Effect of tetracycline-HCl root conditioning on gingival epithelial cell attachment to root surface / 대한치주과학회지

The ultimate goal of periodontal therapy is directed to arresting the progression of the disease, and regenerating the fibrous attachment. In order to achieve such treatment aim, the plaque and calculus must be eliminated and the physiological conditions of the root surface must be changed to facilitate the attachment and migration of the new fibroblasts, The method of changing the proper root surface conditions to promote the healing of periodontal tissue involves mechanical procedures, such as scaling and root planing, and chemical procedures such as tetracycline-HCl. However, the formation of a long junctional epithelium was most frequently observed type of healing. Thus, the aim of this study was to examine in vitro the influence of surface conditioning of dentin by TC-HCl on human gingival epithelial cell attachment. Human gingival epithelial cells were obtained from healthy retromolar pad area(under the age 23 years). Seventy two teeth extracted from severe periodontitis were used as study material. To evaluate the epithelial cell attachment to dentin, the prepared specimen was divided to four groups. For the control group, only scaling and root planing were carried out, and for the test group, 1 to 3, the concentration of the TC-HCl was 50, 125 and 250mg/ml, respectively. After cell cultivation time of 1-, 3-. 24 hour, for the indirect quantitative assessment of gingival epithelial cell attached to dentin sample, the absorbance of epithelial cell unattached to dentin was measured. The results were as follows; 1. There was no statistically significant difference between scaling and root planing group and TC-HCl 50mg/ ml, 125mg/ml and 250mg/ml group about absorbance of unattached epithelial cell to dentin sample(p>0.5). 2. As time passes, the absorbance of unattached gingival epithelial cell to dentin sample was decreased statistically significant(p0.05) We concluded that there was similar effect on gingival epithelial cell attachment between TC-HCl conditioning on root surface and only scaling and root planing treatment

Effect of Tetracycline-HCl on superoxide anion generation by polymorphonuclear leukocytes / 대한치주과학회지

It has been believed that the increased release of free oxygen radicals(O2-, H2O2 and OH-) might be one of important factors in the pathogenesis of periodontal diseases. Polymorphonuclear leukocytes(PMNs) constitute the primary host resistance factor against infection. They are prominent cells in both the gingival tissue and gingival sulcus in most forms of periodontal disease. In response to invading microorganisms, the activated PMNs and macrophages generate free oxygen radicals, which play an important role in bacterial killing. The normal production of these free oxygen radicals is vital for the successful resistance of individuals to bacterial infection. However, the enhanced production of reactive oxygen species by accumulating PMNs may be detrimental to the host in certain disease states. This study was performed to evaluate the effect of Tetracycline-HCl(Tc-HCl) on generation of superoxide anion by PMNs. For the present study, 60ml of peripheral venous blood were obtained from systemically healthy subjects by venipuncture in median cubital vein and PMNs were separated by a one-step Ficoll-Hypaque gradient centrifugation method. PMNs were incubated with 1microgram/ml P.gingivalis strain A7436 LPS, 5% serum and Tc-HCl(5, 10, 50 and 100microgram/ml). The superoxide anion analysis was carried out by superoxide dismutase-inhibitable cytochrome C reduction method using Microplate autoreader(BIO-TEK(TM) Instrument Inc.). The difference of superoxide anion generation between control group and Tc-HCl group was statistically analyzed by paired t-test. The superoxide anion generation in the course of time after treatment with Tc-HCl was analyzed by ANOVA, and the superoxide anion generation in the course of time after treatment with various concentrations of Tc-HCl was analyzed by Repeated Measurement test. The results were as follows: 1. Superoxide anion generation by PMNs was significantly decreased by Tc-HCl(p0.05). 3. Superoxide anion generation by PMNs was significantly decreased in the course of incubation time after treatment with Tc-HCl(p<0.05). The results demonstrate that the Tc-HCl inhibit superoxide anion generation by PMNs and the inhibitory effects depend on the exposure time rather than the concentration of Tc-HCl.