Résumé
Background: Garlic is naturally occurring sulphur containing dietary agent belong to Allium family. Members of this family i.e. Garlic, Onions are found to have beneficial effect on atherosclerosis and ischemic heart diseases in both experimental animals and in human beings. Objective: To compare the potency of garlic as antihyperlipidemic and antiplatelet and antithrombotic between its watery and alcoholic extract for their effects. Methods: 25 guinea pigs were fed cholesterol (0.5g/kg body weight) for an initial period of 4 weeks. Cholesterol was then discontinued and the animals were divided into 3 groups. Group-I (n=7) was fed with stock diet and was taken as control. Group-II (n=9) was given 1ml of watery extract and Group-III (n=9) was fed with 1ml of alcoholic extract of garlic with normal diet daily for 4 weeks. The garlic content of both the extract was 2 gm/ml by wt/vol. Fasting blood samples were collected at the end 4 weeks and finally at the end of the study i.e.8 weeks for estimation of serum cholesterol, serum triglyceride, LDC-C, HDL-C, VLDL-C,atherogenic index and platelet adhesiveness index. Bleeding time and clotting time were also detected in all the 3 groups.Results: In the present study, the watery and alcoholic extract of garlic shows significant hypolipidemic activity as they reduced significantly serum cholesterol, serum triglyceride, LDC-C, VLDL-C and atheriogenic index in hyperlipidemic guinea pigs (p<0.01) compared to control. The significant rise in HDL-C was observed in group II but not in group III animals. On comparison between two extracts, watery extract of garlic was more potent hypolipidemic agent, compared to alcoholic extract. Both extract significantly reduces platelet adhesiveness and bleeding time. But between two extract the watery extract was found more potent anti-thrombotic agent as it showed significant decrease in platelet adhesiveness (P<0.01). BT was significantly increased in group II compared to group I and III. Conclusion: It can be concluded that watery extract of Allium Sativum is more potent hypolipidemic and antiplatelet, and anti-thrombotic agent.
Résumé
Objective To investigate the adhesion molecule expression and functional status of platelets in lung cancer patients, and their relations with disease progression. Methods Using flow cytometry to measure the expression of surface antigens and functional status of platelets in 60 healthy control group, and 164 lung caneer patients. Results Comparing with control group, the expression in early group and mid term group(A group) of CD31, CD36, CD62, CD63 increased, and there is no significant meaning in the differences of surgery group (B group) of CD31, TSP, CD36, CD62, CD63. The expression in advanced group (C group) of CD31, TSP, CD36, CD62, CD63 increased. Comparing with A Group, the expression in B Group of CD36, CD62, CD63 decreased. The expression in C group of CD31, TSP, CD36, CD62, CD63 increased. Comparing with the small cell lung cancer, the expression of adenocareinorna of TSP, CD36,, CD62, CD63, squamous cell carcinoma of CD31, CD62, CD63, and alveolus cancer of CD31, TSP, CD63, all decreased. Conclusion The high level expression of platelet activation exists in patients with lung cancer of different stage, and decreased after operation. Platelet activation expressed significantly in the advanced stage of small cell lung cancer.
Résumé
Objective To investigate the effects of magnitude of surgical trauma and duration of operation on aggregation and adhesion of platelets during laparoscopy and conventional laparotomy cholecystectomy. Methods Thirty ASA Ⅰ-Ⅱpatients(male 11, female 19) undergoing elective cholecystectomy were studied . The mean age was(45 .2 ? 8.3) years and mean body weight(63.3 ?12. 6)kg. The patients were divided into two groups: laparoscopy group(group LC) and laparotomy group(group OC). Patients who took any drugs which may affect blood coagulation were excluded. Blood routine examination, coagulation and bleeding time were normal in all patients. In both groups anesthesia was induced with fentanyl 0. 1-0.2mg, propofol 2mg/kg and vecuronium 0.1mg/kg and maintained with isoflurane inhalation. Venous blood samples were taken after induction of anesthesia and 1h and 2h after operation was started. 10ml of blood was withdrawn from median cubital vein for the measurement of platelet adhesion(PAdT) and plasma level of platelet membrane glycoprotein(GPⅠ b/Ⅰ x) and von Willebrand factor(vWF) . Venepuncture was made at first attempt without using tourniquet. Plastic syringes were used and first 2ml of blood withdrawn was discarded . Glass ball method was used for measurement of PAdT which was calculated according to the following equation: Rate of platelet adhesion(% ) = /(No. of platelet before adhesion-No. of platelet after adhesion) No. of platelet before adhesionGP Ⅰ b/Ⅰ x and vWF levels were measured using ELISA method. Results There was no significant difference in PAdT and GP Ⅰ b/Ⅰ x and vWF levels after induction of anesthesia between two group. PadT level was significantly lower at 1 and 2h during surgery in group LC than that in group OC. GPⅠh/Ⅰx level was higher at 1h during operation in group OC than that in group LC and was much higher at 2h during surgery. vWF level decreased significantly at 2h during operation in both groups. Conclusions Platelet adhesion rate increases during operation and the severer the trauma the higher the platelet adhesion rate. GPⅠ b/Ⅰ x level is higher in group OC during operation than that in group LC probably due to severity of trauma. vWF is consumed during operation.