Comparison of Class 5 and 6 chemical indicative cards applied to pressure steam sterilization in hospitals / 医疗卫生装备

Objective To observe the effects of Class 5 and 6 chemical indicative cards used for monitoring pressure steam sterilization to standardize the procedure for selecting the monitoring substance of pressure steam sterilization. Methods Several pieces of Class 5 and 6 cards were placed at the four corners and center of the clave to undergo sterilization with the conventional parameters of 134 ℃, 240 s and 220 kPa as well as the simulation critical parameters of 134 ℃, 120 s and 220 kPa.The two kinds of cards had their qualification rates compared with two sets of parameters.SPSS 19.0 software was used for data analysis and Chi-square test was applied to comparison.Results The two kinds of cards had the qualification rates being 100% with the conventional parameters;with the simulation critical parameters,Class 5 card had the qualification rate being 100%,while Class 6 card had the rate of 66.67%,and there were significant difference between the qualification rates of the two cards(P<0.05).Conclusion Class 6 chemical indicative card has higher resistance and efficiency with the same conditions when compared with Class 5 card,which can be combined with the tube cavity challenger to execute mass monitoring with unstable parameters.

The effectiveness evaluation of three packing methods for controlling sterilization wet package of medical apparatus by using non-woven fabrics / 中国医学装备

Objective: To explore the drying effect of pressure steam sterilization under different packing planes of non-woven fabrics so as to avoid the phenomenon of wet packages. Methods: 600 apparatus packages that packed by using non-woven fabrics were divided into A group(the packaging plane of apparatus bag used convex shape, that the center was higher and the periphery was lower), B group(The packaging plane of apparatus bag used horizontal shape) and C group (The packaging plane of apparatus bag used concave shape, that the center was lower and the periphery was higher)as random number table. All of the three groups adopted same package (the inner package was cotton and the outside package used non-woven fabrics of double-deck). Through pressure steam of impulse vacuum to achieve sterilization, and then the wet package rates of three groups were compared. Results: The wet package rates of the three groups were 1%, 8.5% and 37.5%, respectively, and the differences of wet package rates among the three groups were statistically significant (x2=10.135, P<0.05). Conclusion: The wet package rate of A group was lower than that of B group and C group, respectively. Therefore, this method of A group can ensure drying effect and guarantee the safety of clinical use.

Effect of loading density of different rigid containers on wet package of orthopedic instruments in the orthopedics department / 现代临床护理

Objective To explore the effect of loading density of different rigid containers on wet package of orthopedic instruments in the department of orthopedics and to provide the basis for right loading of rigid container so as to ensure the success of the sterilization. Methods About 120 cases of orthopedic exotic instruments for knee surgery were selected from our hospital, according to size and weight of the knee surgery instrument and then evenly divided into Groups A, B, C, 40 pieces in each group. Each group for loading had the same length and width. In Group A, the loading was at 1/2 of the container height, in Group B, the loading was at 2/3 of the container height and in Group C the loading was 4/5 of the container height. The 3 groups were given high pressure steam sterilization, the sterilization temperature, sterilization time and drying time were the same, while biological and chemical monitoring was done. After sterilization, the three groups were compared in terms of the biological and chemical monitoring results as well as the incidence of wet pack. Results There were no significant differences between the 3 groups in the qualification rate of indicator cards and the biological monitoring after sterilization in the 5 kinds of packages among the 3 groups of rigid containers (P>0.05). The incidence of wet package in Group A was significantly lower than that of Group C (χ2=6.80, P=0.009<0.017), but no difference was found between Groups B and C ,Group B and C Group . Conclusions The loading density of different rigid containers affects the incidence of wet package of the orthopedic instruments. Our findings indicate that the loading of the instruments in a rigid container reaches the 2/3~4/5 height of the container, for it can reduce the incidence of wet package of orthopedic instruments.

Different disinfection solution on the disinfection effect analysis of the fiberoptic bronchoscopy biopsy forceps / 中国医学装备

Objective: Discuss different sterilization solution on the disinfection effect of fiberoptic bronchoscopy biopsy forceps. Methods:To ethylene oxide sterilization, glutaraldehyde immersion disinfection and high pressure steam sterilization of three methods of fiberoptic bronchoscopy biopsy forceps disinfection sterilization rate, the contamination rate and use fixed number of year for effect comparison. Results: Qualification rate of epoxy ethane sterilizing method were higher than in glutaraldehyde soaking method and high pressure steam sterilization disinfection method, and the pollution rate is significantly lower than the other two groups, the service life of the equipment increase. Conclusion:Low-temperature ethylene oxide sterilization of fiberoptic bronchoscopy biopsy forceps sterilization effect is good, and the pollution rate is low, service life is longer.

Observation of discoloration of chemical indicator cards in different positions in pressure steam sterilization bags and sterilization effect / 中国实用护理杂志

ObjectiveTo observe the discoloration of chemical indicator cards in different positions in pressure steam sterilization bags and stetilization effect.MethodsFrom January 2009 to January 2011,168 packages needing sterilization were divided into group A and group B randomiy with 84 packages in each.We put chemical indicator cards on different positions in the packages.Then discoloration of chemical indicator cards and the qualified rate of bacteriology detection were observed.ResultsThere were 9cases of uneven discoloration in group A,and the incidence rate was 10.71％,higher than group B.The unqualified rate of bacteriology detection in group A was 2.38％,higher than group B(1.19％),but the difference was not statistically significant.ConclusionsChemical indicator cards could reflect the effect of sterilization,but placing them in different positions can appear different results.The chemical indicator cards that placed between the two packing layer could provide powerful evidence for sterilization effects for clinicians,and avoid unnecessary economic waste in Sterilization and Supply Center,which is worthy of being widely used in Sterilization and Supply Center.

Chemical Monitoring Testing Package Application for High Pressure-steam Sterilization:An Observation and Analysis / 中华医院感染学杂志

OBJECTIVE To observe and compare the application result of two kinds of chemical indicator(CI)card contained in high pressure-steam sterilization chemical monitoring testing package for providing correct credible evidence of supplying material in each batch after high pressure-steam sterilization and to avoid resource lost because of fault estimation.METHODS The chemical monitoring testing package was made according Sterilization Criteria published in 2002,with the 3M 1250 and 1243 CI cards and 1292 biology indicator(BI) to observe the results in tested package after sterilization.RESULTS The BI in chemical monitoring testing package was all qualified,the qualified rate of 1250 was 70%,while of 1243 was 100%.CONCLUSIONS If there is no BI for monitoring,1243 CI card should be chosen in high pressure-steam sterilization chemical monitoring testing package for supplying material in each batch after high pressure-steam sterilization which is not influenced by moisture and the contacted material and is easy to read and evaluate,the resource lost caused by fault estimation can be avoided.

Influence of Various Packaging Methods and Controlling Method Toward Hidden Wet Pack after Steam Sterilization / 中华医院感染学杂志

OBJECTIVE To study the influence of various packing methods and controlling method toward the hidden wet pack after steam sterilization to ensure the sterilization quality and hygiene safety.METHODS The medical equipment was packed with three types of methods,then unloaded from the steam autoclave after being cooled at different time to observe their heating speed respectively.To choose two types of cotton fabric packaging methods,prolong the heating time,and cool in the steam autoclave for 30 minutes,then observe the heating speed of the inside pack and the dry qualification status after 12 hours respectively.RESULTS The heating speed was the fastest with the medical crinkly paper method;the heating speed of the inside pack didn't have any improvement after prolonging the heating time;the dry qualification rate had distinct difference between the two types of cotton fabric methods after 12 hours(P

Expiry Date of Sterile Articles Packed by 4 Different Materials and Preserved in Different Conditions after Pressure Steam Sterilizing: An Observation / 中华医院感染学杂志

OBJECTIVE To investigate the expiry date of sterile articles packed by 4 different materials and preserved in different conditions after pressure steam sterilizing. METHODS The bacteria growth of the materials sterilized by pressure steam sterilizing method and stored in the supply department,treatment room,dressing room,nursing cabinet and ambulance vehicle was observed. RESULTS The axenic periods of the sterile materials in the supply department preserved by methods A,B,C,D were 14 days,14 days,7 months and 8 months,respectively in summer;while the sterile periods of the materials in the treatment room,dressing room,nursing cabinet and ambulance vehicle preserved by methods A,B,C,D were 11 days,11days,6 months and 7 months,respectively. CONCLUSIONS Management of expiry date of sterile materials is an important measure to guarantee safe use of sterile materials and prevent against nosocomial infection.

Impact Factors on Sterilization Effect of Dental High-pressure Steam Sterilizer / 中华医院感染学杂志

OBJECTIVE To study the influence to the sterilizing effect of these factors,which were preheating the sterilizer,the positions of the articles placed and the cleanness of the internal wall.METHODS A total of 120 clinical used dental handpieces,according to the high-pressure steam sterilizer disposal procedure,were divided into four groups,with 30 handpieces in each group.Those only with high-pressure steam sterilizer process were chosen as the control group.Preheating 30 minutes,wipping the internal wall with 0.5% chlorine disinfectant before using high-pressure steam sterilizer and acting both of two steps were all chosen as the experimental groups.Each group was put in the interior,middle and exterior of the high-pressure steam sterilizer separately.The Rapid Readout Biological Indicators(3M Attest,the USA) were used to test the sterilizing effect.RESULTS The different methods of the high-pressure steam sterilizer had the significant differences to the handpieces sterilizing effect(P0.05).CONCLUSIONS To achieve ideal sterilization,it is suggested to wipe the internal wall and the temperature control probe head with 0.5% chlorine disinfectant and preheat the sterilizer for 30 minutes before put handpieces in the high-pressure steam sterilizer.

Development and application of hot steam electrical generator / 医疗卫生装备

The weakness of the boiler high pressure steam sterilizer is put forward.The development process and actual application value of the hot steam electrical generator is mainly introduced.

Comparación de resultados del proceso de esterilización con dos indicadores biológicos / Comparison of the results of the sterilization process with two biological indicators

Introducción: se consideran los indicadores biológicos una forma de aprobación para la validación y monitorización rutinaria de ciclos de esterilización, los indicadores biológicos convencionales utilizan tiras o discos con esporas del B. Stearthermophilus; por otra parte los indicadores biológicos de lectura rápida, están basados en una reacción enzimática flourecente capaz de proporcionar resultados en tres horas. Objetivo: comparar la efectividad de los indicadores biológicos convencionales y de lectura rápida durante procesos de esterilización a 121°C. Material y método: se realizó un estudio descriptivo en un periodo de 12 días, se identificó cada indicador con fecha, turno, número de esterilizador, y tiempo de esterilización. Se empaquetó por separado cada indicador en una bolsa de papel grado médico y se colocó dentro de un paquete de cuatro compresas de vientre envueltas en una compresa de tela doble, se realizó un preacondicionamiento de 10'y se expuso todo el material a 35' de esterilización con temperatura constante de 121°C, la lectura de cambio de color se observó en ambas pruebas; durante 24 hrs los resultados fueron anotados en una bitácora de control para indicadores biológicos. Resultados: los resultados tanto de indicadores biológicos convencionales como de lectura rápida fueron negativos para todas las pruebas después de 24 hrs de incubación. Conclusiones: la confiabilidad y eficiencia de ambos indicadores biológicos es de 100 %.

Introduction: the biologie indicators are consider an approbation way for the validation and routine inspect of sterilization cycles, the biologic conventional indicators, using dise with B spores stearthermophilus. Also the biologic indicators of fast reading to belong are according to enzyme fluorescent reaction to able for give results in three hours. Objective: compare the efficient of the biologic conventional indicators and fast reading during sterilization process at 121 °C. Method and material: it was make a descriptive study. In a period of 12 days it was identificate each indicator with date, turn, number of sterilization, and time of sterilization, they were packing by separate each indicator in a paper bag medical grade and they placed inside of a pack of four compress of a container, envolver in a compress of double cloth, it made a pre-conditioning of 10' and the material was expose to 35' of sterilizing to constant temperature of 121°C, the change reading color, it looked on both proofs during 24 hours the results were write on a logbook control to biologic indicators. Results: the answers of conventional indicators as the fast reading were negatives to all the proofs after 24 hours of incubation. Conclusion: the confidence and efficient of both biologic indicators is 100 %.

Several Sterilizing Methods for Paraffin Oil:A Comparative Experiment / 中华医院感染学杂志

OBJECTIVE To study effective and convenient method for paraffin oil sterilization.METHODS By using carrier qualitative germicidal test,to compare pressure steam sterilization,dry heat sterilization and cobalt-60(gamma)-ray radiation sterilization to test the sterilizing effect and operating procedure.RESULTS Pressure steam sterilization was unable to achieve 100% sterilizing effect,whether we extended the time or use the intermittent(sterilization).After dry heat or radiation sterilization processes,no microorganism was found.CONCLUSIONS Effect of sterilization with dry heat or radiation sterilization is trustable,but its packing,operation and equipment are requested strictly,and pressure steam sterilization may be not good for paraffin oil.

Causes Analysis and Solutions to B-D test failure / 医疗卫生装备

The B-D test is one of the important parameters for examining sterilizing effect of the pulsation vacuum pressure sterilizer,which has to be performed before application to sterilization.So before high pressure antisepsis,we must carry on the B-D test of the pulsation vacuum pressure steam sterilizer.With the causes analyzed,some solutions are put forward to enhance the reliability of B-D test failure.