Résumé
Objective: To study the chemical constituents of Sedum aizoon and to screen the anti-oxidant activities and α-glucosidase inhibitory activities of compounds. Methods: The compounds were separated and purified by various chromatographic techniques, and their structures were identified by physio-chemical properties and EI-MS, ESI-MS, 1H and 13C NMR. The anti-oxidant activity of compounds was screened by DPPH method. The obtained compounds were subjected to detection of α-glucosidase inhibitory activity by PNPG method. Results: Seventeen compounds were isolated from S. aizoon, which were identified as iriflophene (1), kaempferol (2), quercetin (3), myricetin (4), rhamnazin-3-O-β-D-glucopyranoside (5), isolariciresinol-9-O-β-D-glucopyranoside (6),myricitrime (7), myricetin-3-O-α-L-arabinopyranoside (8), iriflophenone-2-O-β-D-glucopyranoside (9), 2-O-(trans-caffeoyl)-malic acid 1-methyl-ester (10), 2-O-(trans-caffeoyl)-malic acid 1,4-dimethyl ester (11), 2-O-(trans-caffeoyl) malic acid (12), p-coumaric acid (13), ethyl gallate (14), butanedioic acid (15), 9(Z)-octadece-namide (16), and lotaustralin (17). Conclusion: Compounds 13 and 15 are isolated from S. aizoon for the first time. Compounds 9-12, and 16 are isolated from genus Sedum for the first time. Compounds 2, 3, 7, 8, 10, 12, and 14 had significant anti-oxidant activity. Compounds 8 showed moderate α-glucosidase inhibitory activity in vitro. Compound 3 showed strong α-glucosidase inhibitory activity in vitro.
Résumé
Objective:The fingerprint chromatograms were established for quality evaluation of Sedum aizoon L.collected from different habitats by HPLC.Methods:The analysis was performed on a Diamonsil C18 column(4.6mm? 250mm,5?m)with acetonitrile-water(acidified to 0.5%with phosphoric acid)as mobile phase in a gradient mode at a flow rate of 1.0 mL/min,and at a column temperature of 25℃.The detection of wavelength was at 254 nm.Results:2lpeaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 3%in the precision and repeated test.The similarity of l0 batches of samples were no less than 0.9.Conclusion:The method was reliable and can be helpful on the quality control of Sedum aizoon L.
Résumé
Objective: To establish the quatity standard of Sedum aizoon L..Methods: The micro-method and TLC were used for qualitative identification,and a HPLC analysis was applied for quantitative determination of quercetin.Results: A qualitative analysis for Sedum aizoon L.was set up,a good linear relationship was obtained over the range of 1.216-2.16?g/ml and regression equation was Y=40386X-14138(r=0.9991).The average recovery rate was 102.08%(RSD=0.92%).Conclusion: The method is simple,accurate and suitable for quality control of Sedum aizoon L.
Résumé
Objective: To discover and determine the content of kaempferol in Sedum aizoon L.for the first time.Methods: Waters HPLC-MS/MS,XTerra-MS C18 (5?m,2.1?150mm) and the mobile phase consisted of acetonitrile-water-formic(40∶60∶1) were applied to find kaempferol in Sedum aizoon L.;Daojin HPLC and the SHIM-PACK VP C18(250nm?4.6nm,10?m) column were used.The mobile phase consisted of methanol-0.4% phosphoric acid solutions(59:41) with the flow rate at 1.0ml/min and the UV detector wave-length were set at 370nm.Results: Compared with standard sample,the thing that kaempferol exists in Sedum aizoon L.was confirmed.The calibration curve was in good linearity over the range of 2.0-8.0?g,and regression equation was Y=40343X-11107(r=0.9998).The average recovery rate was 102.53%,with RSD =0.92%(n=6).Conclusion: The method is simple,accurate and reproducible so it can be used to determine and analyze the content of kaempferol in Sedum aizoon L.
Résumé
AIM: To develop the methods for the quantitative analysis of gallic acid and total phenolic acid in Sedum Aizoon L. METHODS: Gallic acid was analyzed by HPLC on a Hypersil BDS C_(18) column and detected at 271 nm.The mobile phase was methol-water(adjusted to pH=3.0 with H_3PO_4)(90∶10)at a flow rate of 1.0 mL/min.Total phenolic acid was analyzed by spectrophotometry at 720 nm.The colour-developing agent was the mixture solution of 0.6%FeCl_3—0.9%K_3[Fe(CN)_6](1∶0.9). RESULTS: Calibration graphs were constructed in the range of 0.343 0-1.200 ?g(r=0.999 7) for gallic acid and 0.4640-2.320 ?g/mL(r=(0.999 3)) for total phenolic acid.The average recoveries were 97.91%(n=6) for gallic acid and 99.21%(n=6) for total phenolic acid.The RSD of the methods were 1.8% and 2.0%,respectively. CONCLUSION: The methods were fast,reliable,accurate and suitable for analysis of gallic acid and total phenolic acid and quality control in Sedum Aizoon L.