Establishment of a transgenic zebrafish line with tdTomato expression in the ultraviolet-sensitive cone photoreceptors / 中国实验动物学报

Objective To better understand the mechanisms of cone opsin transport , we set to generate a trans-genic zebrafish line with red fluorescence proteins expressing in the cone photoreceptors .Methods We used sws1 promot-er to drive the expression of a chimerical protein , in which the last 44 amino acids of rhodopsin of Xenopus laevis were fused to the C-terminus of tdTomato to restrict its localization to the outer segment of photoreceptors .Results We successfully i-solated such a transgenic zebrafish line and confirmed the localization of tdTomato by immunostaining analysis .Conclu-sions This transgenic zebrafish line will help us to better understand the transport mechanisms of cone opsins , especially the transport in live photoreceptors .

Sleep, ageing and night work

Studies have shown that the frequency or worsening of sleep disorders tends to increase with age and that the ability to perform circadian adjustments tends to decrease in individuals who work the night shift. This condition can cause consequences such as excessive sleepiness, which are often a factor in accidents that occur at work. The present study investigated the effects of age on the daytime and nighttime sleep patterns using polysomnography (PSG) of long-haul bus drivers working fixed night or day shifts. A total of 124 drivers, free of sleep disorders and grouped according to age (<45 years, N = 85, and ≥45 years, N = 39) and PSG timing (daytime (D) PSG, N = 60; nighttime (N) PSG, N = 64) participated in the study. We observed a significant effect of bedtime (D vs N) and found that the length of daytime sleep was shorter [D: <45 years (336.10 ± 73.75 min) vs N: <45 years (398 ± 78.79 min) and D: ≥45 years (346.57 ± 43.17 min) vs N: ≥45 years (386.44 ± 52.92 min); P ≤ 0.05]. Daytime sleep was less efficient compared to nighttime sleep [D: <45 years (78.86 ± 13.30 percent) vs N: <45 years (86.45 ± 9.77 percent) and D: ≥45 years (79.89 ± 9.45 percent) and N: ≥45 years (83.13 ± 9.13 percent); P ≤ 0.05]. An effect of age was observed for rapid eye movement sleep [D: <45 years (18.05 ± 6.12 percent) vs D: ≥45 years (15.48 ± 7.11 percent) and N: <45 years (23.88 ± 6.75 percent) vs N: ≥45 years (20.77 ± 5.64 percent); P ≤ 0.05], which was greater in younger drivers. These findings are inconsistent with the notion that older night workers are more adversely affected than younger night workers by the challenge of attempting to rest during the day.

A Case of Pyogenic Granuloma within Port-Wine Stain Associated with Sturge-Weber Syndrome / 대한피부과학회지

Port-wine stains(PWS) are congenital vascular birthmarks and usually isolated but may be associated with other vascular malformations. Sturge-Weber syndrome(SWS) is defined as facial PWS in association with an ipsilateral leptomeningeal vascular malformation, atrophy and calcification of the underlying cerebral cortex, epilepsy, mental retardation, contralateral hemiparesis and ipsilateral choroidal vascular lesions with glaucoma. Pyogenic granulomas or other nodular angiomatous lesions may develop within preexistent vascular malformation such as PWS. We describe a 22 year-old man who developed a pyogenic granuloma within an existing facial PWS associated with SWS.

A Case of Pyogenic Granuloma within Port-Wine Stain Associated with Sturge-Weber Syndrome / 대한피부과학회지

Port-wine stains(PWS) are congenital vascular birthmarks and usually isolated but may be associated with other vascular malformations. Sturge-Weber syndrome(SWS) is defined as facial PWS in association with an ipsilateral leptomeningeal vascular malformation, atrophy and calcification of the underlying cerebral cortex, epilepsy, mental retardation, contralateral hemiparesis and ipsilateral choroidal vascular lesions with glaucoma. Pyogenic granulomas or other nodular angiomatous lesions may develop within preexistent vascular malformation such as PWS. We describe a 22 year-old man who developed a pyogenic granuloma within an existing facial PWS associated with SWS.

Effects of total glucosides of paeony on sleep-waking rhythm in rats / 中国药理学通报

Total glucosides of paeony (TGP, 50 mg/kg 7d, ig) could enhance the episode duration of slow-wave sleep (SWS) in normal rats, and restore the sleep parameters in the insomniac rats induced by caffeine ( 1 2. 5 mg/kg 7 d,ip)nearly to the normal level. It (50 mg/kg 3 d,ig) also increased significantly thetotal time of SWS and paradoxical sleep (PS) in the swimming rats (water temperature 25?1℃, swimming time 30 min). These results suggest that TGP probably improve the sleep of rats under the different states.