FINDING OF THE CHARACTERISTIC PARENTHESIS-LIKE STRUCTURE IN PNEUMOCYSTIS CARINII CYST STAINED BY TOLUIDINE BLUE O / 中国血吸虫病防治杂志

In this study, toluidine blue O stain was conducted in the lung smears of rats and mice with Pneumocystis carinii infection induced by corticosteroids. All of the cyst wall of Pneumocystis carinii was stained dark purple and was easily distinguished from the blue background. The parenthesis-like structure in the cyst could clearly be recognized in the lungs with both specimens just as with the Grocott's methenamine-silver nitrate method. It is considered that this structure can be a distinctive mark from fungi. The results of the experiment show that the toluidine blue O stain may become a practical technic for the diagnosis of Pneumocystis carinii pneumonia and can substitute the Grocott's method.