Effects of Salacia Oblonga on aluminium induced changes on membrane ATPases of various organs in Wistar albino rats

Background: Aluminium, one of the abundant elements present in nature enters human body through diet, food packaging material and medication. Aluminium can induce toxicity by causing oxidative damage and by altering the function of membrane bound ATPases, Salacia oblonga, a woody climbing plant with vast geographical distribution in India possess various biological functions. Previous studies have shown a protective effect of S. oblonga on Al induced visceral toxicity. Our aim is to evaluate the effect of S. oblonga extract on aluminium induced oxidative organ damage and haematological changes Methods: The experimental animals, Wistar albino rats, were divided into four groups of 6 per group. The four groups are divided into distilled water, Aluminium chloride, Salacia oblonga extract and aluminium and Salacia oblonga. On 37th day animals were sacrificed and organs and blood were collected for assessment of ATPase activity, protein levels and haematological parameters. Results: Salacia oblonga increased the ATPase activity in the brain, heart and lungs, improved the protein levels and haematological parameters which were deteriorated by Al toxicity. Though statistically insignificant S. oblonga improved the ATPase enzymes and haematological parameters. Conclusions: Our study demonstrated that Aluminium causes membrane damage in various organs by altering the membrane ATPases level as a result of oxidative stress and altered the haematological parameters. We demonstrated that S. oblonga ameliorates Al induced membrane toxicity and also confers hemoprotective effect.

Effects of Salacia oblonga on aluminium induced changes in membrane ATPases in brain cortex and hematological parameters in Wistar albino rats

Background: The current study was done to evaluate the effect of hydro alcoholic extract of Salacia oblonga on aluminum induced toxicity in brain cortex and blood in Wistar albino rats.Methods: The experimental animals were divided into four groups, each group comprising of six animals for 36 days of experimental duration. We investigated Na+/K+, Mg2+, Ca2+ ATPases enzyme activity in brain cortex and hematological changes if any, upon administration of aluminum chloride (Alcl3) (300 mg/kg b.w), hydro alcoholic extract of Salacia oblonga (67 mg/kg b.w) and Alcl3+ Salacia oblanga with control (distilled water).Results: In brain cortex, Salacia caused an increased in activity of ATPases. Combined administration of Salacia suppressed the influence of aluminum on the ATPases in the brain cortex (p<0.05). Both aluminum as well as Salacia did not cause any alteration in the hemoglobin content of blood in Wistar albino rats. The erythrocytes count was also not altered by treatment with either aluminum or Salacia. Combined treatment with Salacia suppressed the influence of aluminum with reference to neutrophil count and significant increases in monocyte as well as lymphocyte count were seen. Thus, repeated administration of aluminum causes a decrease in neutrophil and increase in lymphocyte count.Conclusions: All the ATPases in brain cortex were found to be affected by aluminum administration and Salacia is found to counteract the ATPase effect to a particular extent implying the presence of an active principle that can counteract the aluminium toxicity indicating its possible usefulness in aluminum toxicity.

Investigation of Mode of Action of Anti Bacterial Activity of Salacia Oblonga Extract Against Drug Resistant Pathogen

Abstract Salacia oblonga Wall belonging to family Celastraceae contains vital phytoconstituents and has been used since long for the treatment of diabetes, inflammation and burn wounds. S. oblonga ethyl acetate root extract was evaluated for antibacterial activity towards drug resistant pathogens Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Further 260 nm absorbing material was estimated in the control and treated cells. Interestingly 260 nm absorbance material is higher in the Staphylococcus aureus. Further the effect of the plant extract on drug resistant pathogen S. aureus was examined by scanning electron microscopy (SEM). SEM results have shown that treated bacterial cells have changed in morphology, size and reduced in number. Based on these results it can be concluded that S. oblonga extract acts on membrane of the drug resistant pathogen S. aureus.

Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis

ABSTRACT Our present investigation deals with the phytochemical screening, estimation of total flavonoids, terpenoids and tannin contents to evaluate the anti-diabetic activities of Salacia oblonga stem followed by GC-MS analysis. It explores the natural compounds and the potential α-amylase and α-glucosidase inhibitory actions of stem extracts. The aqueous stem extract was selected from other extracts (ethanol, acetone, petroleum ether and chloroform) for the in vitro study of anti-diabetic activity by alpha amylase and alpha glucosidase inhibitory assays. The stem extract was also analyzed by gas chromatography mass spectrometry to identify the natural chemical components. Phytochemical analysis of aqueous stem extract showed major classes of secondary metabolites such as phenols, flavonoids, alkaloids, terpenoids, tannins, saponins. The total flavonoid, terpenoid, and tannin contents were quantified as 19.82±0.06 mg QE/g, 96.2±0.20 mg/g and 11.25±0.03 mg TAE/g respectively. The percentage inhibition of assays showed maximum inhibitory effects (59.46±0.04% and 68.51±0.01%) at a concentration of 100 mg/mL. The IC50 values of stem extract was found to be 73.56 mg/mL and 80.90 mg/mL for alpha amylase and alpha glucosidase inhibition. Fifteen chemical constituents were found by GC-MS analysis. This study suggest the aqueous stem extract of Salacia oblonga might be considered as potential source of bio active constituents with excellent antidiabetic activity.

Evaluation of antioxidant effect of Salacia oblonga against aluminum chloride induced visceral toxicity in albino rats.

Background: Aluminum is present in several manufactured foods and medicines and is also used in water purifi cation. It is known that aluminum induces an oxidative stress characterized by an increase in lipid peroxidation and depletion of antioxidants. Therefore, the present experiment was undertaken to determine the effectiveness of hydroalcoholic extract of root bark of Salacia oblonga (SOHE) in modulating the aluminum chloride (AlCl3) induced oxidative stress in rats. Methods: Animals were assigned into four groups: control; AlCl3 300 mg/kg b.w.; Salacia 67 mg/kg; AlCl3 (300 mg/kg b.w.) plus Salacia (67 mg/kg b.w.), respectively. Rats were orally administered their respective doses daily for 36 days. The effect of these treatments in infl uencing the aluminum induced biochemical changes on liver, kidney, lungs, and heart were studied. Result: The results showed that S. oblonga produced signifi cant (p<0.05) reduction of malondialdehyde, while the activities of superoxide dismutase, reduced glutathione, glutathione S-transferase and catalase were positively modulated. Conclusion: Our data demonstrated that S. oblonga protects against aluminuminduced oxidative stress, which is an important fi nding that further reinforces the antioxidant properties of this natural product.