on how pacemaker cells regulate the peristalsis of the renal pelvis and ureter.
RÉSUMÉ
Objective To investigate the significance of changes of esophageal peristalsis in hiatal hernia patients. Methods 32 patients with symptom of gastroesophageal reflux(esophageal hernia 10, hiatal hernia with esophagitis 12 and esophagitis 10) and 9 patients without symptom of reflux were selected.Lower esophageal sphincter (LES) pressure ,esophageal peristaltic velocity ,amplitude and duration were detected respectively;the degree of esophagitis was identified with gastroscopy. Results LES pressures in hiatal hernia with esophagitis group and simple esophagitis group were significantly lower than those in control group , there was no significant difference between hiatal hernia group and control group . In hiatal hernia group ,the peristaltic amplitudes of proximal and distal esophagus (51.3?5.4 mmHg and 83.6?8.3 mmHg) were significantly higher than those in the other three groups , while esophagitis group was significantly the lowest . There were no significant difference about esophageal peristltic duration both of proxmial and distal esophagus between these four groups .Furthermore , distal esophageal peristaltic velocity was significantly higher in hiatal hernia group than that in the other three groups , and it was the lowest in esophagitis group .Conclusion The changes of esophageal peristalsis in patient with hiatal hernia play an important role in the pathogenesis of gastroesophageal reflux and mucosal injury.[