Résumé
Background: Reactive oxygen species (ROS) may cause extensive tissue damages in various disease conditions.It may also induce an irreversible structural and/or functional modification of proteins. Flavonoids and theirderivatives are the largest group in plant polyphenols that are known to have an antioxidant effect. The aim ofthe present study is to evaluate the antioxidant effects of red or white cabbage on bovine serum albumin (BSA).Methods: Fresh leaves of red or white cabbage were washed with distilled water, and sliced into small pieces.Finally, the pieces were dried and extracted with 80% ethanol overnight. The antioxidant activity of cabbageextracts were studied by ferric reducing antioxidant power (FRAP) and H2O2 scavenging assays. Statisticalanalysis: Statistical significances were analyzed by one-way analysis of variance (ANOVA) by using software Rversion 2.8.1 (R Development Core Team, 2008). Significant differences (p < 0.05) are denoted by differentletters. Results: Red and white cabbage extract showed a pronounced antioxidant activity. White cabbageexhibited a highest antioxidant activities compared to red cabbage extract. Conclusion: Both red and whitecabbages have a high antioxidant effects. White cabbage extract had higher antioxidant activity than red cabbageextract.
Résumé
Protein carbonyl content assay was conducted among eight groups of animals treated with lead, alcohol and vitamin E in individuals and in combination at two, four and eight weeks of duration. In two weeks of treatment, lead treated rats recorded 2.45 nmol/grams. In alcohol treated rats, it was recorded 2.60 nmol/gram of protein carbonyl content in liver tissue. Compared to control, it was recorded 2.01 nmol/gram. In vitamin E treated with lead and alcohol, protein carbonyl content was recorded 3.02 nmol/gram of tissue. In four weeks of duration, lead treated rats recorded 2.64 nmol/gram of tissue and in alcohol treated rats, protein carbonyl content was 2.87 nmol/gram and in lead with alcohol and vitamin E treated rats, protein carbonyl content was 3.29 nmol/gram. In eight weeks of treatment, maximum protein carbonyl content was recorded in lead with alcohol treated rats compared to control.
Résumé
Objective To study the protein oxidative damage and its possible mechanism caused by multi-walled nanotubes(MWCNTS)in mice.Methods Totally 20 Kun-ming mice were divided randomly into 4 testing groups(n=5 for each group),with 0.1,0.2 and 0.4mg/ml MWCNTS suspension injected groups and saline injected group as control group.After 5 days exposure,the protein carbonyl content was measured by using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage.Results The results showed that the protein carbonyl content in livers in 0.2 and 0.4mg/ml MWCNTS suspension injected groups were significantly higher than that in saline group(P