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Objective To study the effect of Xiaoyan Decoction on metastasis of non-small cell lung cancer by experimental study. Methods Through the scratch test, the effect of Xiaoyan Decoction on the migration ability of lung adenocarcinoma A549 cells was observed. The transwell migration assay was used to test the role of Xiaoyan Decoction in lung cancer stem cells migration. Results In the scratch test, the migration distance of lung adenocarcinoma A549 cells in 24, 48, and 72 h was significantly shorter than that of the control group at the time of the scratch test (P < 0.05). In the invasive experiment, the number of lung cancer stem cells passing through the transwell unit in Xiaoyan Decoction group was significantly less than that of the control group (P < 0.05). Conclusion Xiaoyan Decoction can effectively inhibit the invasion ability of lung cancer stem cells, and then affect their metastatic ability.
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Objective:To investigate the influence of miR-7 knock down on the development of CD 4+SP cells in the thymus in mice,and preliminary explore its possible mechanism.Methods:The changes of volume ,weight and total cell counts of thymus in miR-7 knock down (miR-7KD) mice were observed compared with Wild-type(WT)mice;the pathological changes of thymus were observed by HE staining.FACS analysis was performed on the proportion ,as well as the expression level of CD44 and CD62L,of thymus CD4+single positive (SP) cells.Meanwhile,the proliferation percentage of CD4+SP cells was measured by Ki-67 staining.The apoptosis percentage of CD4+SP cells was analyzed by FACS.The changes on the transduction of ERK 1/2 pathways were determined by Western blot.Results:Compared with WT mice ,the size,weight and total cell number of thymus were marked reduced in miR-7KD mice( P<0.05 );moreover ,pathological change also was presented.The proportion and total cell number of thymus CD 4+SP cells were marked decreased ( P<0.05 ).Furthermore ,the expression level of CD 44 and proliferation percentage ,as well as apoptosis percentage ,of CD4+SP cells were obviously increased (P<0.05),however,the expression level of CD62L of CD4+SP cells were decreased (P<0.05). Finally,the level of total ERK1/2 and phosphor-ERK1/2 was decreased obviously ( P<0.05 ).Conclusion: miR-7 knock down can affect the development of CD 4+SP cells in the thymus , which might be closely related to the cell activation state and altered the transduction of ERK1/2 pathways.
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Objective: To evaluate the effect of curcumin on A549 tumor cell growth of subsets SP and NON-SP cells and the specific targets. Methods: The subsets SP and NON-SP cells were separated from A549 tumor strain of the human lung adenocarcinoma, and these cells were identified with soft agar clone formation test and tumorigenicity in vitro. Curcumin with different concentration (10, 20, 30, and 40 μmol/L) was used to deal with these cells. Using MTT method to make sure the inhibition of curcumin on the growth of SP and NON-SP cells. PCR was used to measure the expression of survivin and caspase-3. Results: Curcumin at different concentration showed the different inhibition on SP and NON-SP cells. Curcumin (30 μmol/L) showed more inhibition on SP cells than NON-SP cells with the lowest cell viability and was time-dependent. Curcumin could suppress the expression of survivin and promote the expression of caspase-3. The apoptosis degree of SP cell was clearly higher than that of NON-SP cell. Conclusion: Tumorigenicity of SP cell is higher than that of NON-SP cell. Curcumin induces the apoptosis of tumor cells through inhibiting the growth of SP cell, down-regulating the expression of survivin, and promoting the expression of caspase-3 gene.
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Side population (SP) cells are highly enriched for stem cell activity and characterized by their ability to efflux the vital dye Hoechst 33342, because they express the ATP binding cassette (ABC)-dependent transporter ABCG2. SP cells can be selected from main population using flow cytometric analysis. Currently SP cells have been isolated from many tissues and organs. SP cells of different origins have some common characteristics. This article introduces the classifications, surface marker, and characteristics of SP cells.
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Objective Comparing biological characteristics and the roles in repairing of intestinal irradiation injury of bone marrow derived Flk-1+ MSCs and small intestinal derived SP cells.Methods Phenotypes of the two cell population were determined by flow cytometery.Flk-1+ MSCs and intestinal SP cells were infused into irradiated mice,distribution and differentiation of these cells were determined.Histopathological change and fibrosis were evaluated.Results The phenotypes of Flk-1+ MSCs and intestinal SP cells were quite different.Both Flk-1+ MSCs and intestinal SP cells could home to injury tissue rapidly,participate intestinal epithelial repair,and alleviate fibrosis and collagen deposition after irradiation.SP cells mostly engrafted into intestinal epithelium,while Flk-1+ MSCs partly engrafted into interstitial region as well as epithelium.Conclusion Flk-1+ MSCs and intestinal SP cells may alleviate symptoms effectively,though their mechanisms are disparite.
Résumé
Side population (SP) cells are highly enriched for stem cell activity and characterized by their ability to efflux the vital dye Hoechst 33342,because they express the ATP binding cassette (ABC)-dependent transporter ABCG2.SP cells can be selected from main population using flow cytometric analysis.Currently SP cells have been isolated from many tissues and organs.SP cells of different origins have some common characteristics.This article introduces the classifications,surface marker,and characteristics of SP cells.