Résumé
Objective: To optimize the formula of artificial diets for Poecilobdella manillensis. Methods: Survival rate and individual body weight growth rate of P. manillensis were chosen as indexes. The three factors [NP-90 spray-dried hemoglobin powder, NP-2002 spray-dried plasma powder, and vitamin C (VC)] of the formula of artificial diets were investigated by L9(34) orthogonal test. AHP was applied to statistical analysis on non-interacting orthogonal test, the effect weight of each factor for test result was obtained, and principal-secondary sequence of factors and optimal project was given. Results: The effects of the three components in artificial diets on the survival rate were in the following decreasing order: VC>NP-90>NP-2002. And the optimization of components of artificial diets was 0 NP-90 spray-dried hemoglobin powder, 1% NP-2002 spray-dried plasma powder, and 0.05% VC. When the individual body weight growth rate was chosen as the index, the effects of the three components in artificial diets on the individual body weight growth rate were in the following decreasing order: NP-2002>NP-90>VC. The optimization of components of artificial diets was 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, and 0.05% VC. The weight of principal-secondary sequence of factors and levels influences on the tests' results was NP-90>NP-2002>VC. The weight of 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder and 0.05% VC was maximum. And the optimization of components in artificial diets was 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, and 0.05% VC. According to the results, P. manillensis bred with the artificial diets (0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, 3% NP-2002 spray-dried plasma powder, 0.05% VC, and other components) had the survival rate of 100% and the individual body weight growth rate was 19.91%. Conclusion: The optimal artificial diets could be used to feed P. manillensis.
Résumé
Foram avaliados os efeitos do plasma sanguíneo desidratado (PSD) sobre desempenho, perfil imunológico, histológico, microbiológico e peso de órgãos de leitões leves, desmamados aos 21 dias de idade. Foram utilizados 24 leitões, com idade média inicial de 21 dias, em delineamento experimental completamente ao acaso. Os tratamentos foram: T1 - animais pesados ao desmame, sem suplementação com PSD; T2 - animais leves ao desmame, suplementados com 10g/animal/dia de PSD; T3 - animais leves ao desmame, suplementados com 20g/animal/dia de PSD; T4 - animais leves ao desmame, sem suplementação com PSD. A adição de 20g de PSD na dieta melhorou o ganho diário de peso, aumentou o peso (g/kg) do baço e o título de IgA no soro entre 21 e 31 dias de idade. A inclusão de 10g de PSD aumentou o comprimento e a largura do linfonodo ileocólico. A inclusão de PSD traz benefícios aos leitões nos primeiros 10 dias pós-desmame, atuando principalmente nos órgãos linfoides e na mucosa intestinal.
The aim of this experiment was to evaluate the effects of spray-dried plasma (SDP) on the growth performance, immunological, histological and microbiological profile and weight of organs of light weight weaned pigs. The trial was done using 24 pigs with an initial mean age of 21 days in a completely randomized experimental design. The treatments were: T1 - heavy weight weaned pigs, without SDP supplementation; T2 - light weight weaned pigs, supplemented with 10g/animal/day of SDP; T3 - light weight weaned pigs, supplemented with 20g/animal/day of SDP; T4 - light weight weaned pigs, without SDP supplementation. The inclusion of 20g of SDP in the diet improved the weight gain, spleen weight (g/kg) and serum IgA title between 21 and 31 days of age. The inclusion of 10g of SDP in the diet improved the length and width of the ileocolic lymph node. In the first 10 days after weaning, SDP improved the development of lymphoid organs and the protection of the intestinal mucosa.