Résumé
OBJECTIVE To investigate the pharmacological effect and mechanism of Sanguisorba officinalis L. (SOL) in non-small cell lung cancer (NSCLC) in vitro and in vivo based on network pharmacology. METHODS Network pharmacology was used to analyze the improving effect of SOL on NSCLC and possible targets. Cell counting kit 8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) staining, Western blotting, flow cytometry of Annexin Ⅴ/PI, Hoechst 33342/PI staining detection and immunofluorescence were utilized in vitro. H&E staining, immunohistochemistry staining and Western blotting were performed in vivo. RESULTS Based on network prediction, we analyzed the 208 common targets of SOL and NSCLC. 36 core targets in 208 common targets were obtained through cytoscape analysis. And the top 10 core targets included Akt, mTOR, EGFR, etc.. KEGG analysis showed that PI3K-Akt signaling pathway was the most likely pathway. Furthermore, the mechanism study found that SOL could activate the PI3K/Akt/mTOR signaling pathway in vitro and in vivo. The anti-proliferative effect of SOL in A549 and H1299 cells was measured and validated by CCK-8 and EdU assay. Immunohistochemical results of Ki67 showed that SOL effectively inhibited tumor growth in vivo. SOL also significantly inhibited the migration and invasion of A549 and H1299 cells. SOL significantly increased the percentage of cells with PI signal in A549 and H1299, and the process of cell death of A549 cells indicated that SOL induced apoptosis. The PARP-1 and caspase-3 in A549 and H1299 were found to be activated in a dose manner. The results in vivo were consistent with those in vitro. CONCLUSION SOL-induced, caspase-3-mediated apoptosis via the induction of the PI3K/Akt/mTOR signaling pathway in NSCLC, which further clarified the mechanism of SOL in the inhibition of NSCLC, and thereby provided a possibility for SOL to serve as a novel therapeutic agent for NSCLC in the future.
Résumé
Objective: To study the chemical constituents in extract of Sanguisorba officinalis. Methods: Compounds were isolated from 10% ethanol extracts by Macroporous resin (D101, HP-20), dialysis bag, reversed phase silica gel (RP-8, RP-18), Toyopearl HW-40 column chromatography and their structures were elucidated by NMR and MS data. Results: Twenty-five compounds were isolated and their structures were elucidated as 4-(4’-hydroxyphenyl)-2-butanone-4’-O-β-D-glucopyranoside (1), phenethanol-β- vicianoside (2), junipetrioloside A (3), citroside A (4), corchoionoside C (5), adenosine (6), tryptophan (7), tachinoside (8), d-mandelic acid-β-D-glucopyranoside (9), (+) (7S,8S)-guaiacylglycerol 8-O-β-D-glucopyranoside (10), biophenol (11), 3,5-dihydroxyphenethyl alcohol 3-O-β-glucopyranoside (12), syringin (13), (2E,5E)-3,7-dimethyl-2,5-octadiene-1,7-diol (14), (±)-3-hydroxy-3,7-dimethyloct- 6-enoic acid (15), (2Z)-2,6-dimethyl-2,7-octadiene-1,6-diol (16), phlorizin (17), (+)-cyclo-olivil 6-O-β-D-glucopyranoside (18), 5’-methoxy-8’-hydroxyl-(+)-isolariciresinol-4’-O-β-D-gluco-pyranoside (19), phenethyl 6-O-α-L-arabinofuranosyl-β-D-glucoside (20), gaultherin (21), benzyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (22), (2E)-7-hydroxy-3,7-dimethyl-2-octenyl 6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (23), 3,3’,4’-tri-O-methylellagic acid (24), and methyl-4-(β-D-glucopyranosyloxy)- 3-hydroxy-5-methoxybenzoate (25). Conclusion: Compounds 1-22 were isolated from this plant for the first time.
Résumé
Objective: To study the in vitro antilipid-peroxidation effects of tannins extract from Sanguisorba officinalis on heart, liver, brain and kidney of rats.Methods: The in vitro inhibitory effects of tannins extract from Sanguisorba officinalis on lipid-peroxidation of heart, liver, brain and kidney of rats induced by Fe2+-cysteine (Fe2+-Cys), Fe2+-vitamin C (Fe2+-Vit C) and Fe2+-H2O2 were determined by spectrophotometry.Results: The inhibitory effects of tannins extract from Sanguisorba officinalis on MDA produced by lipid-peroxidation of brain, heart, liver homogenate, kidney and mitochondria of rats induced by Fe2+-Cys, Fe2+-Vit C and Fe2+-H2O2 were all significant.Conclusion: Tannins from Sanguisorba officinalis has good in vitro protective effects on antilipid-peroxidation of heart, liver, brain and kidney of rats, which is worth studying further.
Résumé
Objective To investigate inhibitory effects of Sanguisorba officinalis L.on biofilms of MRSA41577.Methods Congo red agar method and crystal violet semi-quantitative method were used to detect the biofilms-forming ability of tested strains; TTC assay was used to detect inhibitory effects of Sanguisorba officinalis L.on biofilms formation and mature biofilms of MRSA41577,as well as effects of Sanguisorba officinalis L.in combination with vancomycin on mature biofilms of MRSA41577.Results Sanguisorba officinalis L.showed significant inhibitory both on biofilms formation and mature biofilms, minimum inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of biofilms formation were 1 mg/mL and 8 mg/mL,MIC of mature biofilms was 4 mg/mL.The sensitivity of mature biofilms to vancomycin was greatly increased when Sanguisorba officinalis L.was combined with vancomycin with subinhibitory concentrations.Sanguisorba officinalis L.at 1/4 MIC can inhibit mature biofilms when combined with vancomycin at 4 μg/mL, while vancomycin didn't show inhibitory effects on mature biofilms when concentrations were below 64 μg/mL. Conclusion Sanguisorba officinalis L.has significant inhibitory on biofilms formation, the mechanism may be related to Sanguisorba officinalis L.destroyed biofilms and make vancomycin penetrate into the biofilms to finish the bactericidal activity.