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1.
Rev. Soc. Bras. Med. Trop ; 47(2): 186-192, Mar-Apr/2014. tab, graf
Article Dans Anglais | LILACS | ID: lil-710358

Résumé

Introduction Açucena Municipality, Rio Doce Valley, State of Minas Gerais, Brazil temporarily (2001-2005) interrupted epidemiological surveillance for Chagas disease. The objective of this work was to evaluate the Chagas Disease Control Program (CDCP) in Açucena and to offer suggestions for improving local epidemiological surveillance. Methods This study was conducted in three phases: I) a serological investigation of schoolchildren aged 5 to 15 years using an enzyme-linked immunosorbent assay (ELISA) test performed on blood collected on filter paper followed by ELISA, indirect immunofluorescence (IIF) and indirect hemaglutination (IHA) on venous blood for borderline cases and those in the gray zone of reactivity; II) vector evaluation using the data obtained by local health agents during 2006-2010; and III) examination by ELISA, IIF and IHA of serum samples from the inhabitants of houses where infected Triatoma vitticeps was found and evaluation of their knowledge about Chagas disease. Results Five individuals had inconclusive results in the ELISA screening but were seronegative for Chagas disease. The triatomine evaluation revealed the presence of three species: Triatoma vitticeps, Panstrongylus megistus and Panstrongylus diasi. Triatoma vitticeps was the most prevalent and widespread, with a higher (67%) index of Trypanosoma cruzi flagellates and evidence of colonization. Most of the inhabitants of the infested houses recognized triatomines and had basic knowledge about Chagas disease. Conclusions Although T. vitticeps is not clearly associated with Chagas disease transmission, these results highlight the importance of maintaining CDCP in endemic areas and the need for greater emphasis on epidemiological surveillance, especially in areas with important vectorial changes or that have been modified by human intervention. .


Sujets)
Adolescent , Animaux , Enfant , Enfant d'âge préscolaire , Humains , Maladie de Chagas/prévention et contrôle , Lutte contre les insectes , Vecteurs insectes/classification , Trypanosoma cruzi/immunologie , Brésil/épidémiologie , Maladie de Chagas/diagnostic , Maladie de Chagas/épidémiologie , Test ELISA , Technique d'immunofluorescence indirecte , Tests d'hémagglutination , Logement , Vecteurs insectes/parasitologie , Surveillance de la population , Prévalence , Évaluation de programme , Panstrongylus/classification , Panstrongylus/parasitologie , Triatoma/classification , Triatoma/parasitologie
2.
Asian Pacific Journal of Tropical Biomedicine ; (12): 102-103, 2012.
Article Dans Chinois | WPRIM | ID: wpr-499754

Résumé

Objective:To evaluate the Anaplasma phagocytophilum (A. phagocytophilum), Ehrlichia canis (E. canis), Dirofilaria immitis (D. immitis) (canine heartworm), Borrelia burgdorferi (B. burgdorferi) infections in countryside dogs from Yunnan, Hainan and Anhui provinces. Methods: Serum samples were collected from 26 dogs in Yunnan, Hainan and Anhui provinces. The samples were tested using a commercial ELISA rapid diagnostic assay kit (SNAP? 4Dx?; IDEXX Laboratories, Inc. U.S.A.). Meanwhile, indirect immunofluorescence assay (IFA) recommended by WHO was conducted to detect IgG to A. phagocytophilum. Two methods were analyzed and compared. Results: The number of serologically positive dogs for IgG to A. phagocytophilum was only 2 which was from Hainan province and none of the 26 dogs responded positive for E. canis, D. immitis (canine heartworm), and B. burgdorferi by ELISA rapid diagnostic method. The number of serologically positive dogs for IgG to A. phagocytophilum was 13 (50%) by IFA method. Data of the two methods were analyzed by statistical software and the difference was statistically significant (P=0.002). Conclusions: It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method. However, we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including A. phagocytophilum, E. canis, D. immitis (canine heartworm), and B. burgdorferi which have public health significance.

3.
Asian Pacific Journal of Tropical Biomedicine ; (12): 102-103, 2012.
Article Dans Anglais | WPRIM | ID: wpr-303615

Résumé

<p><b>OBJECTIVE</b>To evaluate the Anaplasma phagocytophilum (A. phagocytophilum), Ehrlichia canis (E. canis), Dirofilaria immitis (D. immitis) (canine heartworm), Borrelia burgdorferi (B. burgdorferi) infections in countryside dogs from Yunnan, Hainan and Anhui provinces.</p><p><b>METHODS</b>Serum samples were collected from 26 dogs in Yunnan, Hainan and Anhui provinces. The samples were tested using a commercial ELISA rapid diagnostic assay kit (SNAP(®) 4Dx(®); IDEXX Laboratories, Inc. U.S.A.). Meanwhile, indirect immunofluorescence assay (IFA) recommended by WHO was conducted to detect IgG to A. phagocytophilum. Two methods were analyzed and compared.</p><p><b>RESULTS</b>The number of serologically positive dogs for IgG to A. phagocytophilum was only 2 which was from Hainan province and none of the 26 dogs responded positive for E. canis, D. immitis (canine heartworm), and B. burgdorferi by ELISA rapid diagnostic method. The number of serologically positive dogs for IgG to A. phagocytophilum was 13 (50%) by IFA method. Data of the two methods were analyzed by statistical software and the difference was statistically significant (P=0.002).</p><p><b>CONCLUSIONS</b>It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method. However, we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including A. phagocytophilum, E. canis, D. immitis (canine heartworm), and B. burgdorferi which have public health significance.</p>


Sujets)
Animaux , Chiens , Anaplasma phagocytophilum , Allergie et immunologie , Borrelia burgdorferi , Allergie et immunologie , Chine , Épidémiologie , Dirofilaria immitis , Allergie et immunologie , Dirofilariose , Sang , Épidémiologie , Allergie et immunologie , Vecteurs de maladies , Maladies des chiens , Épidémiologie , Ehrlichia canis , Allergie et immunologie , Ehrlichiose , Sang , Épidémiologie , Allergie et immunologie , Test ELISA , Méthodes , Technique d'immunofluorescence indirecte , Méthodes , Immunoglobuline G , Sang , Maladie de Lyme , Sang , Épidémiologie , Allergie et immunologie , Maladies transmises par les tiques , Épidémiologie
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