Résumé
Objective: To develop a new method for the simultaneous determination of two hydrophilic components and two lipophilic components of Radix et Rhizoma Salviae Miltiorrhizae. Methods: The HPLC-DAD method was employed using a column of Agilent Zorbax TC C18 (4.6 mm × 250 mm, 5 μm) with a mobile phase of methanol -2% acetic acid. The gradient elution program was as follow:0-15 min, 30% B-40% B; 15-20 min, 40% B-60% B; 20-25 min, 60% B-90% B; 25-40 min, 90% B. The detection wavelength was set at 281 nm and the temperature was 35°C. Results: The linearity was obtained over 3.76-120.20 μg · ml-1 (r=0.999 9) for rosmarinic acid, 34.20-109 4.5 μg · ml-1 (r=0.999 9) for salviamolic acid B, 0.64-20.32 μg · ml-1 (r=0.999 9) for clyptotanshinon, and 1.02-32.72 μg · ml-1 (r=0.999 6) for tanshinone II A. The RSDs of precision and stability of the sample were both less than 1% in 48 hours. The average recovery was between 99.72%-100.63%. Conclusion: The present method is simple and has satisfactory efficacy; it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.
Résumé
OBJECTIVE:To observe the effects of the compound preparation of Radix et Rhizoma Salviae Miltiorrhizae and Chuanxiongzine(DCs) on activity of superoxide dismutase(SOD),concentration of malondialdehyde(MDA) in erythrocytes and cerebral tissue in rats with Alzheimer's Disease(AD).METHODS:Alzheimer's Disease model was established in rats with aluminium trichloride and D-galactose,with Naofukang as positive control to observe the effects of compound preparation of DCs on the activity of SOD and the concentration of MDA in erythrocytes and cerebral tissue in rats.RE-SULTS:In DCs-treated group compared with Alzheimer's Disease model group,the activity of SOD increased significantly(P
Résumé
Objective To study the genetic diversity of ITS sequences of Radix et Rhizoma Salviae Miltiorrhizae (RRSM) and plants of Salvia L. and analyze the utility of ITS sequences in molecular authentication of RRSM and phylogenetic of plants of Salvia L. Methods The ITS gene fragment was amplified using a pair of primers. The PCR products were purified and sequenced by the methods of Sanger dideoxy. Results The DNA sequence of 222-224 bp ITS1, 220-223 bp ITS2 gene fragments and 5.8S rDNA were obtained from 13 samples of Salvia L. The interspecific substitution varied from 0% to 12.16% at ITS1 and 0.5% to 13.51% at ITS2. The intraspecific substitution of RRSM is 0% to 0.9% at ITS1 and 0% to 0.5% at ITS2. The substitution between group of Salvia L. and outgroup varied from 16.07% to 20.27% at ITS1 and 15.91% to 20.27% at ITS2. The phylogenetic tree based on ITS1 and ITS2 data was set up. Conclusion The ITS1 and ITS2 gene fragments are highly conservative at intraspecific level in RRSM, while they are less conservative at interspecific level in Salvia L. They are least conservative between group of Salvia L. and outgroup. Hence, the sequence of this fragment is a good molecular marker for authentication of RRSM and can be used for further study on the phylogenesis for plants of Salvia L.